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OfflineArchMagus
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How many days after innoc. and nothing... do you give up?
    #5552189 - 04/24/06 07:27 PM (17 years, 9 months ago)

How many days after inoculation and you see nothing, would you then toss it out?

after 5 days? 8? 10? .... ?


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Offlinecokaznrebel
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Re: How many days after innoc. and nothing... do you give up? [Re: ArchMagus]
    #5552196 - 04/24/06 07:29 PM (17 years, 9 months ago)

I wait about 2 weeks, for both spores and LC for signs of growth.


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Invisibleboomerman
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Re: How many days after innoc. and nothing... do you give up? [Re: ArchMagus]
    #5552199 - 04/24/06 07:29 PM (17 years, 9 months ago)

i waited 5 weeks on a few jars and there still not done yet


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OfflineBPJohnny
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Re: How many days after innoc. and nothing... do you give up? [Re: boomerman]
    #5552208 - 04/24/06 07:32 PM (17 years, 9 months ago)

i could take a while for spores to germinate and i definitely would not

give up after only 10 days!


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OfflineArchMagus
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Re: How many days after innoc. and nothing... do you give up? [Re: boomerman]
    #5552214 - 04/24/06 07:34 PM (17 years, 9 months ago)

Is it normal that I notice a corn smell from my cracked corn?


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Offlinefuturewolf42o
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Re: How many days after innoc. and nothing... do you give up? [Re: ArchMagus]
    #5552286 - 04/24/06 07:51 PM (17 years, 9 months ago)

Quote:

ArchMagus said:
Is it normal that I notice a corn smell from my cracked corn?




are you kidding?


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cheese-it!


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Offlinejtk
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Re: How many days after innoc. and nothing... do you give up? [Re: futurewolf42o]
    #5552308 - 04/24/06 07:55 PM (17 years, 9 months ago)

if u dont see any sign of growth after 8-10 days then id start over. Its going to dry out and take forever to finish if it takes that long to even start.


--------------------
Don't let your brain tell your mind it can't feel


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Offlinealsnow469
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Re: How many days after innoc. and nothing... do you give up? [Re: ArchMagus]
    #5552318 - 04/24/06 07:58 PM (17 years, 9 months ago)

If you send me $20.. I'll tell You :cool:


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OfflineArchMagus
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Re: How many days after innoc. and nothing... do you give up? [Re: jtk]
    #5552339 - 04/24/06 08:03 PM (17 years, 9 months ago)

Do I blame the spore supplier then and get more spores free saying the syringe was contaminated?


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OfflineBPJohnny
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Re: How many days after innoc. and nothing... do you give up? [Re: ArchMagus]
    #5552356 - 04/24/06 08:07 PM (17 years, 9 months ago)

umm no not unless they WERE at fault if you have any more spore solution left make a LC to see if the spores are viable


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OfflineStainBlue
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Re: How many days after innoc. and nothing... do you give up? [Re: ArchMagus]
    #5552370 - 04/24/06 08:12 PM (17 years, 9 months ago)

No you would not do that because no growth does not necessarily mean contamination. If it was contamination you would probably see a little bit of growth anyway because of the foreign spores in the jars or a funny smell due to bacteria.

And to add to that there are other factors to consider: Maybe the substrate in the jar isn't moist enough, maybe they're being incubated at the wrong temp, if there is contamination maybe you didn't sterilize right, or maybe you didnt wait long enough to inoculate the jar. There are far more possibilites that are your fault compared to it being the vendors fault. Not that I'm saying it is your fault you aren't seeing growth but the odds are against you.

I would start again and this time pay a whole lot more attention to the details such as sterile technique, incubator temp, moist level of the substrate, waiting for the jars to cool, and other important things I am forgetting to mention.


--------------------
Peace,
StainBlue


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OfflineIGnosticAbhorI
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Re: How many days after innoc. and nothing... do you give up? [Re: ArchMagus]
    #5552383 - 04/24/06 08:14 PM (17 years, 9 months ago)

Quote:

ArchMagus said:
Do I blame the spore supplier then and get more spores free saying the syringe was contaminated?




Blame them for what? You're Studying these only right?..:smirk:

After all, i'm SURE you've read their disclamier..."FOR STUDY ONLY"

-Gnostic


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OfflineStainBlue
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Re: How many days after innoc. and nothing... do you give up? [Re: IGnosticAbhorI]
    #5552405 - 04/24/06 08:17 PM (17 years, 9 months ago)

Well put Gnostic -- I didnt even think of that point.


