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Shop: Mushroom-Hut Liquid Cultures   North Spore Injection Grain Bag

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OfflineBabo911
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re-sterlise Vacutainers
    #5542505 - 04/21/06 10:20 PM (17 years, 9 months ago)

Is it possible?


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InvisibleJaeger
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Registered: 10/01/05
Posts: 960
Re: re-sterlise Vacutainers [Re: Babo911]
    #5542516 - 04/21/06 10:23 PM (17 years, 9 months ago)

I have read agar saying it is.
Here is how I would think you could go about it.
Just pull out the plugs, put them in a mason jar, PC.  As they cool, the jar should seal and be sterile.
Move into a glovebox or a flowhood, and put the stoppers back in. Granted you will loose the vacuum in there, but they will still be usable.  When injecting, just release the plunger periodically to allow the displaced air to enter the syringe you are using.
Good luck :smile:


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OfflineBabo911
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Re: re-sterlise Vacutainers [Re: Jaeger]
    #5542522 - 04/21/06 10:26 PM (17 years, 9 months ago)

in the masson jar about how much water in say a pint?

And i had planed on using a sterial syringe and sucking the air out, i think it may work? I like keeping my mature LC in them :smile:


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InvisibleJaeger
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Re: re-sterlise Vacutainers [Re: Babo911]
    #5542533 - 04/21/06 10:31 PM (17 years, 9 months ago)

I would just use a dry mason jar.  The only reason I would recommend it is so that no contam'd air comes in contact with the opened vac's.
They will be in their own sterile environment until you can put them back together in a sterile area.
I really wouldn't try sucking out air :smile:  Just inject, let finger off plunger.  The air will enter the syringe, and repeat.  Make sense?

Again, this is only what makes sense to me, I have not tried it, but dont see why it wouldn't work :smile:


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OfflineBabo911
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Re: re-sterlise Vacutainers [Re: Jaeger]
    #5542542 - 04/21/06 10:35 PM (17 years, 9 months ago)

yea, now it makes sense. Thanks :smile:


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OfflineHippieChick
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Re: re-sterlise Vacutainers [Re: Babo911]
    #5542633 - 04/21/06 11:06 PM (17 years, 9 months ago)

They're cheap . Just buy 100  :thumbup:

Peace,Love,Happiness and Harmony
:heart: Hippie Chick  :mushroom2:


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OfflineBabo911
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Re: re-sterlise Vacutainers [Re: HippieChick]
    #5542664 - 04/21/06 11:18 PM (17 years, 9 months ago)

I get them for free :smile: I just like to reuse what i can


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