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Offlinewiggles
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Sporeprint to Agar to LC to Innoc
    #5562380 - 04/27/06 10:49 AM (17 years, 9 months ago)

Alrighty, so here is my plan. I've got 2 prints, and I'm planning on putting them on PDA to make sure that they're contam free (they should be since they haven't left the sealed desiccant chamber since the night I made them). From there I was planning on storing the petri dishes until they were needed, and from there use an inoculation loop to place some myc into an LC.

Does a transfer from agar to LC work alright? I figure if I do it in this way I should be able to get an almost infinite number of innocs from those two spore prints (or at least more than I could ever conceivably use).

Also, I'm planning on getting a print of mexicana A to develop sclerotia. How well does this stuff work on agar? I've seen pictures of sclerotia forming ON agar, and I'd think this would be counterproductive to what I'm trying to do (keep a supply of spores and myc always on hand). Also, would the sclerotia try to form in an LC as well?


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Offlineiamyour_messiah
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Re: Sporeprint to Agar to LC to Innoc [Re: wiggles]
    #5562987 - 04/27/06 02:11 PM (17 years, 9 months ago)

I dunno I'm quite a noob myself, but personally it would seem less time consuming if you just went straight from sporeprint to LC. Like you said, if they have been in the drying chamber all that time it's unlikely they are contamed. Either way, even if you find out that they are, then you'll know that, but it won't change the fact.

If you had to go using up a whole sporeprint for making an LC then I'd understand why you'd wanna go to agar first. Since you only need a small part of the print for one LC, though, I'd make like 3 of them and that in itself will already supply you with more innoculant than you can probably make use of.


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Offlinethenewguy05
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Re: Sporeprint to Agar to LC to Innoc [Re: iamyour_messiah]
    #5562998 - 04/27/06 02:14 PM (17 years, 9 months ago)

i agree. go from spore print to LC


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InvisiblePremedman1
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Re: Sporeprint to Agar to LC to Innoc [Re: thenewguy05]
    #5563031 - 04/27/06 02:24 PM (17 years, 9 months ago)

^^^^^ Unless you plan on isolating a single substrain.


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OfflineSmirnoff
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Re: Sporeprint to Agar to LC to Innoc [Re: Premedman1]
    #14701966 - 07/01/11 01:42 PM (12 years, 6 months ago)

^^^^^^

in this case would it still work?


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OfflineFuckMeRunnin
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Re: Sporeprint to Agar to LC to Innoc [Re: Smirnoff]
    #14702041 - 07/01/11 02:01 PM (12 years, 6 months ago)

I recently saw a Tek by a TC that they made LC by knocking up a grain jar, waiting for colonization, squirting water into grain jar, swishing around, sucking water back out and into an lc....It was interesting and I assume you could do it that way, then use the grain to G2G? Just a suggestion...


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Offlineely2121
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Re: Sporeprint to Agar to LC to Innoc [Re: FuckMeRunnin]
    #14703695 - 07/01/11 07:53 PM (12 years, 6 months ago)

Such an old thread!  :oldthread:

Old info I think but if you go from print to LC you are at high risk for contams and not only that, you wouldn't know until you inoculated a jar and it is too late.

Print to agar is the way to go.  Transfer a segment of aggressive growth to a grain jar like FMR said above.  Grain LC is the way to go IMO.


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