--------------------
Peace,
StainBlue


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OfflineIGnosticAbhorI
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Re: How many days after innoc. and nothing... do you give up? [Re: StainBlue]
    #5552427 - 04/24/06 08:21 PM (17 years, 9 months ago)

However...If you can pin-point where the fault was...And if Indeed if was the vendors...PM them about it and see what they say. Most of them are nice and really easy going about it. :thumbup:

Just don't EXPECT anything, after all, you're just another mycologist studying some spores....:grin:

-Gnostic


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OfflineArchMagus
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Re: How many days after innoc. and nothing... do you give up? [Re: IGnosticAbhorI]
    #5552504 - 04/24/06 08:36 PM (17 years, 9 months ago)

At what point does one give up on this hobby? If you try like 5 times and can never get anywhere, just give up?


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InvisibleDarkenshroom
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Re: How many days after innoc. and nothing... do you give up? [Re: ArchMagus]
    #5552540 - 04/24/06 08:42 PM (17 years, 9 months ago)

Whether one would give up after repetative tries or not is encumbant upon the character of the person in question, some people just give up easier than others.

Darken
*smiles*


--------------------
~The first and most important step in cultivation of the wonderful mushroom, is the cultivation of patience for without it you doom yourself to failure~


Edited by Darkenshroom (04/24/06 09:08 PM)


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OfflineArchMagus
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Re: How many days after innoc. and nothing... do you give up? [Re: IGnosticAbhorI]
    #5552655 - 04/24/06 08:59 PM (17 years, 9 months ago)

Quote:

IGnosticAbhorI said:
After all, i'm SURE you've read their disclamier..."FOR STUDY ONLY"
-Gnostic




Do they say that for legal species also? I thought it was ok to cultivate legal ones.


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OfflineStainBlue
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Re: How many days after innoc. and nothing... do you give up? [Re: ArchMagus]
    #5552677 - 04/24/06 09:02 PM (17 years, 9 months ago)

From reading your post and your responses during this thread I would have to say that your main problem is most likely......Patience. When you give up on a jar or this hobby in general is all up to you and how patient you are willing to be. Anyone in this community will tell you patience is a virtue when it comes to our pasttime.

Growing a mushroom is a lot like watching the grass grow only 1000x more exciting.

Patience and persistance will come through in the end.


--------------------
Peace,
StainBlue


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OfflineArchMagus
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Re: How many days after innoc. and nothing... do you give up? [Re: StainBlue]
    #5558908 - 04/26/06 02:38 PM (17 years, 9 months ago)

Well it's been 5 days and no signs of anything... not even contamination.

I mean come on, at least let me see some green stuff or something

After pressure cooking the corn was in one big clump, does that mean it was overcooked?


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Offlinecaboomers
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Re: How many days after innoc. and nothing... do you give up? [Re: ArchMagus]
    #5558936 - 04/26/06 02:46 PM (17 years, 9 months ago)

i wouldve started over after 3 or so, start using lcs


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OfflineArchMagus
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Re: How many days after innoc. and nothing... do you give up? [Re: caboomers]
    #5558956 - 04/26/06 02:50 PM (17 years, 9 months ago)

Thanks can you point me to any good lc teks?


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OfflineSleepAid
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Re: How many days after innoc. and nothing... do you give up? [Re: ArchMagus]
    #5559008 - 04/26/06 03:01 PM (17 years, 9 months ago)

you did remove the tape form the holes, right?


--------------------
Signature this, ho


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OfflineArchMagus
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Re: How many days after innoc. and nothing... do you give up? [Re: SleepAid]
    #5564096 - 04/27/06 07:00 PM (17 years, 9 months ago)

I have tyvek as the top
- no metal lid, just the ring and the tyvek with micropore tape covering the tiny hole.

Is this ok? I don't have to uncover that hole do I?


