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OfflineAnthony707
Shroomsville
Registered: 03/14/06
Posts: 150
Last seen: 14 years, 10 months
no signs of anything PLEASE HELP
    #5525732 - 04/17/06 04:38 PM (17 years, 9 months ago)

im doing the pf tek and i inoculated 5 days ago i dont see any signs of germinization yet.. is this normal i thought you would see some signs between 3-5 days.

also if i had contams would they be there by now or can they come at any time.

Also i did pressure cook my jars

thanks for any feedback you can give me


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Invisiblemusher_420
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Registered: 08/01/05
Posts: 2,691
Re: no signs of anything PLEASE HELP [Re: Anthony707]
    #5525752 - 04/17/06 04:42 PM (17 years, 9 months ago)

Whenever I have experienced mold contamination it has always been noticeable very early, about the same time as I see mycelium growing or even before.

I have had bacterial contamination after my jars were almost fully colonized. But I have only experienced bacterial contamination with WBS jars, not PF jars.


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OfflineBPJohnny
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Re: no signs of anything PLEASE HELP [Re: Anthony707]
    #5525753 - 04/17/06 04:43 PM (17 years, 9 months ago)

what temperature are you incubating at? if your at around 84F you should see something in a few days if the temp is lower it may take a bit longer my jars are about six days old at 84-86F


Edited by BPJohnny (04/17/06 04:44 PM)


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OfflinefreshlyPLUMP
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Re: no signs of anything PLEASE HELP [Re: musher_420]
    #5525760 - 04/17/06 04:44 PM (17 years, 9 months ago)

sometimes it takes a little bit longer, what strain are you using?


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OfflineAnthony707
Shroomsville
Registered: 03/14/06
Posts: 150
Last seen: 14 years, 10 months
Re: no signs of anything PLEASE HELP [Re: freshlyPLUMP]
    #5525787 - 04/17/06 04:49 PM (17 years, 9 months ago)

im using B+ and cambodian 3 of each in 1/2 pint jars. im incubating at around 80 degress

if i pressure cooked i SHOULD be ok as far as contams though right??


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OfflineMycomyth
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Re: no signs of anything PLEASE HELP [Re: Anthony707]
    #5525807 - 04/17/06 04:52 PM (17 years, 9 months ago)

Jars can sometimes take up to 2 weeks to pop. Give them some time, if things are right...they WILL come. I gained alot of patience keeping reef tanks for 7 years, and it was easier for me not to be so antsy.

Contams can come at any time, but if you haven't seen any after two weeks, you should be golden. Molds are easy to spot. Bacteria, on the other hand, can be more difficult to diagnose, but usually stinks to high heaven. If you PCd properly, and have a relatively clean incubator, chances are your jars would never contam if just left alone. I have a couple that have been sitting on the shelf uninnoculated for ~6 weeks, and they're still as clean as the day I put them there.

Patience truly is a virtue, especially in this endeavor.

HTH,
Myc


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InvisibleRoadkillM
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Re: no signs of anything PLEASE HELP [Re: Anthony707]
    #5525809 - 04/17/06 04:52 PM (17 years, 9 months ago)

Quote:

Anthony707 said:

if i pressure cooked i SHOULD be ok as far as contams though right??




you can introduce a contam to the sterile jars when you inoculate them.



not everyone gets spores to germinate fast...

wait a little longer and the mycelium might start up.


tc


--------------------
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OfflineAnthony707
Shroomsville
Registered: 03/14/06
Posts: 150
Last seen: 14 years, 10 months
Re: no signs of anything PLEASE HELP [Re: Roadkill]
    #5525822 - 04/17/06 04:55 PM (17 years, 9 months ago)

i know i know i just have to realize that it cant happen over night and im going to have to wait thats all there is to it.

thanks


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Offlinesatori85
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Re: no signs of anything PLEASE HELP [Re: Anthony707]
    #5525883 - 04/17/06 05:11 PM (17 years, 9 months ago)

I had 5 jars once that didnt go for weeks. Other jars i had made after went and were ready. I though tthe syringe was a fake and was super pissed at the supplier (didnt send anything but felt like a fool lol). Well sure enough a month later all of a sudden whit stuff started popping up in 5-6 different places. THe temp had been like... 70 and when it got a LITTTTLE bit hotter, like 74 it started. So for some reason those spores didnt like the room temp in that room in the start. Patience.


