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Offlinejonnyjonjonjon
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Alteration of BRF/Verm tek
    #5544179 - 04/22/06 01:46 PM (17 years, 9 months ago)

I was mixing up some substrate about 2 weeks ago but I decided to alter the ratio's a bit. Instead of putting in 2 verm/1 BRF/1 H20 I mixed up 2.5 verm/1 BRF/0.5 coco coir/1.5 water but for some reason not a single jar has colonized.

At first I thought the syringe might have been a dud but I made a honey water culture and its literally swarming with little white strands of mycellium.

Can I safely assume its a shitty substrate mixture or should I keep waiting for signs of colonization.

I should also add I brought the temp in my incubator down to around 77 for my casing trays. Maybe the spores are just taking extremely long to germinate because of the low temerature.


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Mother goose said to the swan "Is that PCP your cooking?" and the swan replied "Yes, yes it is.


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Offlineskeletor
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Re: Alteration of BRF/Verm tek [Re: jonnyjonjonjon]
    #5544293 - 04/22/06 02:31 PM (17 years, 9 months ago)

should germ in a week at that temp if not still before.

maybe your LC was bad. myc was that of something else.

i don't think that your substrate was the problem since its pretty dman close.

next time do a few normal PF cakes aswel as a controle.


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Offlinejonnyjonjonjon
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Re: Alteration of BRF/Verm tek [Re: skeletor]
    #5544351 - 04/22/06 02:47 PM (17 years, 9 months ago)

Yeah thats pretty strange. I actually did inoculate about 3 normal ratio jars the only thing I did different was put about .4 cc per jar to save spores.

I havent used my LC yet. The reason I find it strange is because the liquid culture is crawling with mycellium from the same syringe but the jars havent colonized.

Im going to inoculate about 2 of my alternated jars with my LC and use up the rest of the liquid culture on normal ratio jars to see what happens.

I was wondering if I can use liquid culture mycellium to inoculate more LC's? I dont see why I couldnt. Im running pretty low on money so I need to reproduce my spores any way I can and seeing as Im not advanced enough to use rye as substrate yet LC's seem like a good way to go.

I say im not advanced enough to grow with rye but I havent even tried it yet. Im going to get a bag of rye or birdseeds and make jars and inject them with the LC sometime soon.


--------------------
Mother goose said to the swan "Is that PCP your cooking?" and the swan replied "Yes, yes it is.


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Offlinejonnyjonjonjon
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Re: Alteration of BRF/Verm tek [Re: jonnyjonjonjon]
    #5544361 - 04/22/06 02:51 PM (17 years, 9 months ago)

I should also add I bought the syringe from high times and the bastards couldnt even tell me what strain it was. They said if its a dud to bring it back but how in the name of god can I prove it was a dud to them without incriminating myself and high times.


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Mother goose said to the swan "Is that PCP your cooking?" and the swan replied "Yes, yes it is.


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Offlineshroomballa
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Re: Alteration of BRF/Verm tek [Re: jonnyjonjonjon]
    #5544739 - 04/22/06 05:45 PM (17 years, 9 months ago)

well, if your substrate was undersaturated, the addition of your LC (i'm assuming ~4cc/jar?) could have caused the coir to expand to a point where your jar is PACKED with coir/verm. (This happened to me) after two weeks of no growth, I chopped one cake apart, and it was very very hard and compact....maybe just check one jar?
Also, you used a 3:1 verm+coir:BRF, as opposed to the normal 2:1 verm:brf, any reason why? I would this thie would eventually lead to less weight...


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Offlinejonnyjonjonjon
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Re: Alteration of BRF/Verm tek [Re: shroomballa]
    #5550835 - 04/24/06 11:36 AM (17 years, 9 months ago)

I accidentally froze my LC today. If I thaw it out is there any chance the mycellium will survive?


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Mother goose said to the swan "Is that PCP your cooking?" and the swan replied "Yes, yes it is.


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