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OfflineAlKaBonGG
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Registered: 04/09/06
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Last seen: 18 years, 11 days
A li'l help with aquiring ingrediants for the medium required for Gottliebs extract tek please?
    #5523967 - 04/17/06 09:49 AM (18 years, 1 month ago)

Hey hello everybody please squse my spellin :grin:!  I gotta holda Gottliebs Psilocybib Production and I tract down the agar and I got a line on some yeast and malt extract but I still gotta get the dextrose,Potassium Phosphate Dibasic and calcium carbonate.  Dose anyone elce do this extraction?  I found the yeast extract,malt extract and agar at a couple of health food stores.  If anyone elce dose this extraction could ya let me know where ya get your dextrose,potasium phosphate dibasic and calcium carbonate?  I read the book over alot and I got it on hand so Im shure Im gonna get it rite, I know Im a nube but Im prety good at stuff like this. Could someone tell me otc sources for these itims?  Im shure I could fallow all the steps in the book dillagently but I just gotta get the stuff together :smile:!  Could ya's tell me kinda what the stuff is too so I wont look stupid if anyone asks me anything?  If I dont have to Id rather not just come out and say Im tryinna do a Psilocybin extraction from some mycellim :crazy2:!  Please ignore my nubizzim and help me out by tellin me where I can get this stuff, I know that if I could just find an otc scorce for the potassiun phosphate dibasic,calcium carbonate and dextrose I can shurly fallow the directions in the book to a T and come up with the specified results.  Please help a nube with an advanced project in spite of his inexperiance. Il post pics and finished results when Im done :smile:!

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Invisiblemycofile
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Re: A li'l help with aquiring ingrediants for the medium required for Gottliebs extract tek please? [Re: AlKaBonGG]
    #5524626 - 04/17/06 12:08 PM (18 years, 1 month ago)

Gottleibs book is whack. It's a pipe dream to think psilo production is that simple in liquid media. You'll have to do a LOT more research than what was in that book.

Start with small trial runs to make sure it's working before you follow his suggestion of trying to make millions of doses.

but here are some answers:
dextrose is at the brewing suppliers, they may have it labled as corn sugar.
calcium carbonate can be bought at the same place, or ordered from sporeworks.

Still, you won't get the results you are expecting regardless of how well you follow directions.


--------------------
"From a certain point of view"
-Jedi Master Obi Wan Kenobi

PM me with any cultivation questions.

I just looked at my profile and realized I had a website at one point in time on geocities, it's not there anymore and I have no idea what I had on it. Anybody remember my website from several years aga? PM if so please.

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Invisibleagar
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Re: A li'l help with aquiring ingrediants for the medium required for Gottliebs extract tek please? [Re: mycofile]
    #5524731 - 04/17/06 12:35 PM (18 years, 1 month ago)

Mycofile is on the money, when he says this tek is WHACK.
It's BS.


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OfflineAlKaBonGG
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Registered: 04/09/06
Posts: 24
Last seen: 18 years, 11 days
Re: A li'l help with aquiring ingrediants for the medium required for Gottliebs extract tek please? [Re: agar]
    #5525715 - 04/17/06 04:35 PM (18 years, 1 month ago)

Hey Mycofile :smile2:!  Hey Agar :smile2:!  Good lookin out!  Im not really tryin to do an upscale op or anything. I just got a li'l time on my hands and found this book and it looks like fun!  Even if I get one dose per quart jar I'l be satisfied.  :crazy2:  The book seems prety straightforward in its directions and it seems like once I get alla the stuff I can start.  I figure once I get everything I need Il make however many jars I can with the bigest pot we got and like I said, Im not lookin to turn a profit so I dont think I'l be disapointed with ANY results:naughty:!  This just realy aughta be fun!  Even if I wind up with half the crystals the book sugests , or even less, Il be happy!  Where do yous think the flaws are in Gottliebs tek?  Like, is it in his extract tek or do ya's think that the stuff just wont grow the way he sugests?  Any improvement sugestions?  Thanks for any helpful sugestions anyone might have :smile:!

