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SDP
ChronicAficionado



Registered: 01/21/05
Posts: 1,297
Last seen: 5 months, 28 days
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Myecelium dead. :( Can i re-pressure cook and re-inoculate?
#5486030 - 04/06/06 10:29 AM (17 years, 9 months ago) |
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Hello. I was trying to make a new incubator (adjusting settings) and i think i fried my mycelium... i donno though, some have growth most dont (been a week and a half) and the growth on the ones that have it (little tiny strips) hasnt grown in about 5-6 days... and its greyish white now, not rhizomorphic at all.
Yes i waited for the jars to cool off before initial inoculation. 
So... can i re-pressure cook the jars, re-inoculate, and make use of about 18 uncolonized or barely colonized jars? Just curious about input.. Thanks!
-SDP
-------------------- Teonanacatl, open up my eyes This sacrament, this prayer, beyond the world of lies Guide me clearly through that which I dont understand Give me strength to find the path Help me fight any demons as you flow through me wholely This is my prayer, that you protect me from evil, and bring me closer to peace And open up my eyes, so i can see things as you do Amen
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discountpass
Stranger

Registered: 02/08/06
Posts: 160
Loc: SE Florida
Last seen: 10 years, 9 months
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Re: Myecelium dead. :( Can i re-pressure cook and re-inoculate? [Re: SDP]
#5486036 - 04/06/06 10:31 AM (17 years, 9 months ago) |
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might need some air exchange.
-------------------- ---------------------------------------------- Enjoy life, alas! Enjoy life! As death is only the begining.
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SDP
ChronicAficionado



Registered: 01/21/05
Posts: 1,297
Last seen: 5 months, 28 days
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Re: Myecelium dead. :( Can i re-pressure cook and re-inoculate? [Re: discountpass]
#5486073 - 04/06/06 10:43 AM (17 years, 9 months ago) |
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Quote:
discountpass said: might need some air exchange.
Jars (with no tape or foil over holes) in a tupperware with lid on, with about 15 or so 1/3 inch holes across the top under the lid (aliens incubator). That tupperware sits in water in a bigger tupperware that has a water heater heated now to 80 degrees...
Im going to try drilling holes in the top of the big one too for optimal air flow.
Sound iffy?
-SDP
-------------------- Teonanacatl, open up my eyes This sacrament, this prayer, beyond the world of lies Guide me clearly through that which I dont understand Give me strength to find the path Help me fight any demons as you flow through me wholely This is my prayer, that you protect me from evil, and bring me closer to peace And open up my eyes, so i can see things as you do Amen
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Akamatsu
Seeker

Registered: 01/29/06 
Posts: 285
Loc: Australia
Last seen: 2 years, 1 month
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Re: Myecelium dead. :( Can i re-pressure cook and re-inoculate? [Re: SDP]
#5486117 - 04/06/06 10:54 AM (17 years, 9 months ago) |
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The dead mycelium would have used up a portion of the jars nutrients, which could starve new growth. Also, as it decays, the dead mycelium will alter the PH balance and may make the jars more susceptible to contamination. You could try, but you'd probably just be wasting spores.
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SDP
ChronicAficionado



Registered: 01/21/05
Posts: 1,297
Last seen: 5 months, 28 days
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Re: Myecelium dead. :( Can i re-pressure cook and re-inoculate? [Re: Akamatsu]
#5486175 - 04/06/06 11:09 AM (17 years, 9 months ago) |
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Quote:
Akamatsu said: The dead mycelium will alter the PH balance and may make the jars more susceptible to contamination. You could try, but you'd probably just be wasting spores.
Lame... but i think in my heart i know your right. hehe. 
Thanks!
-SDP
-------------------- Teonanacatl, open up my eyes This sacrament, this prayer, beyond the world of lies Guide me clearly through that which I dont understand Give me strength to find the path Help me fight any demons as you flow through me wholely This is my prayer, that you protect me from evil, and bring me closer to peace And open up my eyes, so i can see things as you do Amen
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