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Offlinerastavibes727
Dance cause weare free
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Registered: 04/04/06
Posts: 22
Loc: Florida
Last seen: 17 years, 6 months
I think I made a mistake...
    #5520401 - 04/16/06 08:30 AM (17 years, 9 months ago)

Last night, me and my friend who are doing our first grow together gre impatient and inoculated the jars after only cooling them for about 3 hours (using the PF Tek). I know it was foolish to do this, but we werer just way too excited to get this started. But anyhow, my question is, should I wait for and pray for colonization, or should I reinoculate while the substrate is sitll usable, because I've got some more spores laying around.

Any help would help, I tried searching but found next to nothing.


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The above post is 100% fictional and intended for entertainment purposes only.


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Offlinekanpan
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Re: I think I made a mistake... [Re: rastavibes727]
    #5520405 - 04/16/06 08:32 AM (17 years, 9 months ago)

Did they feel warm/Hot?


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Offlinerastavibes727
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Registered: 04/04/06
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Loc: Florida
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Re: I think I made a mistake... [Re: kanpan]
    #5520408 - 04/16/06 08:35 AM (17 years, 9 months ago)

Yeah, they were slightly warm for the most part.


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The above post is 100% fictional and intended for entertainment purposes only.


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InvisibleHolydiver
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Re: I think I made a mistake... [Re: rastavibes727]
    #5520426 - 04/16/06 08:43 AM (17 years, 9 months ago)

Maybe you got lucky and the outer edges near the glass was cool enough, but doubtful. All that time to prepare substrate, why not wait an extra 3 hours?


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Offlinerastavibes727
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Registered: 04/04/06
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Loc: Florida
Last seen: 17 years, 6 months
Re: I think I made a mistake... [Re: Holydiver]
    #5520431 - 04/16/06 08:46 AM (17 years, 9 months ago)

It was getting pretty late and I had prior engagements the next morning, but these are now cancelled so I can re-inoculate. I admit I was an idiot, bu I'm guessing reinoculation is the only option.


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The above post is 100% fictional and intended for entertainment purposes only.


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Invisiblebartak59
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Registered: 04/03/06
Posts: 78
Loc: Bomba Shack
Re: I think I made a mistake... [Re: Holydiver]
    #5520433 - 04/16/06 08:46 AM (17 years, 9 months ago)

If the jar is warm to the touch, then the inside of the cake is even going to be hotter. I'm not sure what temp kills spores though.


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Offlinemaxpower210
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Registered: 03/24/06
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Re: I think I made a mistake... [Re: bartak59]
    #5520518 - 04/16/06 09:33 AM (17 years, 9 months ago)

this has happened to me, i waited like 4-5 hours on my first grow and it was getting late. 50% of my jars colonized, but some of those only on one site. it sucked. they were only slightly warm on the outside. now i just go to bed and inoculate in the morning before work. it only takes 5 mins and worry free. i would just reinoculate. good luck though.


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Offlineunseenat17
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Registered: 11/28/05
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Re: I think I made a mistake... [Re: bartak59]
    #5520551 - 04/16/06 09:52 AM (17 years, 9 months ago)

Quote:

bartak59 said:
I'm not sure what temp kills spores though.



meh...ive heard the lil bastards are tough....once i did the same with lc, after i checked em out in 5 days...nuttin...so i planned on re-innoculating...low and behold two weeks later after having my incubator not plugged in...i look inside and there were lil white fuzzy spots all over the jar...woot! im jus mad the incubator wasnt on the whole time to speed up the process. it seems thermal death for cube myc is 106...and it seems for spores...i found this quote on the web "It has been reported that the thermal death point of spores in soil is 30 minutes at 122 oF (50 C)," Wuest and Moore, 1972.
Hard to say what the temp was in those jars...so who knows. I would innoc some different jars up, and jus let those ones sit and wait. GL.


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Offlinerastavibes727
Dance cause weare free
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Registered: 04/04/06
Posts: 22
Loc: Florida
Last seen: 17 years, 6 months
Re: I think I made a mistake... [Re: unseenat17]
    #5522126 - 04/16/06 06:41 PM (17 years, 9 months ago)

Yeah, I reinoculated. My clumsy ass ended up pricking myself with the syringe and hit an artery in my finger, so I was spewing blood. I managed to clean and re-sanitize everything, though, so I'm hoping everytihng will turn out okay.

Thanks a ton for the help guys.


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The above post is 100% fictional and intended for entertainment purposes only.


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OfflineCubenisseur
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Registered: 12/04/05
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Loc: Indian Land
Last seen: 13 years, 10 months
Re: I think I made a mistake... [Re: rastavibes727]
    #5522165 - 04/16/06 06:49 PM (17 years, 9 months ago)

Smoking and innoculating don't go together well.


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Offlinebongtoke
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Registered: 11/14/05
Posts: 1,393
Last seen: 8 years, 10 months
Re: I think I made a mistake... [Re: Cubenisseur]
    #5522269 - 04/16/06 07:25 PM (17 years, 9 months ago)

it doe snt matter when you inoculate as long as the jars feel cool..i dopnt wait until morning..i wait 3-5 hours and then there usually cool to the touch and inside...i never wait 24 hours to inoculate..so your mistake isnt there..as long as the jars were cooled yur fine in that area


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Invisiblebartak59
KOWALSKI

Registered: 04/03/06
Posts: 78
Loc: Bomba Shack
Re: I think I made a mistake... [Re: bongtoke]
    #5522515 - 04/16/06 08:38 PM (17 years, 9 months ago)

damn, how did you manage to prick yourself with that big needle??


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