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Invisiblecreamcorn
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Re: A li'l help with aquiring ingrediants for the medium required for Gottliebs extract tek please? [Re: AlKaBonGG]
    #5526537 - 04/17/06 07:43 PM (18 years, 1 month ago)

i think the biggest flaw is that there simply isn't much actives in mycellium.  supposedly if you time it just right you have a chance of catching a slightly higher concentration, although even if that's the case i'm still skeptical of a magic window of opportunity you should harvest it, and how to know when that is.  you will end up with less than what the book suggests by a long shot.  in fact you very likely will end up with nothing at all.  even using his impossibly unrealistic numbers, he only claims 2-3 doses per quart jar.  that assumes 5 grams of dried mycellium per jar.  under top conditions i'll be impressed if you come up with 5 grams.  after that, i'll be double impressed if that mycellium has any more than traces of psilo alkaloids in it.  think about this: how many doses do you get out of 5 dry grams of fruit bodies?  2 semi-strong ones?  3 decent ones?  (or if you're like me, a single dose  :cool:)  right of the bat we're assuming there's exactly as much psilo* in mycellium as there is in mature fruits.  he also makes the assumption that you're retaining the psilosin as well and is figuring that into his math which is a problem because things like... heat, light, and oxygen break it down quite rapidly... how 'bout that 200 degree F method for drying mycellium in your oxygen-containing-earth-atmosphere in your grow room that he reccomends outfitting with flouro grow lights?  see where i'm going with that?  :wink:

quick reality check:  if it were this easy to do, why isn't it done?  if you could turn out "5000 doses a week" why isn't psilocybin a street drug?

anyway, yeah, you can find dextrose at a brewer supply, health food stores, and is even sold as a suppliment.  dextrose is another name for glucose.  you can even find a number of glucose products for diabetics, and i've seen just 1lb bags of it in health food stores before.

calcium carbonate is sometimes found in garden shops or hardware stores.  its also the main ingredient in antacids and calcium suppliments, perhaps you can find pure calcium carbonate tablets meant for human consumption that can be ground to a powder with a mortar & pestle or the like.  eggshells are about 95% calcium carbonate so again once washed you might be able to grind them to a powder and use that in a pinch.  cheap sidewalk chalk is usually straight calcium carbonate also.

potassium phosphate dibasic will be harder to come by.  its easy to obtain from a chemical supplier, and the only place you might find it locally is if you have a specialty hydroponic shop in your neighborhood.

good luck.  i hope you can prove us wrong.  either way you should make a log and take pictures, so even if (when) its a complete failure we can have a nice document to point to and say "there, he did it by the instructions and it didn't work!" :-)

Edited by creamcorn (04/17/06 08:00 PM)

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OfflineAlKaBonGG
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Registered: 04/09/06
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Re: A li'l help with aquiring ingrediants for the medium required for Gottliebs extract tek please? [Re: creamcorn]
    #5526806 - 04/17/06 08:26 PM (18 years, 1 month ago)

Thanks again Creamcorn :smile2:!  REALY good lookin out!  See thats just the kinda info Im lookin to know.  I realy apreciate you takin your time to explane it to me like that. Im gonna tryan get alla my suplies by the weekend but I stil might be short the presure cooker but I'l prolly be able to get started in a coupla weeks or so. I'l post up whatever results I come up with!  Waddafukk, if it dont work out to good I'l study up here and grow out some shrooms :yesnod:!  Either way, Imma like it here!  Blessins on Ya Creamcorn!

