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Offline12bodies
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Registered: 04/18/06
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decreasing colonization time
    #5529961 - 04/18/06 03:39 PM (17 years, 9 months ago)

Time is of the essence and cakes have taken pretty long to colonize in the past (approx 1.5 months). Previously the temperatures had been not so consistent or warm enough.

If one is looking to decrease the amount of time for one half-pint jar of vermiculite and brownrice flour to colonize fully, what are some techniques to cut down on the colonization time?

Plus, what is the minimum time, under ideal conditions, to colonize a half pint jar?

Thanks for the inputtttt!


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Offlineskeletor
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Re: decreasing colonization time [Re: 12bodies]
    #5529972 - 04/18/06 03:42 PM (17 years, 9 months ago)

swtich to grain. then you can shake the jars.
other then that make your mix very airy.
add some poo into your mix. and maybe grains. more of a potency thing i guess but i find my poo cakes to colonize very fast adn be very airy.


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OfflineESanceOfCyan
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Re: decreasing colonization time [Re: skeletor]
    #5530030 - 04/18/06 03:55 PM (17 years, 9 months ago)

BRF takes a long time to colonize,  but should take no longer than 30 days and can get done in 14days:shrug:(give or take).
Grains are much faster colonizing in around 10 days.

The only way to "cut down time" is to have them at optimal conditions, meaning: water content, temperature, gas exchange, and as little light as possible.


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OfflineYefet2000
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Re: decreasing colonization time [Re: ESanceOfCyan]
    #5530120 - 04/18/06 04:25 PM (17 years, 9 months ago)

Try TiT incubator, worth every penny..
and works well with PF.


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Me fail english ? Thats unpossible...


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OfflineRemainRandom50
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Re: decreasing colonization time [Re: Yefet2000]
    #5530163 - 04/18/06 04:40 PM (17 years, 9 months ago)

Quote:

Yefet2000 said:
Try TiT incubator, worth every penny..
and works well with PF.




true statement.
but also , i think you should defiently switch to a grain substrate if your looking for bulk.


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At times I get consumed by my everyday life and will leave the Shroomery. Yet, every time drugs come falling into my life for fun.....I always think about the Shroomery and then I'm back!


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OfflineMycomyth
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Re: decreasing colonization time [Re: Yefet2000]
    #5530181 - 04/18/06 04:45 PM (17 years, 9 months ago)

Since no one else said it... I would make an LC if you have some spores left. If not, when you have a jar at 100%, inject 10cc of sterile water into the jar (without opening it). Swirl for a minute, and suck the water back up. Inject this into an already sterilized and cooled LC.

The last Quinoa (PF tek, different flour) jars I hit with 4cc of LC are 80-90% (visible surface) at 10 days. Rampant growth at all 4 points and the entire bottom started at day 2.

Don't forget to incubate!

Myc


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Wave upon wave of demented avengers marched cheerfully out of obscurity into the dream.


Edited by Mycomyth (04/18/06 04:50 PM)


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Offline12bodies
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Re: decreasing colonization time [Re: Mycomyth]
    #5530219 - 04/18/06 05:01 PM (17 years, 9 months ago)

how about double injecting? would this be worth it in the long run for bulk?


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OfflineMycomyth
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Re: decreasing colonization time [Re: 12bodies]
    #5530255 - 04/18/06 05:10 PM (17 years, 9 months ago)

Actually would probably be a waste. Grain goes fast enough on its own.

M


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Wave upon wave of demented avengers marched cheerfully out of obscurity into the dream.


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Offlinehyphae
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Re: decreasing colonization time [Re: ESanceOfCyan]
    #5530843 - 04/18/06 08:05 PM (17 years, 9 months ago)

Quote:

ESanceOfCyan said:
The only way to "cut down time" is to have them at optimal conditions, meaning: water content, temperature, gas exchange, and as little light as possible.



Well put!


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Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal :wink:
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE

"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"


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Invisiblemungojerry
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Re: decreasing colonization time [Re: hyphae]
    #5531027 - 04/18/06 08:43 PM (17 years, 9 months ago)

what saved a lot of time for me was to inoculate between the glass and the substrate as opposed to the middle of the cake

ps ive had a cake fully colonize in a little over a week once the trick was to use a whole bunch of spore solution i take prints from ALL of my mushies!


