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Shop: Original Sensible Seeds Bulk Cannabis Seeds   Mushroom-Hut Liquid Cultures   North Spore Bulk Substrate, Injection Grain Bag

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Offlinedeva1
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Is my LC done for?
    #5539511 - 04/20/06 11:01 PM (17 years, 9 months ago)

So I started up an Equadorian LC from agar that I won from a photo-contest last fall. First LC I've done so my technique wasn't that great.....had a little trouble getting all the LC out of the vacutainer, so I removed the plug and sucked it all up in a large, sterile 30 cc syringe. Guess I screwed up my sterility there?? Squirted most of the syringe into my Karo tek pint, and knocked up 2 quarts of rye just for kicks. At 10 days old the LC is looking good I think...... lots of ropy looking sea-creature clumps floating around. Most all of the clumps are milky white, but on some there appears to be a very thin light brown thread running through the clump....not sure if this is contam or what?

So......for the 2 rye jars shot up with LC I got green mold going. These 2 jars were part of a larger set which were all prepared the same way. I knocked up other jars in the set with a separate GT culture and they are all growing nicely. So, should I suspect that since that the 2 jars knocked with the LC are contamed that my LC is contamed as well? I would really like to grow out this EQ variety, but I don't want to waste time prepping 10 grain jars to just inoculate them with a ill LC culture. Is there anyway to check if the LC is spoiled without prepping and shooting up more jars?


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Offlinedeva1
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Re: Is my LC done for? [Re: deva1]
    #5541107 - 04/21/06 01:38 PM (17 years, 9 months ago)

Clarification: the first statement of starting up an Equadorian LC from 'agar' meaning the grower extrordinaire Agar, not the substrate. Just trying not to confuse anyone-


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Offlineshroomsynergy
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Re: Is my LC done for? [Re: deva1]
    #5544530 - 04/22/06 04:18 PM (17 years, 9 months ago)

oh, how mean the green monster. sounds like its contaminated to me, but i'm no pro. get a second opinion.


....and a third, and fourth, and....well, you know.

good luck.


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Offlinedeva1
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Re: Is my LC done for? [Re: shroomsynergy]
    #5552791 - 04/24/06 09:19 PM (17 years, 9 months ago)

Any takers on this question???? If in doubt.......throw it out??


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InvisibleDarkenshroom
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Re: Is my LC done for? [Re: deva1]
    #5552846 - 04/24/06 09:26 PM (17 years, 9 months ago)

I had an LC do that, everything I inoc'd with it got mean green fast. I tossed it, my guess is your lc is contam'd and its toast.

Darken
*smiles*


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~The first and most important step in cultivation of the wonderful mushroom, is the cultivation of patience for without it you doom yourself to failure~


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InvisibleCureCat
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Re: Is my LC done for? [Re: Darkenshroom]
    #5553578 - 04/25/06 12:43 AM (17 years, 9 months ago)

Picture would help. Sounds like contam. I hear if ya do outdoor grows theres less risk of contamination. That sounds weird, but with so many options for the molds to grow without competition, and the more harsh outdoor habitat, it seems reasonable. I mean, they grow in the wild anyway. Id say if yer gonna throw it out, then you might ass well inoculate it into some manure and toss it around yer yard or in one spot. not sure the procedure, you might want to do some more looking up on outdoor growing and see if it is a viable alternative or not. Good luck.


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Offlinedeva1
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Re: Is my LC done for? [Re: CureCat]
    #5568624 - 04/28/06 09:35 PM (17 years, 9 months ago)

Thanks - will definitely toss it into a well saturated patty outside......and see what happens


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