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OfflineSolidcell
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Registered: 01/20/06
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Damn, 3 out of the 4 Z jars I have contamed. Can you tell which step caused it? *pics*
    #5499532 - 04/10/06 11:38 AM (17 years, 9 months ago)

These pics were taken after an 18hr dunk in 1:10 H2O2/H20 in the fridge.

This is the worst jar of them all.


Last night however, the worst jar had looked like the other jars do now.





Some pictures are of the same jar and I may have not gotten a pic of them all (I'm talking about the 3 that contamed, the 4th is free of green).

The first sign of any green was in the worst jar about a day or two ago when I noticed that there was a shade of green showing through from behind the myc. I'm hoping the 4th doesn't have it as well and that it just hasn't showed any signs yet.

So is it possible to tell whether the contam was from the inoculation or from the jar? I have 2 other strains going that aren't done yet that have 4 jars and 3 jars (I have 11 jars in total) that have no signs at all of contam. So I'm guessing that it was from the Z syringe and not from the jars, but then why would there be one jar that's fine (hopefully)?


--------------------
Psychedelic Jar Project

"It is dangerous to be right when the government is wrong." -Voltaire

"Everything that I accepted as being true up to now I acquired from the senses or through the senses. However, I have occasionally found that they deceive me, and it is prudent never to trust those who have deceived us, even if only once." -Descartes


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Offlineskeletor
the dude
Registered: 09/06/05
Posts: 1,235
Loc: heaven on earth
Last seen: 17 years, 4 months
Re: Damn, 3 out of the 4 Z jars I have contamed. Can you tell which step caused it? *pics* [Re: Solidcell]
    #5499638 - 04/10/06 12:11 PM (17 years, 9 months ago)

no really. though if it was contaminated during inoculation it would normally expect to see green well before 100%. maybe the inside of them weren't fully cononised and the dunking transfered some mold spores inside.


--------------------
im sorry about your mother. She was a terrible attrative woman.

Get back to nature; hunting burgers and gathering fries.


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OfflineSolidcell
tolerance++;
Male

Registered: 01/20/06
Posts: 754
Loc: Memphis & 53rd
Last seen: 15 years, 4 months
Re: Damn, 3 out of the 4 Z jars I have contamed. Can you tell which step caused it? *pics* [Re: skeletor]
    #5499731 - 04/10/06 12:29 PM (17 years, 9 months ago)

I saw the green coming through the surface in the one that showed signs yesturday. I don't think mold could contam a cake while being dunked, I think it would have had to have been there before. I'm thinking it was the syringe that was contaminated since it's only this strain that has shown contam signs, but it's weird how it took till the end of colonization for it to appear on the surface.


--------------------
Psychedelic Jar Project

"It is dangerous to be right when the government is wrong." -Voltaire

"Everything that I accepted as being true up to now I acquired from the senses or through the senses. However, I have occasionally found that they deceive me, and it is prudent never to trust those who have deceived us, even if only once." -Descartes


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InvisibleSimisu
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Registered: 08/08/03
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Re: Damn, 3 out of the 4 Z jars I have contamed. Can you tell which step caused it? *pics* [Re: Solidcell]
    #5499753 - 04/10/06 12:33 PM (17 years, 9 months ago)

that ain't contaminated... that's just brusing!
it's fine...


--------------------
:mushdance::sanpedro::peyote::mushroom2: :heart: Shr:supershroom::supershroom:mery :heart: :mushroom2::peyote::sanpedro::mushdance:
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OfflineSnaggletooth
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Re: Damn, 3 out of the 4 Z jars I have contamed. Can you tell which step caused it? *pics* [Re: Simisu]
    #5499761 - 04/10/06 12:36 PM (17 years, 9 months ago)

^^^could be....here is one that is contaminated




--------------------


Atheist Chat


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Offlinescatmanrav
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Registered: 05/08/04
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Re: Damn, 3 out of the 4 Z jars I have contamed. Can you tell which step caused it? *pics* [Re: Snaggletooth]
    #5499764 - 04/10/06 12:38 PM (17 years, 9 months ago)

100% sure that is bruising and not contamination. Adding h2o2 to the water will do that.