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OfflineBPJohnny
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Re: decreasing colonization time [Re: mungojerry]
    #5531254 - 04/18/06 09:24 PM (17 years, 9 months ago)

Quote:

mungojerry said:
what saved a lot of time for me was to inoculate between the glass and the substrate as opposed to the middle of the cake

ps ive had a cake fully colonize in a little over a week once the trick was to use a whole bunch of spore solution i take prints from ALL of my mushies!




the problem with using alot of spore solution is that your adding more

water to your jar messing up the water ratio


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Offlinehyphae
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Re: decreasing colonization time [Re: BPJohnny]
    #5532497 - 04/19/06 06:07 AM (17 years, 9 months ago)

Quote:

BPJohnny said:
Quote:

mungojerry said:
what saved a lot of time for me was to inoculate between the glass and the substrate as opposed to the middle of the cake

ps ive had a cake fully colonize in a little over a week once the trick was to use a whole bunch of spore solution i take prints from ALL of my mushies!




the problem with using alot of spore solution is that your adding more

water to your jar messing up the water ratio



Which can definitely be a problem.


--------------------
Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal :wink:
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE

"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"


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Offlineblackout
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Re: decreasing colonization time [Re: hyphae]
    #5532507 - 04/19/06 06:16 AM (17 years, 9 months ago)

Water content can be a problem, so you can leave your jars calculatedly dry on purpose. If you normally add 60ml water to a jar and 1cc of spore solution then add 51ml water and you can add 10ml LC.

These jars are at day 1 200g of dry wheat grain each and water.

30ml LC each and after 3 days they look like this



Another large jar. 1.25kg dry wheat & water


inoculated with 195ml LC. This brought it to approximately 2.6kg wet, which is 108ml water per 100g grain.

4 days later looks like this



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OfflineAkamatsu
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Re: decreasing colonization time [Re: hyphae]
    #5532512 - 04/19/06 06:24 AM (17 years, 9 months ago)

Use a LC. I had some 1/2 pint BRF jars inoculated with 4cc's of KSSS LC made with honey and it only took 5 days for 100% colonisation.


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InvisibleFooMan
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Re: decreasing colonization time [Re: 12bodies]
    #5532609 - 04/19/06 07:19 AM (17 years, 9 months ago)

Back to your original questions:

Quote:

12bodies said:
If one is looking to decrease the amount of time for one half-pint jar of vermiculite and brownrice flour to colonize fully, what are some techniques to cut down on the colonization time?





- Do not pack the substrate down at all in the jars. Keep it loose and fluffy
- Use coarse verm
- Follow the 2:1:1 ratio verm:BRF:H2O
- If you grind your own BRF, grind it so that it's a little coarser, not real fine
- incubate in the mid to low 80's (F)
- Use a little more spore solution or a more concentrated spore solution
- Inoculate with an LC
- Inoculate with a more aggressive strain: Cambodian, Brazillian, etc.
- Take the tape off of your inoculation holes, but leave the foil on. You can remove the foil also, but I like to wait until the jar is 30+% colonized. The less colonized it is, the more likely you are to get contams. If you are incubating in a really sterile environment, I wouldn't worry about foil or tape.

There are probably a few more ways, but that's what I come up with off the top of my head. IME, exposing the jars to light doesn't effect myc growth at all, but can induce premature pinning.

Quote:


Plus, what is the minimum time, under ideal conditions, to colonize a half pint jar?





2 weeks is about the fastest I've done myself. Using an LC dramatically speeds things up.


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Offlinetonyperez420
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Re: decreasing colonization time [Re: FooMan]
    #5532614 - 04/19/06 07:23 AM (17 years, 9 months ago)

LC sounds like the shit!

Can somebody shoot me some links of good/simple LC teks?


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InvisibleFooMan
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Re: decreasing colonization time [Re: tonyperez420]
    #5532618 - 04/19/06 07:25 AM (17 years, 9 months ago)

Mix 4-5 cc's of light Karo with 90-100cc's of water in a jar. PC at 15psi for 20 minutes. Let cool. Inoculate with 2-3 cc's spore solution. Wait.


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OfflineAkamatsu
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Re: decreasing colonization time [Re: tonyperez420]
    #5532619 - 04/19/06 07:26 AM (17 years, 9 months ago)



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InvisibleFooMan
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Re: decreasing colonization time [Re: Akamatsu]
    #5532624 - 04/19/06 07:29 AM (17 years, 9 months ago)

I thought my explanation was pretty beginner friendly! :wink:


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Quick WBS Prep


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Offlinetonyperez420
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Re: decreasing colonization time [Re: FooMan]
    #5532653 - 04/19/06 07:47 AM (17 years, 9 months ago)

thanx,

foomanshroom, I will give that a shot...

where may I purchase light karo?

How long do I have to wait after inoculation?
I am assuming I should treat it as a regular grain jar? 80F no light?


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