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Tattoo
Thread Killer


Registered: 02/23/06
Posts: 1,574
Last seen: 8 years, 25 days
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exposed syringe
#5490097 - 04/07/06 01:33 PM (17 years, 9 months ago) |
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One of my syringes somehow got loose at the injection point and was exposed for an undetermined amount of time (could of been 5 minutes or a few hours, I don't know)- its airtight at the moment, is the syringe contaminated?
Another factor, I am spawning to myconitro which is highly resistant to contamination- so I'm a little wary which way to go on this
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one nigga run, two nigga run
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EquilibriuM
dream stalker

Registered: 07/17/05
Posts: 2,323
Last seen: 16 years, 7 months
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Re: exposed syringe [Re: Tattoo]
#5490167 - 04/07/06 01:54 PM (17 years, 9 months ago) |
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I have no idea. I doubt anyone will...
but how can I get some myco nitro ?
-------------------- HELP!!!!!!!!!
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creamcorn
mad scientist


Registered: 03/13/06
Posts: 2,962
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Re: exposed syringe [Re: Tattoo]
#5490184 - 04/07/06 02:01 PM (17 years, 9 months ago) |
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.... what? what came loose? what was exposed? huh? what do you mean injection point? the needle came off?
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Tattoo
Thread Killer


Registered: 02/23/06
Posts: 1,574
Last seen: 8 years, 25 days
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Re: exposed syringe [Re: creamcorn]
#5490190 - 04/07/06 02:03 PM (17 years, 9 months ago) |
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yeah, it came pre-capped over a needle, but it came off where it screws into the syringe and was exposed to air
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one nigga run, two nigga run
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creamcorn
mad scientist


Registered: 03/13/06
Posts: 2,962
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Re: exposed syringe [Re: Tattoo]
#5490231 - 04/07/06 02:20 PM (17 years, 9 months ago) |
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Well there's no gauruntee but I'd guess its ok. (There wasn't a garuntee the syringe wasn't already contaminated in the first place either right? ) That's a pretty tiny opening and I'm guessing it was loose/exposed in a clean area you were planning on doing your work anyway? Even so, the odds of something landing in there is pretty small, and I'd use it right away - say a bacterial endospore made its way on there, if you go use it up you'll end up with one contaminated inoculation point where that contam landed.... if you let it sit that contaminating spore could germinate and spread through the rest of the syringe.
Either way my bet is that its probably cool, don't sweat it.
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creamcorn
mad scientist


Registered: 03/13/06
Posts: 2,962
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Re: exposed syringe [Re: creamcorn]
#5490259 - 04/07/06 02:30 PM (17 years, 9 months ago) |
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P.S. Come to think of it... I did that back the first time I was handling a syringe. I thought the cap protecting the needle was to be unscrewed, so i twisted, and the whole needle came off in my hand! There was a few seconds of "oh shit" before I screwed it back on but I had no problems.
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vegitative
Stranger
Registered: 01/21/06
Posts: 97
Last seen: 17 years, 7 months
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Re: exposed syringe [Re: creamcorn]
#5490594 - 04/07/06 04:45 PM (17 years, 9 months ago) |
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Quote:
creamcorn said: P.S. Come to think of it... I did that back the first time I was handling a syringe. I thought the cap protecting the needle was to be unscrewed, so i twisted, and the whole needle came off in my hand! There was a few seconds of "oh shit" before I screwed it back on but I had no problems.
I've done that one twice now... as far as i can tell everythings fine (only 3 days into this grow).
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FungusMan
I81U812



Registered: 08/06/05
Posts: 3,112
Loc: Everywhere
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Re: exposed syringe [Re: vegitative]
#5490672 - 04/07/06 05:29 PM (17 years, 9 months ago) |
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Lemme ask you a question of about the same caliber. "I was banging this girl, and I pulled out DURING ejac, rather than the standard "before". Is she pregnant?" There's no way to know until you use it. Good luck!
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skeletor
the dude
Registered: 09/06/05
Posts: 1,235
Loc: heaven on earth
Last seen: 17 years, 4 months
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Re: exposed syringe [Re: FungusMan]
#5490737 - 04/07/06 06:03 PM (17 years, 9 months ago) |
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well i belive the pull out method is about 70% effective. 70% effective with my life just won't cut it though. condoms are 99. something but tehy downgraded that to 97 or something ebucase 3% of people are too stupid to use one correctly.ha
-------------------- im sorry about your mother. She was a terrible attrative woman. Get back to nature; hunting burgers and gathering fries.
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FooMan



Registered: 02/02/05
Posts: 8,957
Loc: Earth
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Re: exposed syringe [Re: Tattoo]
#5490751 - 04/07/06 06:16 PM (17 years, 9 months ago) |
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Quote:
Tattoo said: Another factor, I am spawning to myconitro which is highly resistant to contamination- so I'm a little wary which way to go on this
Spawning or inoculating? If you're spawning, you should already know if the syringe is good or not. If you are injecting your spores directly into myconitro or any other kind of compost/manure, I doubt the spores will germinate at all without some grains mixed in with it. Any substrate can/will contam if you introduce a contaminant to it, even myconitro.
I am just assuming you are talking about spores and not an LC, of course.
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Quick WBS Prep
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Tattoo
Thread Killer


Registered: 02/23/06
Posts: 1,574
Last seen: 8 years, 25 days
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Re: exposed syringe [Re: FooMan]
#5490798 - 04/07/06 06:44 PM (17 years, 9 months ago) |
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crap
Yes you assume correctly- shit... any suggestions? Its that big PE btw foo
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one nigga run, two nigga run
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