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Invisiblecreamcorn
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Registered: 03/13/06
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Re: no signs of anything PLEASE HELP [Re: satori85]
    #5526201 - 04/17/06 06:21 PM (17 years, 9 months ago)

did you let your jars cool? i've noticed you posting questions just about every step along the way, i looked at your history and you were asking about how to prepare your jars at noon, and by 6PM you were asking questions about how to use a syringe. Assuming it took a little while to finish up your jars, and at least an hour for the PC process, that doesn't leave very much time for them to have cooled down in between. It is true that it can take "longer than normal" for them to germinate so I hope for your sake that's all it is... injecting spores into a warm jar is a pretty good way to kill them though. You really should see signs about 95% of the time in 3-5 days at at least some of the inoculation points, but of course if you're new to it you might not be able to recognize those signs as quickly, so keep your fingers crossed!

About the contams, its been long enough that bacteria certainly would have kicked in, as long as the jars don't have an obvious smell different than the day you took them out of the PC you're probably fine there.


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OfflineRemainRandom50
Do You Need ToKnow Me?
Registered: 01/15/06
Posts: 1,695
Last seen: 14 years, 9 months
Re: no signs of anything PLEASE HELP [Re: creamcorn]
    #5526237 - 04/17/06 06:31 PM (17 years, 9 months ago)

PF usaulyl takes about 1-2 weeks to fully colonize with my expierence, and WBS about a week.


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OfflineAnthony707
Shroomsville
Registered: 03/14/06
Posts: 150
Last seen: 14 years, 10 months
Re: no signs of anything PLEASE HELP [Re: creamcorn]
    #5526389 - 04/17/06 07:14 PM (17 years, 9 months ago)

hey creamcorn yes i definealy let the jars cool down for about 9-10 hours they were very cool. Also i cant smell the jars i have the foil on still... should i take it off?????


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Invisibleboomerman
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Registered: 02/21/06
Posts: 294
Re: no signs of anything PLEASE HELP [Re: Anthony707]
    #5526579 - 04/17/06 07:49 PM (17 years, 9 months ago)

it took my b+ 1 and 1/2 week to even start to show signs of anything


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OfflineAnthony707
Shroomsville
Registered: 03/14/06
Posts: 150
Last seen: 14 years, 10 months
Re: no signs of anything PLEASE HELP [Re: boomerman]
    #5526590 - 04/17/06 07:51 PM (17 years, 9 months ago)

ok that makes me feel a little better


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Invisiblecreamcorn
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Re: no signs of anything PLEASE HELP [Re: Anthony707]
    #5527033 - 04/17/06 09:18 PM (17 years, 9 months ago)

taking the foil off and leaving your holes exposed is fine if you've got your layer of dry verm on top like pf tek/mmgg suggests. some folks like to wait until part way into colonization before doing so. you'll want to do it for sure when you get up over 50% to encourage gas exchange. either way it won't hurt (again assuming the dry verm layer) to take it off for a minute and give it a sniff. if its bacteria it will be obvious. if your jars are too wet, taking the foil off will allow little bits of moisture to evaporate as well, which could balance them back out. if you don't see something soon you might want to pull it off half the jars as an experiment.

bummer its taking so long... i can see some B+'s here in 1/2pint PF jars (used diluted coffee instead of plain water) that were shot up from a liquid culture on 4/12 that are already about 50%... not that i suggest you try these things just yet til you got the basics down, but if you have spores left you might want to start a liquid culture to see if anything grows in there, its much more obvious when it germinates and will let you see if your spores are viable first off, and second off will yield enough liquid to refill your syringe many times over later down the road should you need to try again. good luck.


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