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InvisibleLSD_rules
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Registered: 02/26/06
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Mycelium Transfer
    #5558442 - 04/26/06 12:12 PM (17 years, 9 months ago)

once after my cakes are colonized, i usually take a piece off of the cakes and use that to colonized another cake, which so far gave me no trouble at all. my question is, everytime i keep transferring the mycelium, does if effect the potency of the mushrooms when they are fruiting? this is probably a stupid question but im quite curious :smile:


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InvisibleLSD_rules
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Re: Mycelium Transfer [Re: LSD_rules]
    #5558456 - 04/26/06 12:16 PM (17 years, 9 months ago)

any thoughts?


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OfflineSinthetic
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Re: Mycelium Transfer [Re: LSD_rules]
    #5558464 - 04/26/06 12:19 PM (17 years, 9 months ago)

Eventually it will get weak and wont even be able to colonize. What you want to do is grow a cake from spores or young mycelium. When It's finished put it in the fridge and it will go into stasis. Then just take a little piece off whenever you need some. Or just start working with agar.


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Invisiblecreamcorn
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Re: Mycelium Transfer [Re: LSD_rules]
    #5558467 - 04/26/06 12:20 PM (17 years, 9 months ago)

you need to chill on the bumping of posts minutes after they're made :-)

i got all your answers today anyway haha...

yeah you can do that to an extent.  after a couple times its best to start up fresh again.  a phenomena called senscense kicks in.. between the mycellium living to an "old age" and genetic mutations after its grown out for so long you can start to see a decrease in the quality of the strain.... however this old mycellium, if it fruits, will still produce spores that are "brand new" genetically speaking, so you can still print and re-start at that point without ill effects.

whether or not it directly effects potency depends, it depends on how its degraded... many times it does though.  there's a certain number of times you can safely transfer, i forget the number off hand, but look around for info on senscense and you'll get a way better explanation of whats going on and what to do/not to do than i just gave :smile:


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OfflineSour_D
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Re: Mycelium Transfer [Re: LSD_rules]
    #5558469 - 04/26/06 12:20 PM (17 years, 9 months ago)

I really don't think that it does, atleast not like herb does after cloning repeatedly, but I am a noob as well, so I am not the best source...
I also wanted to add to this question...

I keep hearing potency of cubies is the same amoung all cubies and it depends on casing material... What is the best casing material for highest potency in cubies?? Or does that have to do w/race (orissa's = poo lovers)???


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OfflineSour_D
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Re: Mycelium Transfer [Re: Sour_D]
    #5558472 - 04/26/06 12:22 PM (17 years, 9 months ago)

poo is not a casing, man am I a noob...


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Invisiblecreamcorn
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Re: Mycelium Transfer [Re: creamcorn]
    #5558474 - 04/26/06 12:22 PM (17 years, 9 months ago)

P.S. yeah, like sinthetic mentions if you keep a cake around in the fridge and use it as your "master" and break a chunk off that each time, your myc is starting again from that point. when i say "old age" its not so much speaking in a time sense, but more from the sense of how many times the mycellium cells have divided and extended out...


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OfflineSour_D
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Re: Mycelium Transfer [Re: creamcorn]
    #5558482 - 04/26/06 12:24 PM (17 years, 9 months ago)

so after a couple grain transfers one would want to take a print and start fresh?


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Invisiblecreamcorn
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Re: Mycelium Transfer [Re: Sour_D]
    #5558485 - 04/26/06 12:25 PM (17 years, 9 months ago)

Quote:

Sour_D said:
I also wanted to add to this question...

I keep hearing potency of cubies is the same amoung all cubies and it depends on casing material...  What is the best casing material for highest potency in cubies??  Or does that have to do w/race (orissa's = poo lovers)???




not casing material, but substrate material.  you need an optimal mix of nutrients for optimal alkaloid production.  horse poo seems to come closest to that and is a great choice.  however potency varies based on all of your other growing parameters, a well grown brf shroom can be more potent than a poorly grown h/poo one.  i say just grow lots of them, then you won't really give a crap about the tiny percentage swings in potency, you can just eat lots more of them  :smile:


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InvisibleLSD_rules
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Re: Mycelium Transfer [Re: Sour_D]
    #5558489 - 04/26/06 12:26 PM (17 years, 9 months ago)

ya, i get quite impatient in waiting for answers to my questions, nonetheless, they still get answered :smile:  i do still make prints, but have been transferring mycelium for awhile so i wouldn't need to use my prints. but seeing how ive transferred for awhile, perhaps i should use some of those prints. and to answer the last post, some mushrooms are more potent than others. but im not sure if the casing has any effect on the casing.  so far azurescens has been the strongest, most potent cube i have experienced, IMO


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Invisiblecreamcorn
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Re: Mycelium Transfer [Re: Sour_D]
    #5558490 - 04/26/06 12:27 PM (17 years, 9 months ago)

Quote:

Sour_D said:
so after a couple grain transfers one would want to take a print and start fresh?




exactly.

or by keeping one grain jar around, under refrigeration, and make transfers from that each time for as long as its viable (up to a few months in some cases). then each of those transfers are a "first generation" - same goes for LC, and it seems to store longer under refrigeration.


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OfflineSour_D
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Re: Mycelium Transfer [Re: creamcorn]
    #5558505 - 04/26/06 12:29 PM (17 years, 9 months ago)

sounds kinda risky, wouldn't want that jar to get contaminated...


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Invisiblecreamcorn
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Re: Mycelium Transfer [Re: LSD_rules]
    #5558511 - 04/26/06 12:30 PM (17 years, 9 months ago)

Quote:

LSD_rules said:
ya, i get quite impatient in waiting for answers to my questions, nonetheless, they still get answered :smile: 

so far azurescens has been the strongest, most potent cube i have experienced, IMO




yeah you've been bumping faster than i can type :-)

and P.S. azures aren't cubes.  that's why they're stronger, they're a different species all together (one that's more difficult to grow, which is why the focus is usually on cubes)


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Invisiblecreamcorn
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Re: Mycelium Transfer [Re: Sour_D]
    #5558518 - 04/26/06 12:33 PM (17 years, 9 months ago)

Quote:

Sour_D said:
sounds kinda risky, wouldn't want that jar to get contaminated...




it shouldn't get contaminated as long as its fully colonized and well sealed. you're right though - food refrigerators are loaded with all kinds of nasties... and every time you open your master jar up you're risking airborn contams landing in there - while they might not be able to take a foothold in the already colonized jar, if you transfer it into a new substrate it will have its chance to do damage then. transfers are great to speed things up and are definitely a lazy way out, but yeah there's added contam risk.


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OfflineSour_D
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Re: Mycelium Transfer [Re: creamcorn]
    #5558528 - 04/26/06 12:35 PM (17 years, 9 months ago)

I guess the answer would be to get 2 or 3 masters goin per race.... have a couple spare prints just in case.. cover all your bases... Hope you have nice fridge space!!


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OfflineSinthetic
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Re: Mycelium Transfer [Re: Sour_D]
    #5558576 - 04/26/06 12:50 PM (17 years, 9 months ago)

This is why alot of hardcore growers have many fridges.


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InvisibleDIRTYMAN
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Re: Mycelium Transfer [Re: Sinthetic]
    #5558870 - 04/26/06 02:26 PM (17 years, 9 months ago)

Or use glove boxes with a clean work area  :smile:


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OfflineSour_D
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Re: Mycelium Transfer [Re: DIRTYMAN]
    #5558891 - 04/26/06 02:34 PM (17 years, 9 months ago)

I sanitize the hell out of a small bathroom usually don't have problems, shit happens though....


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