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shymanta
Mad Scientist


Registered: 01/27/05
Posts: 907
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Agar without darkness
#5571206 - 04/29/06 05:48 PM (17 years, 8 months ago) |
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Check out this clone on agar (PDA). It got a little too much light.
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RogerRabbit
Bans for Pleasure


Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 11 months, 3 days
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Re: Agar without darkness [Re: shymanta]
#5571257 - 04/29/06 06:11 PM (17 years, 8 months ago) |
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It's not the light, it's from letting it sit too long. A petri dish will fully colonize in two weeks or less. I usually transfer away when it's 2/3 colonized to make sure to get that rapid growth transferred into my grains. If you let a petri dish sit for a week or two after they fully colonize, that happens. It proves you have a fruiting strain.
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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shymanta
Mad Scientist


Registered: 01/27/05
Posts: 907
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Re: Agar without darkness [Re: RogerRabbit]
#5573585 - 04/30/06 09:54 AM (17 years, 8 months ago) |
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So a dish would do that even if kept in complete darkness? This one was on top of a stack of three that were put on a shelf in open light and forgotten about. The other two have knots, pins and bruising all over their surface. They were left over stock inocula and I have no idea how long they've been sitting there.
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RogerRabbit
Bans for Pleasure


Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 11 months, 3 days
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Re: Agar without darkness [Re: shymanta]
#5573601 - 04/30/06 10:05 AM (17 years, 8 months ago) |
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It's irrelevant what would happen in total darkness, because petri dishes must be inspected daily and mycelium transferred at the right times. Light has no effect on colonizing mycelium, good or bad. There is absolutely no reason to keep petri dishes in the dark. What you see is totally normal. Pins form on agar all the time if they are left too long. Agar/petri dishes are not for expanding mycelium. They are for germinating spores and isolating away from contaminants and for isolating strains. I consider the life of mycelium in a petri dish to be around three weeks at room temperature. After that, anything can happen, such as contaminants or as you got, fruiting. It's nothing wrong or unusual. Below on the left are fruits forming from mexicana jalisco strain sclerotia, and on the right, cubensis fruiting from agar on a petri dish. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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shymanta
Mad Scientist


Registered: 01/27/05
Posts: 907
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Re: Agar without darkness [Re: shymanta]
#5573789 - 04/30/06 11:37 AM (17 years, 8 months ago) |
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Check out this isolate. Sclerotia at the top.
Edited by shymanta (05/15/06 07:22 PM)
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