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OfflineHongKongPhooey
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Re: How many days after innoc. and nothing... do you give up? [Re: ArchMagus]
    #5564337 - 04/27/06 07:59 PM (17 years, 9 months ago)

I just punch one hole in the top & stuff w/ polyfill, then i just mix 1tbsp of karo in w/ 6tbs of h2o pc it for 20mi then its ready to go... but just using tyvek for a top sounds interesting and easy. you can cover the injection hole w/ any tape. they lc doesnt really need much fae... the dam stuff is growing under water anyways


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Invisibleshymanta
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Re: How many days after innoc. and nothing... do you give up? [Re: ArchMagus]
    #5566074 - 04/28/06 06:31 AM (17 years, 9 months ago)

Here's some advice concerning this hobby. When you have a jar of something incubating, forget about it and read this website until you forget what species and strain you're growing.

As a noob, I confronted the same issues with patience. The way I solved it was to begin a new project that kept my mind off the jars already started. That way you've got something to keep your mind off it and if the first one fails, you've got another one going.

Don't give up. You'll be missing out on something good. The more you become bored with it, the more exciting it will become.


Edited by shymanta (05/15/06 07:09 PM)


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Offlinehyphae
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Re: How many days after innoc. and nothing... do you give up? [Re: shymanta]
    #5566083 - 04/28/06 06:43 AM (17 years, 9 months ago)

I've had them "pop" after close to three weeks and the myc was very vigorous so it was a pleasant suprise!


--------------------
Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal :wink:
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE

"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"


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OfflineArchMagus
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Re: How many days after innoc. and nothing... do you give up? [Re: HongKongPhooey]
    #5566516 - 04/28/06 10:24 AM (17 years, 9 months ago)

What are you using, just a pint sized jar? Would making your amounts bigger and using a qt. jar be bad idea... or just use same amounts in a qt. jar?

To everyone else too:
Thanks for all the advice guys


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OfflineArchMagus
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Re: How many days after innoc. and nothing... do you give up? [Re: ArchMagus]
    #5570473 - 04/29/06 02:13 PM (17 years, 8 months ago)

Well I am finally starting to see something, it is not white but a lighter colored grey.

Is it trash now?


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OfflineBPJohnny
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Re: How many days after innoc. and nothing... do you give up? [Re: ArchMagus]
    #5570702 - 04/29/06 03:30 PM (17 years, 8 months ago)

i dont know if anyone hit on this, you said you were using just tyvek

for a lid correct? I am almost certain that i have read somewhere that

doing that will dry out your substrate. if your substrate is too dry the

myc will grow slow if at all and if it does grow it will stall and

most likely never hit 100% definitely use a lid with a hole drilled or

punched in it.


Edited by BPJohnny (04/29/06 03:30 PM)


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OfflineArchMagus
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Re: How many days after innoc. and nothing... do you give up? [Re: BPJohnny]
    #5570721 - 04/29/06 03:34 PM (17 years, 8 months ago)

I heard thats only if you are not shaking the jars while its colonizing (ie. once at 25%, 50% 75%.. etc.) That and make sure you have verm mixed in with the grain... which I do.

My problem is, it's been over a week and I have seen NOTHING at all !! But now like I said... some light greyish colors.


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OfflineBPJohnny
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Re: How many days after innoc. and nothing... do you give up? [Re: ArchMagus]
    #5570731 - 04/29/06 03:38 PM (17 years, 8 months ago)

right but the tyvek allows the jars to "breathe" it just doesn't allow

contams through. so if your jars are "breathing" through a huge space

instead of a small hole the jars will dry out much faster and if you

took them out of the pc before completely cooled they will definitely

dry out very very quickly


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OfflineArchMagus
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Re: How many days after innoc. and nothing... do you give up? [Re: BPJohnny]
    #5570740 - 04/29/06 03:41 PM (17 years, 8 months ago)

Well with all the verm I have mixed in there, it still is plenty wet in there... I can see all the moisture on the sides of the glass.


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OfflineArchMagus
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Re: How many days after innoc. and nothing... do you give up? [Re: ArchMagus]
    #5570760 - 04/29/06 03:50 PM (17 years, 8 months ago)

Plus Reckless seems to have had no problem using just tyvek for the lid:
http://www.shroomtalk.com/mushroom/forum6/668.html

He also has this to say about it, a couple posts down in that thread:
"If you use the lid with the tyvek,your tyvek will melt to a mess."

Still doesn't explain why I didn't see anything at all... unless I just have to wait more.



