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OfflineZacharian
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Registered: 04/01/06
Posts: 72
Last seen: 17 years, 9 months
Negative air pressure / vacuum environment glove box?
    #5496767 - 04/09/06 02:33 PM (17 years, 9 months ago)

Is there any professional lab equipment that creates a vacuumed environment?

Wouldn't that be the best sterile environment to be working where there is no air at all?

If you were in above the atmosphere in space, would that be sterile?


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Offlinemogur
regnartS

Registered: 11/15/05
Posts: 322
Loc: Puget Sound
Last seen: 11 years, 2 months
Re: Negative air pressure / vacuum environment glove box? [Re: Zacharian]
    #5496870 - 04/09/06 02:59 PM (17 years, 9 months ago)

Yes, theoretically. The less air pressure, the less dust could ferry contams around to waft into your culture. They would just fall flat onto the work surface. In reality, it just doesn't work, though, because leaks will be disastrous. Imagine a leaky glovebox with positive pressure. The leaks push clean air out of the box and everything is fine, as long as the replacement air is filtered to keep contams outside. Now, imagine a negative pressure box. Any leaks will carry outside contams INTO the box, ruining your whole afternoon.


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OfflineZacharian
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Registered: 04/01/06
Posts: 72
Last seen: 17 years, 9 months
Re: Negative air pressure / vacuum environment glove box? [Re: mogur]
    #5496906 - 04/09/06 03:12 PM (17 years, 9 months ago)

Is there a professional piece of equipment that does not allow leaks?

That would fit on a desk? (Not like a vacuum chamber that you would walk into or something)


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Invisiblemusher_420
Stranger

Registered: 08/01/05
Posts: 2,691
Re: Negative air pressure / vacuum environment glove box? [Re: Zacharian]
    #5496927 - 04/09/06 03:21 PM (17 years, 9 months ago)

I really don't think you could make a glove box work with a vacuum. Even if you could have it seal perfectly. You would need some kind of incredibly strong gloves.

Still air glove box or a flow hood if you want to spend some money.


--------------------
My Humidifier Reservoir Expansion Tek

Must read links for beginners

Btw- You are not what you own!


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Offlinemogur
regnartS

Registered: 11/15/05
Posts: 322
Loc: Puget Sound
Last seen: 11 years, 2 months
Re: Negative air pressure / vacuum environment glove box? [Re: Zacharian]
    #5496934 - 04/09/06 03:23 PM (17 years, 9 months ago)

No, there is no pro vacuum box that I know of that is meant for culture work. It is impracticable because there are many much cheaper solutions than depending on pristine seals for sterility. Think about it, what is easier, building a box with a little positive pressure and an input filter, or building a vacuum box and having to seal it like a spacesuit.


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OfflineZacharian
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Registered: 04/01/06
Posts: 72
Last seen: 17 years, 9 months
Re: Negative air pressure / vacuum environment glove box? [Re: mogur]
    #5496958 - 04/09/06 03:29 PM (17 years, 9 months ago)

Hmm this looks interesting

http://cgi.ebay.com/Experimental-Vacuum-...1QQcmdZViewItem

How do you use that? Does it hook up to something?


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Offlinemogur
regnartS

Registered: 11/15/05
Posts: 322
Loc: Puget Sound
Last seen: 11 years, 2 months
Re: Negative air pressure / vacuum environment glove box? [Re: Zacharian]
    #5496999 - 04/09/06 03:43 PM (17 years, 9 months ago)

Okay, I was just lying because it's a well kept secret in shroom cultivation that vacuum is the easiest and cheapest way to achieve reliable sterile conditions. Now that you know the secret, good licks, and don't suck more than you can smell.


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OfflineZacharian
Stranger
Registered: 04/01/06
Posts: 72
Last seen: 17 years, 9 months
Re: Negative air pressure / vacuum environment glove box? [Re: mogur]
    #5497058 - 04/09/06 04:00 PM (17 years, 9 months ago)

I never asked for the cheapest and easiest, and I believe you.

Now, do you know how to use that or does anyone here know?


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OfflineRogerRabbitM
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Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 11 months, 3 days
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Re: Negative air pressure / vacuum environment glove box? [Re: Zacharian]
    #5498304 - 04/09/06 10:29 PM (17 years, 9 months ago)

How long do you think the moisture would last in your mycelium in a vacuum?
RR


--------------------
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semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison


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OfflineDaCultivater
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Registered: 10/08/05
Posts: 289
Last seen: 15 years, 9 months
Re: Negative air pressure / vacuum environment glove box? [Re: RogerRabbit]
    #5498480 - 04/09/06 11:32 PM (17 years, 9 months ago)

i think that it would suck all the air out of the jar so there would be no air for the mycelium to start growth


--------------------
as they said in some movie(i don't remember which one);

a belief is a dangerous thing, people will kill over it; instead of believing you need to find your facts and then have an opinionated idea that you are willing to change in light of new evidence


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