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Offlinealchemike
Stranger
Registered: 10/02/05
Posts: 43
Last seen: 1 year, 2 months
peroxide eliminates sterilization???
    #5539432 - 04/20/06 10:32 PM (17 years, 9 months ago)

is that what this guy is saying???

http://members.aol.com/PeroxyMan/

is it possible to grow cubensis in an essentially non-sterile manner??? ie...no pressure cooker...no bioling water...just mix up the substrate and inoculate...

crazy???

o)<

mike


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Offlinemikeownow
Humungus fungus

Registered: 09/01/05
Posts: 2,856
Loc: WA,USA
Last seen: 17 years, 3 months
Re: peroxide eliminates sterilization??? [Re: alchemike]
    #5539438 - 04/20/06 10:35 PM (17 years, 9 months ago)

If peroxide fights of contams,,,, then lets say You inoculate a jar that way and then mycelium grows so if mycelium can grow then so can other molds? doesnt seem like it would work


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No statements made in any post or message by myself should be construed to mean that I am now, or have ever been, participating in or considering participation in any activities in violation of any local, state, or federal laws. All posts are works of fiction.


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Offlinemogur
regnartS

Registered: 11/15/05
Posts: 322
Loc: Puget Sound
Last seen: 11 years, 2 months
Re: peroxide eliminates sterilization??? [Re: mikeownow]
    #5539850 - 04/21/06 12:56 AM (17 years, 9 months ago)

It can help, but it doesn't do away with sterilization. Established mycelium can resist peroxide by producing an enzyme that breaks it down, and thereby protects itself. Bacteria doesn't have the same ability, so H2O2 can be effective in keeping contams down enough to let mycelium get the upper hand. You're not going to be able to just slosh some into your culture and give up on sterile technique, however. First, it will kill spores since they don't have a defense mechanism against it, like mycelium does. Also, most complex organic molecules will break down peroxide, so you have to PC the substrate to denature them and prevent them from immediately inactivating the peroxide. It's a method that is tried and true, but some find it a help, while others think it is just extra work. You need to read up on the method promoted by Dr. R. Wayne in the link you posted, to find out if it is worth it to you.


Edited by mogur (04/21/06 12:59 AM)


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Invisiblecreamcorn
mad scientist
Male User Gallery

Registered: 03/13/06
Posts: 2,962
Trusted Cultivator
Re: peroxide eliminates sterilization??? [Re: mogur]
    #5540247 - 04/21/06 07:42 AM (17 years, 9 months ago)

a while ago i tried a "blank" pf mix jar with water and h2o2 and no sterilizing... worked great for the 10 days or so, no contams, then the h2o2 apparently had broken down to the point where contam spores that survived finally were able to germinate, it lit up all sorts of greys and whites and greens real quick after :-)

h2o2 definitely has its uses but a replacement for intitial sterilization it is not


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