Edited by ArchMagus (04/29/06 03:58 PM)


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OfflineBPJohnny
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Re: How many days after innoc. and nothing... do you give up? [Re: ArchMagus]
    #5570781 - 04/29/06 03:56 PM (17 years, 8 months ago)

OK well its just my 2 cents i just recommend that you use a lid in

future applications. i found some literature on the subject, in

The Mushroom Cultivator on page 46- "Using only filter discs on wide mouth jars is not recommended due to the excessive evaporation from the grain medium"-

and if you have grey growth you could have cobweb mold in there and if

you have one contam you could have other contams that make it seem wet

such as wet spot or wet bubble. however i have not seen your jars so i

really dont know what the moisture content is really like its just an

idea/suggestion


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OfflineArchMagus
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Re: How many days after innoc. and nothing... do you give up? [Re: BPJohnny]
    #5570818 - 04/29/06 04:07 PM (17 years, 8 months ago)

Thanks... how do you get around melting the tyvek though??


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OfflineBPJohnny
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Re: How many days after innoc. and nothing... do you give up? [Re: ArchMagus]
    #5570829 - 04/29/06 04:10 PM (17 years, 8 months ago)

melting the tyvek? i dont know anything about melting it. follow
6tango's (agar's) tek http://www.shroomery.org/index.php/par/23619


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OfflineArchMagus
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Re: How many days after innoc. and nothing... do you give up? [Re: ArchMagus]
    #5570848 - 04/29/06 04:16 PM (17 years, 8 months ago)

Quote:

ArchMagus said:
He also has this to say about it, a couple posts down in that thread:
"If you use the lid with the tyvek,your tyvek will melt to a mess."




Is that true??


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OfflineBPJohnny
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Re: How many days after innoc. and nothing... do you give up? [Re: ArchMagus]
    #5570864 - 04/29/06 04:21 PM (17 years, 8 months ago)

i just read the tek twice over i dont see that anywhere and anyway i use
a lid with tyvek and pc for 90 min @ 15 psi and haven't had any problems
with melting tyvek


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OfflineArchMagus
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Re: How many days after innoc. and nothing... do you give up? [Re: BPJohnny]
    #5570885 - 04/29/06 04:27 PM (17 years, 8 months ago)

Its about 19 posts down in the thread:
Quote:


Posted: Fri Mar 24th, 2006 05:04 am
It is nessasary for air exchange to take place in order for myc to grow,if the jar was sealed you would see little to no growth..On a brf cake your verm layer provides the same,filters and allows for gas exchange.If you use the lid with the tyvek,your tyvek will melt to a mess...noc thru the tyvek and patch hole with small pc of tape..

also if your jar is sealed tight b4 pc,it may explode..




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OfflineBPJohnny
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Re: How many days after innoc. and nothing... do you give up? [Re: ArchMagus]
    #5570988 - 04/29/06 04:50 PM (17 years, 8 months ago)

the link i gave you doesn't even have 19 posts, agar is an experienced
veteran here you can be pretty sure that he is right. and it wont seal
if you have a hole in the lid. i have used the tek and have had success
with out encountering melted tyvek.


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OfflineArchMagus
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Re: How many days after innoc. and nothing... do you give up? [Re: BPJohnny]
    #5571318 - 04/29/06 06:28 PM (17 years, 8 months ago)

I was talking about the link I gave you. I guess I should not listen to reckless anymore... he is full of shit?


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OfflineEquilibriuM
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Re: How many days after innoc. and nothing... do you give up? [Re: ArchMagus]
    #5571408 - 04/29/06 06:54 PM (17 years, 8 months ago)

no problems with tyvec melting here  :tunnel:


--------------------
HELP!!!!!!!!!


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OfflineBPJohnny
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Re: How many days after innoc. and nothing... do you give up? [Re: EquilibriuM]
    #5571414 - 04/29/06 06:55 PM (17 years, 8 months ago)

all i can say is that i know there are lots of people here who use lids
with tyvek and i use them too and i have never heard anyone talking
about or have i experienced melted tyvek. now as far as it working for
him, I'm not saying it CANT work his incubator was probably humid enough
that the substrate doesn't dry out.


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OfflineArchMagus
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Re: How many days after innoc. and nothing... do you give up? [Re: BPJohnny]
    #5581961 - 05/02/06 04:12 PM (17 years, 8 months ago)

Thanks for all the help ! I think I am done trying cracked corn... seems too hard to get the moisture content correct.


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