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OfflineTylerneedshelp
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Registered: 09/16/05
Posts: 66
Last seen: 16 years, 3 months
mixing strains * 1
    #5543678 - 04/22/06 11:12 AM (17 years, 9 months ago)

Is it ok to mix different strains? Its possible that i mixed up my jars from the labels and i just spawned? them to horsepoop. If i have 2 or 3 strains mixed together will there be a problem?


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InvisibleDarkenshroom
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Re: mixing strains [Re: Tylerneedshelp]
    #5543702 - 04/22/06 11:19 AM (17 years, 9 months ago)

Accoring to some they will fight and it will be detrimental, accoring to other it wont matter and youd be hard pressed to tell the difference.

The first is the common consensus, the second is a statement from RogerRabbit that came up in a thread just a week or two ago...

Take from that what you will,
Darken
*smiles*


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Offlineskeletor
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Re: mixing strains [Re: Darkenshroom]
    #5543899 - 04/22/06 11:57 AM (17 years, 9 months ago)

they won't kill each other but dont' do it. remember a multispore inconulations is many strains in fact. but having several on one casing you won't know what is what. so its useless for printing if you care to keep track of what you are growing.

just do a few smaller casings.


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Get back to nature; hunting burgers and gathering fries.


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OfflineRogerRabbitM
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Re: mixing strains [Re: skeletor]
    #5544813 - 04/22/06 06:07 PM (17 years, 9 months ago)

I seriously doubt you'll notice any difference.  A multispore inoculation results in thousands of strains.  It really doesn't matter if some of those come from different prints with different names or not, since they're the same species.  Of topic sort of, but one of my best pinsets ever was when I deliberately mixed pans and cubes in the same jar.  Not just the same tray, but two different species in the very same grain jar.  Even shook the jar twice to mix them all up, and when it pinned, there were pan areas and cube areas, but EVERY square inch of the casing layer had half a dozen pins of one kind or the other.

I've just found the old adage of 'they'll fight with each other' to be incorrect.
RR


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InvisibleSimisu
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Re: mixing strains [Re: RogerRabbit]
    #5544841 - 04/22/06 06:18 PM (17 years, 9 months ago)

wow.. did you try to reproduce this and check it out?

maybe they're more motivated to pin/fruit when another spicies is around?


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OfflineBPJohnny
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Re: mixing strains [Re: Simisu]
    #5544948 - 04/22/06 06:56 PM (17 years, 9 months ago)

I have also experimented in this and my grow went just fine. I used

the pf tek and inoculated jars with several different types of

'strains' it was a while ago but I do remember that I used b+ and

amazonian there was a third but I forgot it. For scientific

information I will quote

The Mushroom Cultivator

"Two dikaryotik mycelial networks can also gro together, exchange genetic material and form a new strain. Such an encounter, where two hyphal systems fuse, is known as anastomosis. When two incompatible colonies of mycelia meet, a zone of inhibited growth frequently forms. On agar media, this zone of incompatibility is visible to the unaided eye."

Now correct me if I'm wrong but in ANY multi spore inoculation

there are many different strains so this process will happen

regardless if you mix different syringes or just use one.


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Offlineskeletor
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Re: mixing strains [Re: BPJohnny]
    #5544969 - 04/22/06 07:03 PM (17 years, 9 months ago)

as for RR's pan/cube casing maybe they're way of fighting is to see who can produce the most spores thereby repoducing more. this woudl mean creating fruits. id assume being different species they wouldnt' share teh myc network and therefor treat each other as competitors. of the scare recourse of nutients. once the jar was grown out they took sides and the all they could do waas build up and reproduce. something like what happends with playing teh board game ricks with a bunch of pussies who never attack and just sitting ther building up.
damn you risk!


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im sorry about your mother. She was a terrible attrative woman.

Get back to nature; hunting burgers and gathering fries.


