Home | Community | Message Board


Azarius
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop: ½ Pint Jars, Brown Rice Flour, Pressure Cooker, Wild Bird Seed

Jump to first unread post. Pages: 1
Offlinetullycraft
newbie
Registered: 01/16/02
Posts: 48
Last seen: 16 years, 1 month
Those puertos-- birdseed and partial sterilization
    #551626 - 02/14/02 07:15 PM (16 years, 4 months ago)

Came home to a happy sight today.

I made up some jars with Ryche's birdseed recipe last week, only to discover that my roommate had completely fucked my pressure cooker (tried to cooked some beans with it and let the water boil away, thereby riasing the temperature too high which then melted the alloy pellet in the safety valve...). I had read in one of Anno's posts that it is possible to sterilize grain via multiple boilings... so I figured what the hell. While I was waiting for my new pressure cooker to be delivered... I decided to try out the partial sterilization method.

I boiled the jars for one hour the first day, waited 24 hours, boiled again, waited 48 hours and put them in the oven at 250 degrees, and then to be safe, boiled them again a day later. I opened one jar to see if the substrate was still moist enough.. and it was. I tossed its contents and got to work on the other jars.

So, I went ahead made up 7 syringes with my puerto print and used two of them to innoculate the jars and placed them in my nifty incubation chamber on Saturday night. I saw nothing at all except the tiniest pin-sized signs of growth last night. I came home today, and the jars went buck wild... one of them is 1/3 covered with mycellium.

These babies grow fast... also, no signs of molds or greasy bacteria contams yet, and I smell nothing through the filter disks that indicates anything undesirable cohabitants in the jars.

I will post further details as they develop. If these jars do not fall victim to contams, it would be good evidence that partial sterilization can work with birdseed subtrates, which might bode well for those who cannot afford a PC but want to try non BRF subtrates.

-tc


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineAnnoA
Experimenter
 User Gallery

Folding@home Statistics
Registered: 06/18/99
Posts: 24,123
Loc: my room
Last seen: 10 days, 13 hours
Re: Those puertos-- birdseed and partial sterilization [Re: tullycraft]
    #551638 - 02/14/02 07:32 PM (16 years, 4 months ago)

Good to hear of your apparent success.

Fractional sterilisation works, I tried it myself not so long ago.

I followed this standard procedure, except that I boiled the jars for 45 minutes at the lat boil:

Tyndallization (fractional sterilisation) process

1) Steam heating to 90-100 ?C for 30 min
Vegetative cells are destroyed but endospores survive

2) Incubate at 37 ?C overnight
Most spores germinate

3) Second heat treatment 90-100 ?C, 30 min
Germinated spores are killed

4) Second incubation at 37 ?C overnight
Remaining spores germinate

5) Third heat treatment 90-100 ?C, 30 min
Germinated spores are killed


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Amazon Shop: ½ Pint Jars, Brown Rice Flour, Pressure Cooker, Wild Bird Seed

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Similar ThreadsPosterViewsRepliesLast post
* Oven sterilization? jonas 952 13 11/08/04 03:27 AM
by dmc_
* Sterilized Jars Upkeep and freshness Boppity604 823 2 05/27/02 04:29 PM
by Boppity604
* sterilization zulu 1,306 19 11/12/03 12:00 PM
by Anno
* Need advice on sterility & trading spores. The_Gnome_King 1,758 14 06/05/01 12:16 AM
by Azure
* Every how often do i have to sterilize the misting water? Sterile 652 3 05/26/04 11:48 AM
by Sterile
* what do you think about this subtrate pic? manvstrees 817 6 12/09/03 01:11 AM
by ask
* month old subtrate..... Shroomel 866 3 09/18/01 08:33 AM
by BeppoMarx
* sterile shrooms strang 705 4 09/18/01 03:05 PM
by strang

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Roadkill, Magash, Shroomism, george castanza, RogerRabbit, Citric, total, FooMan, 13shrooms, stonesun, cronicr, PussyFart, Tmethyl, Pastywhyte, bodhisatta
526 topic views. 21 members, 63 guests and 16 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:

Please support our sponsors.

Copyright 1997-2018 Mind Media. Some rights reserved.

Generated in 0.027 seconds spending 0.006 seconds on 19 queries.