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


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OfflineSolidcell
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Registered: 01/20/06
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Re: Damn, 3 out of the 4 Z jars I have contamed. Can you tell which step caused it? *pics* [Re: Solidcell]
    #5499767 - 04/10/06 12:39 PM (17 years, 9 months ago)

It's pretty green, and I couldn;t see how it's bruising if I haven't touched or moved the cakes, only scraped verm and added 1:10 solution. The green is spreading, so unless I'm crazy, it's mold.


--------------------
Psychedelic Jar Project

"It is dangerous to be right when the government is wrong." -Voltaire

"Everything that I accepted as being true up to now I acquired from the senses or through the senses. However, I have occasionally found that they deceive me, and it is prudent never to trust those who have deceived us, even if only once." -Descartes


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OfflineSnaggletooth
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Re: Damn, 3 out of the 4 Z jars I have contamed. Can you tell which step caused it? *pics* [Re: Solidcell]
    #5499772 - 04/10/06 12:41 PM (17 years, 9 months ago)

I had a case that self bruised, not by touching it, I thought It was a contam.

Just wait it out.......


--------------------


Atheist Chat


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OfflineSolidcell
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Male

Registered: 01/20/06
Posts: 754
Loc: Memphis & 53rd
Last seen: 15 years, 4 months
Re: Damn, 3 out of the 4 Z jars I have contamed. Can you tell which step caused it? *pics* [Re: Solidcell]
    #5499774 - 04/10/06 12:41 PM (17 years, 9 months ago)

I guess I'll just watch and see if it keeps spreading. The only way I forsee telling if its bruising of contam, would a contam stop spreading in a soultion like that where bruising would keep happening, and would it not radiate from a spot like it is now and instead bruise everywhere? I don't want to put the 3 bruised/contamed cakes in with the 1 good one (hopefully good) and possibly contam that one. I'm going to be rolling as well.


--------------------
Psychedelic Jar Project

"It is dangerous to be right when the government is wrong." -Voltaire

"Everything that I accepted as being true up to now I acquired from the senses or through the senses. However, I have occasionally found that they deceive me, and it is prudent never to trust those who have deceived us, even if only once." -Descartes


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InvisibleSimisu
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Registered: 08/08/03
Posts: 5,435
Loc: Israeli in Flag
Re: Damn, 3 out of the 4 Z jars I have contamed. Can you tell which step caused it? *pics* [Re: Snaggletooth]
    #5499790 - 04/10/06 12:46 PM (17 years, 9 months ago)

although i have no expiriance with H2o2 i'd go with scatmanrav and say that that's the cause... it's not contemination for sure though!


--------------------
:mushdance::sanpedro::peyote::mushroom2: :heart: Shr:supershroom::supershroom:mery :heart: :mushroom2::peyote::sanpedro::mushdance:
      Visit & Support Free Spore Ring Earth
      :sun: Please help spread live Salvia Divinorum :sun:



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OfflineSolidcell
tolerance++;
Male

Registered: 01/20/06
Posts: 754
Loc: Memphis & 53rd
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Re: Damn, 3 out of the 4 Z jars I have contamed. Can you tell which step caused it? *pics* [Re: Solidcell]
    #5499803 - 04/10/06 12:49 PM (17 years, 9 months ago)

Oh man, that would be a relief if it's only bruising. Well, I'll roll them all, the pure white one first just in case. If it's a contam and it spreads, you guys are in big trouble I tell you!

Thanks for the input guys.


--------------------
Psychedelic Jar Project

"It is dangerous to be right when the government is wrong." -Voltaire

"Everything that I accepted as being true up to now I acquired from the senses or through the senses. However, I have occasionally found that they deceive me, and it is prudent never to trust those who have deceived us, even if only once." -Descartes


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InvisibleRoadkillM
Retired Shroomery Mod
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Registered: 12/11/01
Posts: 22,674
Loc: Montana
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Re: Damn, 3 out of the 4 Z jars I have contamed. Can you tell which step caused it? *pics* [Re: Solidcell]
    #5499827 - 04/10/06 12:58 PM (17 years, 9 months ago)

this one looks like bruising....




and this one looks like its contaminated with the mean GREEN...




tc


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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Offlineskeletor
the dude
Registered: 09/06/05
Posts: 1,235
Loc: heaven on earth
Last seen: 17 years, 4 months
Re: Damn, 3 out of the 4 Z jars I have contamed. Can you tell which step caused it? *pics* [Re: Roadkill]
    #5499835 - 04/10/06 01:02 PM (17 years, 9 months ago)

if it looks powdery its mold youll see what i mean when u dump out a contamed cakes. green icing sugar!


