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Offlinekranked
it'smushtastic!!

Registered: 08/31/04
Posts: 106
Last seen: 16 years, 5 months
Liquid Cultures
    #5518610 - 04/15/06 10:59 AM (17 years, 9 months ago)

I was having problems with getting my jars to colonize (PF-tek). I made a post a couple of months ago and everyone here helped me work out possible variables that I may have over looked. It appeared as though I was doing everything right. My jars weren't showing any growth not even contamination (which was suprising after even leaving the lids off and blowing misc. crap into them just to see if I could get mold to grow.) after a fairly substantial period of time. Summers coming up and I'll have a little more free time on my hands so I figured I would attempt another grow.

I've ordered a spore syringe from spores101 (P. Cubensis b+). I'll be using the PF-tek again. This time to ensure that there is actual mycellium in my jars I was thinking of using a liquid culture. If anyone has used this method I would like to here any comments or tips they might have. By doing this I hope to confirm the problem is the substrate and not the syringes.

Thanks alot.


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"When it went off in New Mexico, that first atomic bomb, we thought of Alfred Nobel and his hope, his vain hope, that dynamite would put an end to all war"

-Robert Oppenheimer 1945


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Offlineshroomballa
ShroomBalla
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Registered: 03/26/06
Posts: 263
Loc: Montana
Last seen: 16 years, 6 months
Re: Liquid Cultures [Re: kranked]
    #5518708 - 04/15/06 11:28 AM (17 years, 9 months ago)

I used the simple LC method (honey water, 1 hole in lid, boiled not PCed) a couple weeks ago and was happy with the results. Just yesterday i shot up a polyfill lid LC jar (PCed) I'll post results of that vs the other method when I start to see growth. But already, it's much more sterile than the simple method


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Anything I say is fictional. Duh.


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Offlineskeletor
the dude
Registered: 09/06/05
Posts: 1,235
Loc: heaven on earth
Last seen: 17 years, 4 months
Re: Liquid Cultures [Re: shroomballa]
    #5519509 - 04/15/06 09:57 PM (17 years, 9 months ago)

get some spore tape over the hole.
swirl the water 1 2 or 3 times a day but try not to get the sporetap wet because contams could then get through your tape.
use a marble or a rock, if you pick rock find something pretty ha.
even think about putting in an injection port. ie some silicon or caulking. then you can inject in open air and not have to worry about contams.


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im sorry about your mother. She was a terrible attrative woman.

Get back to nature; hunting burgers and gathering fries.


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