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OfflineFeelers
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Re: Too far-fetched? [Re: fastfred]
    #5594600 - 05/05/06 03:05 PM (18 years, 18 days ago)

I'm making a polyploid of salvia - it will hopefully reinstall its fertility (my lecturer thinks this will happen too) and it will be grow bigger, faster and more potent ect.

The thing is you cant use cholchine - as there arent many seeds around and if you can get them it'd cost thousands.


But as for this mushroom fusants, tell me what "crosses" would people like to see? After some suggestions I'll email my lecturer and see if I can do a fusion for my mycology project. :laugh:
I'm thinking since all mycelium looks pretty much the same - it probably wouldnt matter if an "active" made it into the mix. haha.

Say, what happens if you fuse dikaryons? Do mushrooms function ok/better as polyploids?

Ok so what are some closely related species of edible easy to grow mushrooms to the actives?

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InvisibleBlimeyGrimey
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Re: Too far-fetched? [Re: Feelers]
    #5600019 - 05/07/06 02:30 AM (18 years, 16 days ago)

there are some Psilocybe species that ARENT active, and remember you could also try an active-Conocybe/non-active-conocybe mix etc.
Breeding within the same genus seems like it will make the task easier to me.

here's a cross i'd like to see.

Gymnopilus ventricosus x Gymnopilus spectabilis

spectabilis is active and ventricosus are huge.
seems like a good combo to me.

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OfflineFeelers
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Re: Too far-fetched? [Re: BlimeyGrimey]
    #5602858 - 05/07/06 09:45 PM (18 years, 16 days ago)

Can you get a monokayotic culture from dikaryotic mycellia? I'm asking because say I dont have access to spores how can I get a monokaryon from a clonal culture.
Is this possible? I'm geussing it isnt?

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OfflineWorkmanV
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Re: Too far-fetched? [Re: Feelers]
    #5604306 - 05/08/06 09:18 AM (18 years, 15 days ago)

It can happen spontanously, but I am not certain if there is a method to induce it. I haven't really looked into that in detail since the isolation of single spores is pretty straight forward.


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OfflineFeelers
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Re: Too far-fetched? [Re: Workman]
    #5605975 - 05/08/06 06:02 PM (18 years, 15 days ago)

Well I have emailed my lecturer if I can do a protoplast fusion, so I'm just waiting for a response.
The cool thing is he'll be real stoked someone wants to do such an interesting project, so he'll probably help me a lot.

Crazy guy my lecturer - he's in a ZZ TOP live video climbing the wall smashed on acid. :laugh:
Now he's a respectable looking man with glasses and white hair. Definately my favourite lecturer. I might point him in the shroomerys direction.

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Invisiblefastfred
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Re: Too far-fetched? [Re: Feelers]
    #5631173 - 05/15/06 02:15 AM (18 years, 8 days ago)

You asked about haploidization... Haploidization can be caused by many factors. Errors in cell division, stressful environment, lack of nutrients, chemicals, etc.. The standard way to induce it is by using benomyl.

Here is a quote... "...colonies can be induced to undergo haploidization by treatment with parafluorophenylalinine, of benomyl, UV or X-rays. (Stipiczki and Ferenczy, 1977; Peberdy et al., 1977; Sarachek et al., 1981)."

Sorry for not providing a complete citation, if you're really interested I can look it up for you.


-FF


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It drinks the alcohol and abstains from the weed or else it gets the hose again. -Chemy

The difference between the substances doesn't matter. This is a war on consciousness, on our right to the very essence of what we are. With no control over that, we have no need to speak of freedom or a free society. -fireseed

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I find the whole "my drug should be legal but yours should be illegal" mindset disgusting and hypocritical. It's what George Bush and company do when they drink a cocktail and debate the best way to imprison marijuana users. -Diploid

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