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ismokeweed
Stranger

Registered: 11/19/05
Posts: 286
Last seen: 14 years, 9 months
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going straight from spore printing to syringe..(in the mmgg)
#5573717 - 04/30/06 11:10 AM (17 years, 8 months ago) |
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"If it is your intent to use a mushroom to immediately generate inoculant for a successive crop, you can eliminate some of the above steps and reduce the risk of contamination.
Poke a small nail hole in the cap of a wide mouth jar. Cover the nail hole with a piece of electrical tape. A 1/2 pint canning jar similar to that used for the cultures is fine. Sterilize both the jar and the lid.
Place the harvested cap in the sterilized, wide mouth jar. Seal the top with the lid and wait until a spore print has been generated on the bottom of the glass. Open the jar and remove the mushroom cap. Add 3/4 cup of sterilized water. Seal the top of the jar with the lid and shake the jar. The spores need to be mixed well into the water. This procedure elements the need to transfer spores from a print to some container to make the solution. The spore print is generated inside the container and the only extra step is adding water. It also has the benefit of making a large amount of solution that is easy to use.
Now, any time you need inoculant, you can shake the jar and peel the tape back to expose the nail hole in the lid. Simply insert the needle of the syringe into the inoculant and pull some into the syringe. The syringe needs to be sterile or you risk contaminating the entire jar of solution. The solution will keep longer if you refrigerate it. You still should keep some spore prints on paper because it is possible to contaminate the entire jar of inoculant if you make a mistake."
has anybody tried this? i definitely want to give it a shot for my first time printing. it seems to me to be a lot easier than making a print and then having to get it in a syringe. what do you guys think?
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FooMan



Registered: 02/02/05
Posts: 8,957
Loc: Earth
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Re: going straight from spore printing to syringe..(in the mmgg) [Re: ismokeweed]
#5573741 - 04/30/06 11:21 AM (17 years, 8 months ago) |
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It works. Agar and Magash have both posted similar methods. Using an ultrasonic cleaner and a couple drops of jet dry, you can disperse the spores very evenly.
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Quick WBS Prep
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Oatman2000
-=Outa Space=-



Registered: 05/10/05
Posts: 2,877
Loc: Planetary Nebula
Last seen: 1 year, 4 months
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Re: going straight from spore printing to syringe..(in the mmgg) [Re: FooMan]
#5576920 - 05/01/06 11:28 AM (17 years, 8 months ago) |
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i have had issues with the jet dry foaming up. but this tenique is great.
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Spawning to COIR
My Chocolate Recipe
WBS QUART SPAWN JAR PREPERATION ----------------------------
4-PO-DMT; 4-phosphoryloxy-N,N-dimethltryptamine
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NCDOC0623414
nerdysouth


Registered: 08/07/10
Posts: 320
Loc: nerdysouth
Last seen: 10 years, 9 months
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Re: going straight from spore printing to syringe..(in the mmgg) [Re: ismokeweed]
#13240761 - 09/24/10 01:50 PM (13 years, 4 months ago) |
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im tryin this asap and will post my results
-------------------- Froggy Style!
My pad or yours?
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NineInchNails
Stranger

Registered: 03/01/09
Posts: 1,190
Last seen: 1 year, 24 days
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Re: going straight from spore printing to syringe..(in the mmgg) [Re: NCDOC0623414]
#13240802 - 09/24/10 01:59 PM (13 years, 4 months ago) |
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I never had good luck using Jet Dry. Jet Dry definitely does disperse the spores evenly with no clumps, but that stuff is very corrosive! Months later I tried using one of those refrigerated syringes where a drop of jet dry was used and I think that stuff killed the spores. Not a single spore germinated. I reinoculated over a week later and still no germination. I even inoculated a 3rd time and still no luck.
I made another spore syringe using 1 or 2 drops of KY with the syringe and very poor results too. Perhaps KY uses some kind of preservatives that killed the spores.
Finally I just said screw it and used just spores & water. That syringe showed germination in less than 24 hrs!
Just spores & water for me from now on.
Edited by NineInchNails (09/24/10 02:00 PM)
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