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InvisibleFreedomFight
Strange

Registered: 07/03/05
Posts: 427
LC is growing soooo slow
    #4506131 - 08/07/05 05:00 AM (15 years, 2 months ago)

What would you think if 6 days after inoculation into a baby bottle growth is questionable... It kinda looks a little cloudy but could just be the karo. At least it isn't contaminated but 6 days and hardly any growth, is this possible?


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I do not grow anything illegal.
I do not sell anything.
I am, however, a very curious individual.
I also try to be helpful.


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Offlinehawksapprentice
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Re: LC is growing soooo slow [Re: FreedomFight]
    #4506188 - 08/07/05 05:38 AM (15 years, 2 months ago)

Have you done liquid cultures before? What temp. are you incubating it at? What kind of Karo recipe did you follow. How did you inoculate it? i.e. spores or living mycellium.


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"I celebrate the Earth, my home, my mother, my grave, and as long as men are Man they must, if they would preserve the integrated being, do the same---[and preserve]--this rank casual hungry smelly sweaty lusting transitory body, my oozy pulpy liquid-bag-swollen body, bones, blood, hair glands, my bejeweled sex; I love and celebrate it all.  never to let men forget that they are animals as much as gods---that is one thing I shall say."

  Edward Abbey


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Offlinehawksapprentice
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Re: LC is growing soooo slow [Re: hawksapprentice]
    #4509465 - 08/08/05 04:12 AM (15 years, 2 months ago)

Must not want an awnser.


--------------------
"I celebrate the Earth, my home, my mother, my grave, and as long as men are Man they must, if they would preserve the integrated being, do the same---[and preserve]--this rank casual hungry smelly sweaty lusting transitory body, my oozy pulpy liquid-bag-swollen body, bones, blood, hair glands, my bejeweled sex; I love and celebrate it all.  never to let men forget that they are animals as much as gods---that is one thing I shall say."

  Edward Abbey


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InvisibleFooManM
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Registered: 02/02/05
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Re: LC is growing soooo slow [Re: FreedomFight]
    #4509669 - 08/08/05 06:50 AM (15 years, 2 months ago)

The Karo should be clear, that's why it is used for LC's. If you see a cloud, it's probably myc forming. If you have read other posts with people talking about LC's, then you know that it is a SLOW process. However, once you do have your LC ready, it makes up for time lost with the speed of colonization.


--------------------

Quick WBS Prep


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InvisibleFreedomFight
Strange

Registered: 07/03/05
Posts: 427
Re: LC is growing soooo slow [Re: FooMan]
    #4510252 - 08/08/05 01:39 PM (15 years, 2 months ago)

Sorry, I thought I did respond earlier:

it was about 3-4% Karo with multispore inoc. Done in a glovebox. Incubation temps are 82-84F. It is very odd because AFOF have an LC of 2 different strains but no growth in either of them still. The grain jars have been going slow too though but this is pretty sketch now that nothing is growing in the LCs


--------------------
I do not grow anything illegal.
I do not sell anything.
I am, however, a very curious individual.
I also try to be helpful.


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InvisibleFooManM
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Re: LC is growing soooo slow [Re: FreedomFight]
    #4510312 - 08/08/05 02:00 PM (15 years, 2 months ago)

Be patient. LC's usually take AT LEAST a full week to start showing signs of growth. Don't get worried yet.


--------------------

Quick WBS Prep


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Offlineshroomballa
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Registered: 03/26/06
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Loc: Montana
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Re: LC is growing soooo slow [Re: FooMan]
    #5485044 - 04/06/06 01:14 AM (14 years, 6 months ago)

Mine showed slight signs of growth after just 2 days...honey method though.


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Offlinemuskrat
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Registered: 01/25/06
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Re: LC is growing soooo slow [Re: shroomballa]
    #5485233 - 04/06/06 02:22 AM (14 years, 6 months ago)

If you have them in the baby bottles (as per agar's tek), it might just be that there is a lack of air exchange.


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Invisiblecappa
Nerd
Registered: 02/12/06
Posts: 854
Re: LC is growing soooo slow [Re: muskrat]
    #5485350 - 04/06/06 03:33 AM (14 years, 6 months ago)

My average time to see myc growth is around 3 days.

Make sure you have a little air exchange, it seems to help. I use about an 1/8" hole on my pint jars covered with 2 layers of micropore tape.

Cloudy Karo makes me think you might have carmelized your LC. How long did you cook it for etc?


--------------------
Their are 10 types of people. Those that understand binary, and those who don't.
~Cappa.


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