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Shop: North Spore Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies   Mushroom-Hut Liquid Cultures

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Offlinekrnfan145970
Smart commentgoes here
Male
Registered: 08/17/05
Posts: 112
Last seen: 17 years, 6 months
Used syringe
    #5479848 - 04/04/06 04:58 PM (17 years, 9 months ago)

How long do I boil a used syringe to sterilize? I will be using it to draw up LC. Thanks


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Invisiblemusher_420
Stranger

Registered: 08/01/05
Posts: 2,691
Re: Used syringe [Re: krnfan145970]
    #5479872 - 04/04/06 05:08 PM (17 years, 9 months ago)

If you have a PC I would PC the syringe for about 20 minutes.

If you don't have a PC. You can microwave it. I have done this and it worked fine. I would just fill the syringe up with 1cc of water and pull the plunger all the way back. Nuke it until steam was shooting out the end. Then I would let it sit a little bit and repeat a couple times. Finally storing the syringe in a ziplock bag.


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OfflineWronguy
Registered: 03/05/05
Posts: 4,450
Last seen: 12 years, 7 months
Trusted Cultivator
Re: Used syringe [Re: krnfan145970]
    #5479883 - 04/04/06 05:13 PM (17 years, 9 months ago)

I use a 140ML syringe to aspirate my liquid cultures and do the following:

1. Have a pot of boiling water ready to go on the stove.
2. Fold a paper towel into fours and soak one side with alcohol.
3. Aspirate boiling water into the syringe and expel. Do this 3 times.
4. After the last boiling water expulsion, allow the syringe to cool with the needle resting inside the alcohol soaked towel.
5. Spray another paper towel and cover the remainder of the syringe until it has cooled, about 5 minutes, and ready for use.

I've got 16 spawn bags under my belt using the same syringe/needle and the same procedure. It works.


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OfflineDaCultivater
Stranger
Registered: 10/08/05
Posts: 289
Last seen: 15 years, 9 months
Re: Used syringe [Re: Wronguy]
    #5479913 - 04/04/06 05:27 PM (17 years, 9 months ago)

i do that exact thing but instead of the alchahol soaked paper towel i just fill the syringe all the way with the boiling water the last time and then spray i little of the boiling water through the needle with the cap on and then i put it if the fridge for like 15 min. when time comes to use lc, just have an empy glass (cleaned with alchahol) and just spray the steril water into the glass then flame the needle and suck up the lc and so on.

and thats what i do when i use lc (never had a contaminated lc or lc syringe, i have save them for over 3 months)


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as they said in some movie(i don't remember which one);

a belief is a dangerous thing, people will kill over it; instead of believing you need to find your facts and then have an opinionated idea that you are willing to change in light of new evidence


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