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OfflineZacharian
Stranger
Registered: 04/01/06
Posts: 72
Last seen: 17 years, 9 months
inoculation - is this a correct/viable method?
    #5492319 - 04/08/06 10:10 AM (17 years, 9 months ago)

Is this ok to do this on a grain jar:
1. Cover top of jar with tyvek and put ring on.

2. Cut small slit with razor and cover slit with a piece of masking tape.

3. Pc for 90 minutes.

4. Allow to jar to cool.

5. Sterilize end of needle.

6. Lift tape and inject. quickly put tape back when done.

Is this ok? Does the tape have to be any special tape or it will withstand pc'ing fine - it doesn't lose any adhesive?


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Offlineskeletor
the dude
Registered: 09/06/05
Posts: 1,235
Loc: heaven on earth
Last seen: 17 years, 4 months
Re: inoculation - is this a correct/viable method? [Re: Zacharian]
    #5494013 - 04/08/06 06:08 PM (17 years, 9 months ago)

uh well you will mostlikely need that metal rind with a hole in it. if you just have tyvek as teh lid your jars will really dry out and stall. also dont' bother cutting a hole through the tyvek just inject throught it then put tape over. then the tape never has to withstand PCing. or you could just use microspore tape to tape holes and no tyvek. or a combination.


--------------------
im sorry about your mother. She was a terrible attrative woman.

Get back to nature; hunting burgers and gathering fries.


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OfflineZacharian
Stranger
Registered: 04/01/06
Posts: 72
Last seen: 17 years, 9 months
Re: inoculation - is this a correct/viable method? [Re: skeletor]
    #5494378 - 04/08/06 07:57 PM (17 years, 9 months ago)

Well in the Reckless Straw tek it says this:
Quote:

cut squares of tyvek envelopes found free at your post office..one piece per jar,under the ring..do not use lid..tyvek and ring only..now go ahead and pressure cook for 1 hr at 15psi




Found here: http://www.shroomtalk.com/mushroom/forum6/668.html

Maybe he is doing ok with it because he is only pc'ing for 1 hr?


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Offlineskeletor
the dude
Registered: 09/06/05
Posts: 1,235
Loc: heaven on earth
Last seen: 17 years, 4 months
Re: inoculation - is this a correct/viable method? [Re: Zacharian]
    #5494468 - 04/08/06 08:24 PM (17 years, 9 months ago)

i dunno but i know i see posts every now and then about jars drying and often its beucase of no lid. think of how much more evaporation you get having the full lid giving gas exhange as opposed to just a square inch.
i just use the spore tape over one hole in the metal lid and then i have another hole with caulking blobbed on as an injection site. if you wipe down your needle and inject through this port with a cotton ball or paper towl soaked with alhohole you can do this out in the dirtyest enviroment and not have to woryy about contams


--------------------
im sorry about your mother. She was a terrible attrative woman.

Get back to nature; hunting burgers and gathering fries.


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