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OfflineMobius_Strip
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Agar Isolation School
    #5457821 - 03/30/06 02:06 AM (15 years, 6 months ago)

I've been looking for concrete examples of ropey mycelium and rizomorphic strains. I understand that it's some hardy shit and that's what I want, but what does it look like from strain to strain. Is there a really consistent appearance besides the written descriptors of ropey, strandy and rizomorphic?

I've gotten really good at making agar plates and growing out a few strains...I can even isolate pretty well...but...what the hell should I be isolating? I understand that it should be "ropey" and "hardy". Hell, I have a strain of pan cyan goliath thats happy as a pig in shit and has been for weeks, in the center of a bacterial bloom that has occupied the rest of the plate and destroyed every other strain in its way. I get what it means to isolate contam resistant strains. That one strain is standing it's ground like a fuzzy tower of "fuck you" over bacteriaville and seems to go the extra mile. In the meantime I have a multispore F strain plate thats ready to bust and I have no idea which parts are desireable. Should I just isolate each different looking strain and try fruiting them for the best results or is there a clear pattern that I should be looking for to acheive a hardy, prolific, contam resistent AND potent strain?

I've seen stamets depiction of rizomorphic mycelium on agar, it looks pretty distinct. The mycelium spreads like lightning in thick, well delineated, bolt-like patterns. What about other mushroom varieties or strains? I've got strandy looking shit in my F plate that looks spidery, thick, fuzzy, strandy and spreads fast but looks nothing like stamets depiction of "rizomorphic". Is this desireable over the super-duper-fuzzy, thick, blotchy, bulbous, stuff that spreads fast and blooms into patchy mycelium balloons? Does anyone have a good step by step pictorial of sectoring, rizomorphic growth and isolating different strains on agar?

Is there a universal truth or is it all about isolating strains and fruiting then out objectively?


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The smart way to keep people passive and obedient is to strictly limit the spectrum of acceptable opinion, but allow very lively debate within that spectrum - even encourage the more critical and dissident views. That gives people the sense that there's free thinking going on, while all the time the presuppositions of the system are being reinforced by the limits put on the range of the debate
-Noam Chomsky


Edited by Mobius_Strip (03/30/06 02:43 AM)


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OfflineBPJohnny
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Re: Agar Isolation School [Re: Mobius_Strip]
    #5463277 - 03/31/06 11:31 AM (15 years, 6 months ago)

a good resource is "the mushroom cultivator" there are 2 major classes of mycelial sectors, rhizomorphic wich is strandy and tomentose wich is cottony. a third type wich is strandy is a mycelial growth that grows linearly, this is not desired.


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InvisibleHippie3
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Re: Agar Isolation School [Re: Mobius_Strip] * 1
    #5464735 - 03/31/06 06:51 PM (15 years, 6 months ago)

some nice isolates of the PE strain of cubensis







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OfflineMobius_Strip
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Re: Agar Isolation School [Re: Hippie3]
    #5514674 - 04/14/06 04:06 AM (15 years, 6 months ago)

Thanks Hippie3, that's awesome? Why does the second picture look different than the other two? Is the second photo a less desireable form of "rizomorphic"?


--------------------
The smart way to keep people passive and obedient is to strictly limit the spectrum of acceptable opinion, but allow very lively debate within that spectrum - even encourage the more critical and dissident views. That gives people the sense that there's free thinking going on, while all the time the presuppositions of the system are being reinforced by the limits put on the range of the debate
-Noam Chomsky


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Offlinethenewguy05
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Re: Agar Isolation School [Re: Mobius_Strip]
    #5525258 - 04/17/06 04:24 PM (15 years, 6 months ago)

mycelium varies a lot even from the same species.


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OfflineDocPsilocybin
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Re: Agar Isolation School [Re: Mobius_Strip]
    #5525703 - 04/17/06 06:31 PM (15 years, 6 months ago)

Mmm, it's sort of speculation to which type of mycelium will give you better fruits. To select for size/potency/etc you're better off to clone off of your fruits.

But isolating mycellium can give you resistant/aggressive and fast colonizing strains. There's just no guarantee they will produce good flushes although maybe there is a relationship. Experimentation I guess.

I say isolate as many differences as you can. Large fruits, small fruits, healthy fruits, weird fruits, fruits from large flushes, fuzzy mycellium, ropey mycellium, bacteria resistant mycellium, etc. Eventually you'll find something to suit your needs.


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OfflineMobius_Strip
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Re: Agar Isolation School [Re: DocPsilocybin]
    #5537984 - 04/20/06 04:44 PM (15 years, 6 months ago)

That's probably the most helpful advice so far. Thanks Doc. I'll just keep isolating, fruiting and cloning until I get some good strains. Mileage may vary...I can live with that.


--------------------
The smart way to keep people passive and obedient is to strictly limit the spectrum of acceptable opinion, but allow very lively debate within that spectrum - even encourage the more critical and dissident views. That gives people the sense that there's free thinking going on, while all the time the presuppositions of the system are being reinforced by the limits put on the range of the debate
-Noam Chomsky


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InvisibleHippie3
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Re: Agar Isolation School [Re: Mobius_Strip]
    #5538003 - 04/20/06 04:49 PM (15 years, 6 months ago)

Quote:

Mobius_Strip said:
Thanks Hippie3, that's awesome? Why does the second picture look different than the other two? Is the second photo a less desireable form of "rizomorphic"?




no, it's a transfer of agar from one plate to another,
2nd generation. that's why the chunk in the middle, etc.


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InvisibleCorporal Kielbasa
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Re: Agar Isolation School *DELETED* [Re: DocPsilocybin]
    #5539246 - 04/20/06 11:32 PM (15 years, 6 months ago)

Post deleted by Corporal Kielbasa

Reason for deletion: .



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OfflineRogerRabbitM
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Re: Agar Isolation School [Re: Corporal Kielbasa] * 1
    #5539582 - 04/21/06 01:21 AM (15 years, 6 months ago)

The idea with strain isolation is to make a dozen or so isolates, then fruit each one to find that stellar performing strain. Once you have a really good performing strain, with care you can continue to use it forever, making the time spent isolating worth the effort.
RR


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InvisibleOldSpice
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Re: Agar Isolation School [Re: RogerRabbit]
    #5542780 - 04/22/06 01:58 AM (15 years, 6 months ago)

Thats what ive done with isolate Hippie sent me


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OfflineKaptKid
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Re: Agar Isolation School [Re: OldSpice]
    #5552001 - 04/24/06 08:47 PM (15 years, 6 months ago)

:sun:


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OfflineMycomyth
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Re: Agar Isolation School [Re: RogerRabbit]
    #5566395 - 04/28/06 11:33 AM (15 years, 5 months ago)

Quote:

RogerRabbit said:
The idea with strain isolation is to make a dozen or so isolates, then fruit each one to find that stellar performing strain.  Once you have a really good performing strain, with care you can continue to use it forever, making the time spent isolating worth the effort.
RR





How long can a Master plate be maintained? What is the best storage method to retard growth, yet keep a culture alive?


:bow:
M


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