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Offlinewiggles
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Spores need to soak with agar?
    #5551882 - 04/24/06 06:12 PM (17 years, 9 months ago)

Hey all! I just recieved a lot of 20 potato dextrose agar petri dishes that I managed to get my hands on. However, I don't have any living fruitbodies at the moment. I DO however have 3 spore prints, and I'll be ordering another spore syringe within a week or so. This leads me to two questions:

At least from the teks I saw in the cultivation section, all of them deal with living fruitbodies. Is it possible to just insert dry spores into the agar? Should I rehydrate them first? I know agar contains a decent amount of water so I figured they should be fine rehydrating themselves, but I was hoping someone with more experience could let me know for sure.

Also, for the spore syringe, is it possible to just inject a small amount of the contents of the syringe (ca. .25 cc's or so) into the sterile agar? Would the added water hurt anything?

I'm mostly concerned about the prints as I'm not entirely sure if they're contam free and I'd like to make sure before I innoc anything. Thanks guys!


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OfflineCrackalack
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Re: Spores need to soak with agar? [Re: wiggles]
    #5551955 - 04/24/06 06:36 PM (17 years, 9 months ago)

I believe you use an inoculation loop and scrape the surface of the print and then move the inoculation loop across the agar and the spores are spread across the agar. http://www.williamsbrewing.com/images/product_images/Q22.gif thats a pic of an inoculation loop.


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Offlinewiggles
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Re: Spores need to soak with agar? [Re: Crackalack]
    #5552505 - 04/24/06 08:36 PM (17 years, 9 months ago)

Ok, where exactly can I find one of these? I've got a few friends who work in bio labs who can look and see if one might fall off of a delivery truck by chance... other than that.. should I check someplace like sigma?

edit:
Damn, I just checked sigma and all i saw were polystyrene loops. Anyone know if this material is autoclavable?


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You can turn your back on a person, but never turn your back on a drug, especially when its waving a razor sharp hunting knife in your eye.
Hunter S. Thompson


Edited by wiggles (04/24/06 08:52 PM)


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OfflineBPJohnny
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Re: Spores need to soak with agar? [Re: wiggles]
    #5552591 - 04/24/06 08:51 PM (17 years, 9 months ago)

I am pretty sure that at least one of the venders here has them i believe myco supply if you can not find them at any of the vendors pm me and i can give you another site that you can find them on


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OfflineBPJohnny
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Re: Spores need to soak with agar? [Re: BPJohnny]
    #5552615 - 04/24/06 08:55 PM (17 years, 9 months ago)



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Offlinewiggles
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Re: Spores need to soak with agar? [Re: BPJohnny]
    #5552788 - 04/24/06 09:19 PM (17 years, 9 months ago)

Sweet thank you so much! I've been browsing but I couldn't find what size was appropriate when working with spores. Thanks again :smile:


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You can turn your back on a person, but never turn your back on a drug, especially when its waving a razor sharp hunting knife in your eye.
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OfflineRogerRabbitM
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Re: Spores need to soak with agar? [Re: wiggles]
    #5553204 - 04/24/06 10:49 PM (17 years, 9 months ago)

You can make your own loop out of stainless steel wire available at hardware and craft stores. You just want something round like wire so it doesn't destroy the spores when you rub over them. You also need to make it out of a material you can heat with a flame red hot to sterilize. You can use a scalpel or exacto knife, but you'll destroy a lot more spores than a proper inoculation loop.

The spores will rehydrate once on the agar.
RR


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