--------------------
im sorry about your mother. She was a terrible attrative woman.

Get back to nature; hunting burgers and gathering fries.


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OfflineSolidcell
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Male

Registered: 01/20/06
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Loc: Memphis & 53rd
Last seen: 15 years, 4 months
Re: Damn, 3 out of the 4 Z jars I have contamed. Can you tell which step caused it? *pics* [Re: skeletor]
    #5499880 - 04/10/06 01:24 PM (17 years, 9 months ago)

Aight so I rolled them an they're now in the FC. I'm going to start the light cycle tomorrow. It will be from 3am-3pm (earlier if it doesn't wake me and later if the sun covers the light eminating from under the bed).

You guys were right about the bruising because when I took out the cakes, they were tight against the glass (I guess the soak helped press them up against it) which would bruise them as well. To get them out I had to pull a little from the base and it brusied the same color after looking where I was holding.

The surface had only colonized a day before dunking them, would this be ample for letting the inside fo the cake colonize?






The rH ranges from 95-96, and the temp goes from 67.5-71.


--------------------
Psychedelic Jar Project

"It is dangerous to be right when the government is wrong." -Voltaire

"Everything that I accepted as being true up to now I acquired from the senses or through the senses. However, I have occasionally found that they deceive me, and it is prudent never to trust those who have deceived us, even if only once." -Descartes


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InvisibleRoadkillM
Retired Shroomery Mod
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Registered: 12/11/01
Posts: 22,674
Loc: Montana
Trusted Cultivator
Re: Damn, 3 out of the 4 Z jars I have contamed. Can you tell which step caused it? *pics* [Re: Solidcell]
    #5499894 - 04/10/06 01:29 PM (17 years, 9 months ago)

Quote:

The rH ranges from 95-96, and the temp goes from 67.5-71.




Increase your humidity...

and get your temps up to around 76 degrees F.



tc


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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OfflineSolidcell
tolerance++;
Male

Registered: 01/20/06
Posts: 754
Loc: Memphis & 53rd
Last seen: 15 years, 4 months
Re: Damn, 3 out of the 4 Z jars I have contamed. Can you tell which step caused it? *pics* [Re: Roadkill]
    #5499923 - 04/10/06 01:41 PM (17 years, 9 months ago)

The pump I have is on max, and when it was off completely, the rH was still reading 95-96 in a plain perlite container. It doesn't seem like it should have been reading only 95 or 96 in a perlite tub with no FAE. So I'm not sure where the pump should be set to have good FAE and rH. Any suggestions on what I should do about the temps?

The cakes are rolled in verm, so should that help with the rH as well. I can't imagine it doing too much though.


--------------------
Psychedelic Jar Project

"It is dangerous to be right when the government is wrong." -Voltaire

"Everything that I accepted as being true up to now I acquired from the senses or through the senses. However, I have occasionally found that they deceive me, and it is prudent never to trust those who have deceived us, even if only once." -Descartes


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OfflineSolidcell
tolerance++;
Male

Registered: 01/20/06
Posts: 754
Loc: Memphis & 53rd
Last seen: 15 years, 4 months
Re: Damn, 3 out of the 4 Z jars I have contamed. Can you tell which step caused it? *pics* [Re: Solidcell]
    #5499939 - 04/10/06 01:48 PM (17 years, 9 months ago)

If it comes down to it, I have a tub I'm making for casings and I have to calibrate the rH. It has a CM hosed to it running 24/7, so it gets 100% rH (I'm trying to get it to 85 for the casings). I won't need it for a while, so I could use it for cakes in the mean time.

Come to think of it, it read 96 in that tub as well when it was clearly 99 or 100. So I'm not sure how I can know when the PMP is higher than 96...

It's supposed to read up to 99, but it has never shouwn an rH of more than 97 (it was only briefly, it's max is consistently 96).


--------------------
Psychedelic Jar Project

"It is dangerous to be right when the government is wrong." -Voltaire

"Everything that I accepted as being true up to now I acquired from the senses or through the senses. However, I have occasionally found that they deceive me, and it is prudent never to trust those who have deceived us, even if only once." -Descartes


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