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time2
Stranger
Registered: 03/23/06
Posts: 357
Last seen: 16 years, 2 months
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How much LC in the JAR?
#5553581 - 04/25/06 12:43 AM (17 years, 9 months ago) |
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How much Fully Colonized Liquid Culture would I put in a 1/2 gallon WBS Jar? How long would it take to colonize and anyone ever did this?
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DaCultivater
Stranger
Registered: 10/08/05
Posts: 289
Last seen: 15 years, 10 months
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Re: How much LC in the JAR? [Re: time2]
#5553603 - 04/25/06 12:52 AM (17 years, 9 months ago) |
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i did it... jars stalled at 1/2-3/4 don't do it
do gtg with 1/2 gals
-------------------- as they said in some movie(i don't remember which one); a belief is a dangerous thing, people will kill over it; instead of believing you need to find your facts and then have an opinionated idea that you are willing to change in light of new evidence
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ShroomInduced
I read too manymycology books


Registered: 04/19/05
Posts: 309
Loc: A giant mushroom in my he...
Last seen: 12 years, 5 months
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Re: How much LC in the JAR? [Re: DaCultivater]
#5553640 - 04/25/06 01:11 AM (17 years, 9 months ago) |
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I would put in about 6-8 CC of liquid culture, try to suck up as much MYC as you can in the syringe so you get allot in it. After you put inject it, shape it up really well.
-------------------- That's a deep kiss too, like the Europeans. You know, the French, they have to unhinge their jaw to show love. Computer Fan Tek Vote for you favorite strain of cubes 97 choices!
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time2
Stranger
Registered: 03/23/06
Posts: 357
Last seen: 16 years, 2 months
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ok so 8 cc's.... a few week colonization time or what?
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fresh313
journeyman


Registered: 09/01/03
Posts: 2,537
Last seen: 12 years, 9 months
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Re: How much LC in the JAR? [Re: time2]
#5553845 - 04/25/06 02:27 AM (17 years, 9 months ago) |
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yah id do what the other guy said shoot up a quart jars with like 2cc's lc, then do g2g for the 1/2 gallons.
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GnuBobo
Frilly Cuffs Extraordinaire


Registered: 06/17/04
Posts: 43,754
Loc: Charisma
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Quote:
ShroomInduced said: I would put in about 6-8 CC of liquid culture, try to suck up as much MYC as you can in the syringe so you get allot in it. After you put inject it, shape it up really well.
I had lots of problems with wetspot bacterial problems with LC inoculations with grain jars until I started putting in about a 1/4 cup of vermiculite into a quart grain jar. I just mix up the grain and vermiculite in a large mixing bowl--eyeballing the mix--and then load the jars up and PC them. The verm seems to balance out the moisture content--this has mostly been playing around with edibles, but it should work with any species, really. Shake the jars up and distribute the LC mycelium as best you can. I just apparently am stupid in getting LC mycelium up into the syringe without getting a few CCs of water, too. The verm helps me out that way.
-------------------- Jerry Garcia. JERRY GARCIA! JERRY GARCIA!!!!
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ShroomInduced
I read too manymycology books


Registered: 04/19/05
Posts: 309
Loc: A giant mushroom in my he...
Last seen: 12 years, 5 months
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Re: How much LC in the JAR? [Re: GnuBobo]
#5554067 - 04/25/06 05:47 AM (17 years, 9 months ago) |
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Who said anything about being stupid.... LoL its not that hard to put the needle by a chuck of floating MYC and sucking it up.... Ive even herd of people filtering out the liquid to get a higher concentration... But sorry for posting what works! If you soaked your grains, wetspot shouldn't be a problem. BUT I MIGHT JUST BE STUPID LIKE GnuBoBo...
-------------------- That's a deep kiss too, like the Europeans. You know, the French, they have to unhinge their jaw to show love. Computer Fan Tek Vote for you favorite strain of cubes 97 choices!
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time2
Stranger
Registered: 03/23/06
Posts: 357
Last seen: 16 years, 2 months
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Thanks shroominduced
"then do g2g for the 1/2 gallons"
What is g2g?
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Razman
Stranger

Registered: 07/07/04
Posts: 514
Last seen: 9 years, 5 days
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Re: How much LC in the JAR? [Re: time2]
#5554899 - 04/25/06 01:07 PM (17 years, 9 months ago) |
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"grain to grain" transfer
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