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Shop: North Spore Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies   Mushroom-Hut Liquid Cultures

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OfflineBMArts
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Milky LC = contam?
    #5495527 - 04/09/06 03:11 AM (17 years, 10 months ago)

From another post that isn'T being answered anymore:

Quote:

ShrOOmSynEgry said:
Quote:

vintage_gonzo said:
a milky dust? isnt that the exact description of a healthy LC?



milky dust = contams :thumbdown:, clearish white boogers = mycelium :thumbup:






Could you please specify or could someone else confirm this?
How do you know very fine dust is a signe of contam? Which contam would this be? I just knocked up a LC that is looking quite milky and dusty. Does this mean its contamed? It doesn't seem that anything's wrong with it. (no smell etc.) So far its to early to see myc anyway... but now that you mention this I wonder if I should be worried


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Invisiblecreamcorn
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Re: Milky LC = contam? [Re: BMArts]
    #5495586 - 04/09/06 04:47 AM (17 years, 10 months ago)

if it turns milky or dusty, its a contam... if it appeared that way from the start, don't worry about it. you say its too early to see myc, so its also gonna probably be too early to see a contam growth. sometimes some of your LC ingredient (especially true of honey) doesn't dissolve fully and there's sediments... protiens and wax in the case of honey. if it was in there since the start and doesnt seem to be "growing" you're good. when they say milky its more of a change to the color of the liquid itself, of course if you have white mycellium growing its going to get a whitish hue, especially if you shake it up to break up the myc.

you'll also get a good idea when its time to draw a syringe, it will smell horrible if its been contaminated with bacteria.


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OfflineBMArts
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Re: Milky LC = contam? [Re: creamcorn]
    #5495616 - 04/09/06 05:20 AM (17 years, 10 months ago)

Well it did sort of turn that was...
I'm doing malt/dex LC and I filtered the solution with a coffe filter. After boiling it took about half a day till the solution had turned like that.


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OfflineBlue Helix
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Re: Milky LC = contam? [Re: BMArts]
    #5497066 - 04/09/06 04:03 PM (17 years, 10 months ago)

Depending on what you used to start the LC, and I don't know much about honey, you can expect some sediments to form during the pressure cooking. Some of the sediments could make the water appear cloudy, but it doesn't matter. I looked at many good liquid cultures using a high powered compound microscope and found that mycelium love sediments. It often attaches to it and uses the pieces as a platform for growth. That is why as the culture completes, it _should_ become clear between the mycelium blobs, because the mycelium has sequestered the sediment.


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Offlinemogur
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Re: Milky LC = contam? [Re: Blue Helix]
    #5497119 - 04/09/06 04:30 PM (17 years, 10 months ago)

Quote:

Blue Helix said:
.... I looked at many good liquid cultures using a high powered compound microscope and found that mycelium love sediments. It often attaches to it and uses the pieces as a platform for growth. That is why as the culture completes, it _should_ become clear between the mycelium blobs, because the mycelium has sequestered the sediment.




Thank you for a great piece of info, blue helix. Generally, in my experience, I just assume bacterial contamination when a previously clear LC goes cloudy, since I've read that turbidity can be used as a rough measurement of bacterial contamination in microbiology textbooks. And, not wanting to waste the effort put into sterilizing grains, I usually don't inoculate with cloudy cultures. But, maybe a little more venturesome attitude on my part would be beneficial. Will keep a closer eye in the future, since some of the cloudy cultures that I tossed seemed to have quite vigorous growth of opaque white masses.


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OfflineBlue Helix
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Re: Milky LC = contam? [Re: BMArts]
    #5498064 - 04/09/06 09:11 PM (17 years, 10 months ago)

Ok, there must have been some misunderstanding here, so let's sync up to the same page: if a culture becomes cloudy during maturation, it is almost definitely bacteria at work. Such a culture may make it if the mycelium is vigorous enough--I've seen it happen--but it probably won't. If a culture appears cloudy or has particulate matter in it OUT OF THE PRESSURE COOKER, it's just sediments (carmelized sugars and what not). Seeing particles in the solution right out of the pressure cooker is common when using light malt extract but I have no idea if honey is the same. I don't know what that stuff is exactly, but it is always there and from the malt sugar.


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Offlineblackout
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Re: Milky LC = contam? [Re: Blue Helix]
    #5499195 - 04/10/06 09:09 AM (17 years, 10 months ago)

Proteins come out of solution within a few days, you can stick it in the fridge to speed this up. They are bigger globules than baterial sediment. When you shake up a protein LC it is no really milky since it separates, bacterial growth goes cloudly, like yeast in proper bottle conditioned beer.


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