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OfflineBowevil
newbie
Registered: 01/25/02
Posts: 25
Loc: Netherlands
Last seen: 14 years, 8 months
starting from print
    #553361 - 02/16/02 07:34 AM (14 years, 9 months ago)

I wound up getting a spore print from mush mush, whilst making my syringes, I couldn't seem to scrape the spores off quickly, so I threw the entire print and the paper in the small jar (40ml for 3 syringes) I hope that the paper was sterile enough! did I seriously screw up, should I even try to innoculate? mistakes happen even stupid ones I guess, can always try again!


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OfflineAnnoA
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Registered: 06/18/99
Posts: 24,121
Loc: my room
Last seen: 2 months, 15 days
Re: starting from print [Re: Bowevil]
    #553401 - 02/16/02 10:29 AM (14 years, 9 months ago)

The paper is quite sterile so I would try inoculating with the syringes.



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OfflineAzure
old hand
Registered: 01/01/99
Posts: 469
Loc: California, USA
Last seen: 14 years, 7 months
Re: starting from print [Re: Anno]
    #553454 - 02/16/02 12:20 PM (14 years, 9 months ago)

If you touched the print, it's probably contaminated. However, if you use the solution fast enough, you'll have a smaller chance of that contamination ruining your experiment. There will be less bacteria or mycelium from a contaminant to compete against.


Edited by Azure (02/16/02 12:22 PM)


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Anonymous

Re: starting from print [Re: Bowevil]
    #553466 - 02/16/02 12:41 PM (14 years, 9 months ago)

The inside of the paper was surely sterile but the outside was likely handled by both you and the vendor and stands a good chance of being contaminated.....I would try to avoid dropping it in next time.

In the meantime, good luck with what you got.


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InvisibleNighted

Registered: 11/10/01
Posts: 564
Loc: U.S.A.
Re: starting from print [Re: Bowevil]
    #553479 - 02/16/02 01:02 PM (14 years, 9 months ago)

Scraping spores is easy when using the right tool. I use an X-Acto handle with the following blade:



It's razor sharp and its curved design makes it perfect for easily removing spores from glass, foil, and card stock. X-Acto makes every blade that you could possibly need for mushroom cultivation, better than anything Fungi Perfecti sells, that's for sure [IMNSHO]!

Find out more at: www.x-actoblades.com


--------------------

Freedom defined is freedom denied.


Edited by Nighted (02/16/02 01:04 PM)


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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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