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Invisiblestrain
journeyman
Registered: 01/29/02
Posts: 95
PC Sterialization
    #552444 - 02/15/02 11:07 AM (22 years, 2 months ago)

I am trying Azurepowers superfast colinization recipe and i was wonder how long to Pressure cook the jars? Will an hour be fine?

The mix is 1/8 cup quinoa, 1/8 cup whole grain rye flour, 1/4 cup vermiculite.


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Keep on dreaming, becauses when you stop dreeming its time to die.
-Shannon Hoon RIP

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Invisiblephrozendata
Carpal Tunnel

Folding@home Statistics
Registered: 04/23/00
Posts: 5,015
Re: PC Sterialization [Re: strain]
    #552456 - 02/15/02 11:14 AM (22 years, 2 months ago)

One hour at 15PSI seems safe. If you want to be super careful let the jars sit out for 12-24 hours before pressure cooking. This allows any bacterial (or other) endospores to germinate and be killed by the sterilization process. It is not necessary but may help if you experience a lot of bacterial contaminations.


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"There's only one corner of the universe you can be certain of improving and that's your own self. So you have to begin there, not
outside, not on other people" - Aldous Huxley

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Invisiblestrain
journeyman
Registered: 01/29/02
Posts: 95
Re: PC Sterialization [Re: phrozendata]
    #552481 - 02/15/02 11:34 AM (22 years, 2 months ago)

I read that suggestion somewhere, I thought it was only if your using whole substrate not a powder, or is it in all substrates?


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Keep on dreaming, becauses when you stop dreeming its time to die.
-Shannon Hoon RIP

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OfflineSeussA
Error: divide byzero

Folding@home Statistics
Registered: 04/27/01
Posts: 23,480
Loc: Caribbean
Last seen: 2 months, 5 days
Re: PC Sterialization [Re: strain]
    #552634 - 02/15/02 01:34 PM (22 years, 2 months ago)

Its a general suggestion for anybody that is having trouble with contamination regardless of substrates. Spores can be in flours as easily as whole grains.

I don't know why, but here is my guess. People that are using whole grains are generally a bit more experienced and using more substrate at a time. More substrate equates to a larger initial endospore load. Larger spore loads mean more chances of contamination. To help tip the odds back into the bulk growers favor, presoaking is used.

There is probably a more scientific reason, but in the end who really cares. If you are having problems, presoak. If your not having problems, why change? The way I look at it, one day lost to presoaking is time well spent if it helps to protects two months worth of investment.


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Just another spore in the wind.

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