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OfflineRogerRabbitM
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Re: mixing strains [Re: skeletor]
    #5545843 - 04/22/06 11:10 PM (17 years, 9 months ago)

Quote:

BPJohnny said:
The Mushroom Cultivator

"Two dikaryotik mycelial networks can also gro together, exchange genetic material and form a new strain. Such an encounter, where two hyphal systems fuse, is known as anastomosis. When two incompatible colonies of mycelia meet, a zone of inhibited growth frequently forms. On agar media, this zone of incompatibility is visible to the unaided eye."

Now correct me if I'm wrong but in ANY multi spore inoculation

there are many different strains so this process will happen

regardless if you mix different syringes or just use one.




To a certain extent, yes. They may fuse, and they may remain separate and fruit separate. When unlike mycelium are placed together on a petri dish, this can be observed. As Paul said, a line of inhibited growth forms between the two strains. This can be observed below. Both of these growths originated from the same sporeprint, but were isolated down to single sector isolated strains. These two isolates are not compatible, therefore a line of separation has formed between them.


Other times, after the zone of isolation has formed, a distinct third growth will emerge from it. This third growth as shown below emerging from the 12 o'clock position is a hybrid of the two mycelium, and is usually more vigorous than either parent.


When spores germinate and then pair up with compatable hyphae to form multiple strains that then fuse by anastomosis, the results can be seen in the picture below. The single sector at the bottom of the growth is steadly becoming more of the total as it assimilates the other strains into its network. If allowed to grow, it might have consolidated the entire dish into one strain or it may have ran up against an incompatible strain as seen in the pics above and stopped.

Holding a petri dish up to light and looking at it from the agar side is a good way to see the line of inihibition between the strains.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison


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Offlineskeletor
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Re: mixing strains [Re: RogerRabbit]
    #5545852 - 04/22/06 11:13 PM (17 years, 9 months ago)

ooooo curious informative post!


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im sorry about your mother. She was a terrible attrative woman.

Get back to nature; hunting burgers and gathering fries.


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OfflineZellaX
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Re: mixing strains [Re: RogerRabbit]
    #5545859 - 04/22/06 11:14 PM (17 years, 9 months ago)

Wow, those pics are awesome, Roger, thanks for posting.


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OfflineTheBlackCat
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Re: mixing strains [Re: RogerRabbit]
    #24412970 - 06/17/17 04:16 PM (6 years, 7 months ago)

I want to try this someday just for fun.


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Offlinemrmazdarx9
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Re: mixing strains [Re: TheBlackCat]
    #24412987 - 06/17/17 04:22 PM (6 years, 7 months ago)

:facepalm: 11 year old thread


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OfflineTheBlackCat
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Re: mixing strains [Re: mrmazdarx9]
    #24412989 - 06/17/17 04:23 PM (6 years, 7 months ago)

Hey you keep your dancing dick buffet to yourself.


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OfflineFungi4thisguy
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Registered: 03/08/23
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Re: mixing strains [Re: TheBlackCat]
    #28256099 - 03/31/23 12:06 PM (9 months, 24 days ago)

Love Aubrey plaza, I know this thread is old or maybe it isn't but I just seen it, Aubrey plaza and shrooms hmmm thanks for the visual, when I met her a few years back she was just as fuckin cool and gorgeous as she seems when you see her on tv and movies. I was lucky enough to share a corney vape pen of some butter nut cush... Hey don't judge it was cool back then but I was happy to smoke anything with her, wish she wasn't with her now husband maybe I was just in shock but I think she was into me 🤣


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OnlineScrewup
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Re: mixing strains [Re: Fungi4thisguy]
    #28256110 - 03/31/23 12:12 PM (9 months, 24 days ago)

Good bump bro super useful necro of a thread


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Invisiblemyc_ousin_vinny
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Re: mixing strains [Re: Screwup]
    #28256112 - 03/31/23 12:14 PM (9 months, 24 days ago)

:idigit:


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