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Invisiblecreamcorn
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Re: A li'l help with aquiring ingrediants for the medium required for Gottliebs extract tek please? [Re: AlKaBonGG]
    #5526980 - 04/17/06 09:03 PM (18 years, 1 month ago)

well i'll save you a little more trouble, a pressure cooker isn't totally necessary. many folks use liquid cultures as a starter for their mushroom grows, and sterilize them in a microwave or by steaming them (take a big pot, put a rag on the bottom so the jar isn't touching the pot, fill pot to about 1/3 the height of your jar, and gently boil for about an hour, let cool completely for many hours while still in pot)... look around the cultivation board for "LC" posts and you'll find more info about it than you know what to do with. you're basically making a liquid culture here that's supposedly fortefied with the nutrients required for alkaloid production. your purpose is different (you want to extract from mycellium rather than use the mycellium to start a grow) but the method to sterilize it is the same and doesn't require a pressure cooker.

of course if you plan on eventually growing all the way a PC is a really useful tool and won't go to waste. if you plan on growing you can also use a similar method as this whole mess without most of the steps - simply take fully grown mushrooms, dry them, grind into a powder in a coffee grinder, and soak that powder in methanol (other solvents like grain alcohol, or isopropyl alcohol will work too), strain, and boil off excess liquid until you've got crystals. the difference with the latter is you're using actual mushrooms that actually contain psilocybin, so it actually works. :-)

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Offlineblackout
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Re: A li'l help with aquiring ingrediants for the medium required for Gottliebs extract tek please? [Re: creamcorn]
    #5528307 - 04/18/06 04:44 AM (18 years, 1 month ago)

After reading many, many posts on the subject I concluded that his figures were out by a factor of 10

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OfflineAlKaBonGG
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Registered: 04/09/06
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Last seen: 18 years, 11 days
Re: A li'l help with aquiring ingrediants for the medium required for Gottliebs extract tek please? [Re: blackout]
    #5528385 - 04/18/06 06:33 AM (18 years, 1 month ago)

Hey Blackout :smile2:! Hey Creamcorn :smile2:!  Thanks for takin the time to help a nube!  Cool tip on the microwave Creamcorn.  and WOW!  What a heavy factor to be off by Blackout. Thanks for that insight.  So then by your experiances the entire flaw with Gottliebs tek is that theres not enoughf Psilocybin in the mycelium to extract. Thanks for helpin me understand that.  Im startin to lean twards growin out some shrooms and mabey then doin an extract :naughty:.  Dose that sound a bit more feasable?  Yous guys are a big help allready. Thanks!

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Offlineblackout
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Re: A li'l help with aquiring ingrediants for the medium required for Gottliebs extract tek please? [Re: AlKaBonGG]
    #5528397 - 04/18/06 06:43 AM (18 years, 1 month ago)

I did a lot of reading, and some small experiments. His figures sounded way to much. I doubt he ever did them himself and lost a decimal place somewhere. Agar did do experiments in a large 5 gallon vat, it had a poor yeild, did seem 1/10th. It was also not very potent.

I think it still has some potential (with a huge sterile steup) but you are better off starting with cubensis or easy to grow sclerotia. If you are after something easy to grow and foolproof then sclerotia is the way to go. I see it as the first step before actual shrooms.

Studies also show that active levels change a lot, so potency from day to day, batch to batch changes a lot. Contams is a major problem too. It is hard to tell if a LC is contaminated, you could easily poison yourself.

http://www.shroomery.org/forums/showflat.php?Cat=0&Board=Forum2&Number=4243815

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OfflineAlKaBonGG
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Registered: 04/09/06
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Last seen: 18 years, 11 days
Re: A li'l help with aquiring ingrediants for the medium required for Gottliebs extract tek please? [Re: blackout]
    #5532585 - 04/19/06 07:08 AM (18 years, 1 month ago)

Thanks alot Folks!  Im takin alla your advice to heart and Im gonna work on growin out some Shrooms.  Maby sometimes later after I got the Shrooms goin Il try Gottliebs tek for kiks.  Thanks for settin me straight and Il see ya's on the forrums :smile: !

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