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olpatlo
Stranger
Registered: 06/14/04
Posts: 14
Last seen: 17 years, 9 months
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print... syringe... contam ?
#5512278 - 04/13/06 01:05 PM (17 years, 9 months ago) |
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alright so i got me a print, i boiled distilled water, made sure my syringe was sterile, used a cleaned non teflon pot, i even decided to rinse out the syringe once for safe measures. scraped off the spores from print into baggie and squirt the cooled distilled water into the baggie, sealed bag mixed it up, sucked it back up and put away for storage, into 2 zip[lock bag and in the fridge.
ok what my question is. is that, is there any way of contaminations to get in with the syringe making it, ultimatly a bad syringe so all the cakes i do, they will be contaminated from some error in the proccess of print to syringe to cake.
also if you dont mind, check out another post of mine, and tell me your thoughts on it, id really appreciate it. thank you every1 for reading. i appreciate posts 
http://www.shroomery.org/forums/showflat.php/Cat/0/Number/5489857/an/0/page/0
Edited by olpatlo (04/13/06 01:06 PM)
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mungojerry
free as a bird

Registered: 02/25/06
Posts: 3,598
Loc: Nappy Dub
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Re: print... syringe... contam ? [Re: olpatlo]
#5512575 - 04/13/06 02:49 PM (17 years, 9 months ago) |
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this happened to me a week ago i made the syringes then put then directly in the fridge and it was contamed i dunno maybe we need to wait 24 hours before we put em in the fridge
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skeletor
the dude
Registered: 09/06/05
Posts: 1,235
Loc: heaven on earth
Last seen: 17 years, 4 months
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Re: print... syringe... contam ? [Re: mungojerry]
#5512964 - 04/13/06 04:53 PM (17 years, 9 months ago) |
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did you do this is a 'cleanroom' glove box or under a flow hood?
-------------------- im sorry about your mother. She was a terrible attrative woman. Get back to nature; hunting burgers and gathering fries.
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cappa
Nerd
Registered: 02/12/06
Posts: 854
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Re: print... syringe... contam ? [Re: skeletor]
#5513049 - 04/13/06 05:23 PM (17 years, 9 months ago) |
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1. Make a still air glovebox.
2. Learn about LCs.
3. Scape a little spores into your LC inside of your glovebox.
Damn I love this hobby!
-------------------- Their are 10 types of people. Those that understand binary, and those who don't. ~Cappa.
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skeletor
the dude
Registered: 09/06/05
Posts: 1,235
Loc: heaven on earth
Last seen: 17 years, 4 months
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Re: print... syringe... contam ? [Re: cappa]
#5513845 - 04/13/06 09:23 PM (17 years, 9 months ago) |
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make a syringe out of a mushy whos veil has yet to break. ive yet to try this i plan to next time.
-------------------- im sorry about your mother. She was a terrible attrative woman. Get back to nature; hunting burgers and gathering fries.
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Zen Peddler


Registered: 06/18/01
Posts: 6,379
Loc: orbit
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Re: print... syringe... contam ? [Re: olpatlo]
#5514698 - 04/14/06 02:35 AM (17 years, 9 months ago) |
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most contaminants are airborn - get yourself a glove box and your contamination rate will decrease profoundly.
--------------------
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olpatlo
Stranger
Registered: 06/14/04
Posts: 14
Last seen: 17 years, 9 months
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Re: print... syringe... contam ? [Re: Zen Peddler]
#5525075 - 04/17/06 01:38 PM (17 years, 9 months ago) |
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damn i had a clean glove box and all at the time, i just didnt do enough research on the syringe tech.
cappa, you mentioned above "2. Learn about LCs."
what are these LCs you speak of?
well i got some cakes going, to the most part they look contam free, but theres some secions where it looks like brf is stuck or its a wetspot, when i move them to fruiting is when ill know.
ya so i got another print on the way, im gona do this right this time, if ya can point out and explain this Lcs u speak of id really appreciate it.
thx again
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skeletor
the dude
Registered: 09/06/05
Posts: 1,235
Loc: heaven on earth
Last seen: 17 years, 4 months
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Re: print... syringe... contam ? [Re: olpatlo]
#5525252 - 04/17/06 02:23 PM (17 years, 9 months ago) |
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honey or karo water. you inject a cc or so into this sterilized nutrient water and myc grows. you can turn 10 cc in to 100 with very little effort.
-------------------- im sorry about your mother. She was a terrible attrative woman. Get back to nature; hunting burgers and gathering fries.
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