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b0ngman
Stranger
Registered: 10/31/05
Posts: 64
Last seen: 11 years, 5 months
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liquid culture directly to poo???
#5478920 - 04/04/06 12:19 PM (17 years, 9 months ago) |
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IF I could do this, I could inject about 100cc into a 5lb tub and it would probably colonize rather quickly. Can I inject mycelium from a liquid culture directly into 50/50 horse/straw?
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Snaggletooth
Stranger in a Strange Land


Registered: 10/24/05
Posts: 6,109
Loc: blinks stupidly
Last seen: 6 years, 8 months
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Re: liquid culture directly to poo??? [Re: b0ngman]
#5478991 - 04/04/06 12:43 PM (17 years, 9 months ago) |
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Yes it is possible but not exactly like your thinking.
Now that it is possible, but it has a higher fail rate, unless you want to experiment, play around, it has no useful application.
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thenewguy05
The Mushroom Man


Registered: 02/11/05
Posts: 2,123
Loc: My Underground Layer
Last seen: 2 years, 5 months
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Re: liquid culture directly to poo??? [Re: b0ngman]
#5478993 - 04/04/06 12:44 PM (17 years, 9 months ago) |
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you need spawn first. otherwise i would be doing it too.
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b0ngman
Stranger
Registered: 10/31/05
Posts: 64
Last seen: 11 years, 5 months
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Re: liquid culture directly to poo??? [Re: thenewguy05]
#5479015 - 04/04/06 12:48 PM (17 years, 9 months ago) |
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why do you need spawn first and why does it have a higher failure rate?
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Snaggletooth
Stranger in a Strange Land


Registered: 10/24/05
Posts: 6,109
Loc: blinks stupidly
Last seen: 6 years, 8 months
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Re: liquid culture directly to poo??? [Re: b0ngman]
#5479048 - 04/04/06 12:53 PM (17 years, 9 months ago) |
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Spawn like WBS, once it has colonized is somewhat resistance to contaminates.
When you spawn it to like hpoo you have hundreds if not thousands of inoculation points.
Then the mycelium can run through the hpoo fairly fast, and the faster it colonizes the less it is susceptible to contaminates.
And the WBS and nuts.....
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thenewguy05
The Mushroom Man


Registered: 02/11/05
Posts: 2,123
Loc: My Underground Layer
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Re: liquid culture directly to poo??? [Re: b0ngman]
#5479052 - 04/04/06 12:56 PM (17 years, 9 months ago) |
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spawn is already establisted culture.
with high spawn rates you shouldn't have a higher failure rate.
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BlueBruiser
Psilocin Technician

Registered: 03/14/02
Posts: 225
Loc: Mushroom planet
Last seen: 3 years, 11 months
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Re: liquid culture directly to poo??? [Re: b0ngman]
#5479196 - 04/04/06 01:25 PM (17 years, 9 months ago) |
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The thing is that the mycelium grows best on grains and such. It doesnt grow very well on just straight horse/straw. What I like to do is use spawn bags. I mix my horse/straw with some rye berries in a spawn bag, steriize, then inoculate with LC, shake well and incubate. I have had 95% colinization of an entire spawn bag in 4 days, thanks to the LC and skipping the spawn stage. Its amazing, give it a try if u can.
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4skin
Azurescens Pimp


Registered: 03/11/06
Posts: 46
Last seen: 17 years, 9 months
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Re: liquid culture directly to poo??? [Re: BlueBruiser]
#5479298 - 04/04/06 01:49 PM (17 years, 9 months ago) |
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Let's see some pics of that spawn bag and a tec for it!
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Mezcal
Registered: 08/11/05
Posts: 1,980
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Re: liquid culture directly to poo??? [Re: 4skin]
#5479399 - 04/04/06 02:24 PM (17 years, 9 months ago) |
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Yeah, I'd like to hear more too...
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Wronguy
Registered: 03/05/05
Posts: 4,450
Last seen: 12 years, 7 months
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Re: liquid culture directly to poo??? [Re: b0ngman]
#5479793 - 04/04/06 04:33 PM (17 years, 9 months ago) |
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Inoculating manure/straw with a liquid culture is spawning. You're taking one colonized medium and introducing it to another substrate. The problem you have is manure doesn't do a very good job at holding moisture and straw is highly susceptible to contaminates.
Your train of thought is where it needs to be, but your substrate would be lacking. I use spawn bags exclusively and inoculate them with liquid cultures. I use a nutritionally diverse bulk substrate and have never lost single bag to a contaminate. The tek you want to read up on is from Blue Helix. It can be found here.
It works very well people.
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baldtim
Making Flippy Floppy



Registered: 01/16/13
Posts: 95
Loc: Iowa, USA
Last seen: 3 years, 11 months
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Re: liquid culture directly to poo??? [Re: Wronguy]
#18433156 - 06/17/13 06:01 PM (10 years, 7 months ago) |
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I'm new to this art. But am enjoying! What I wonder is why I can't take a tub... put 10 pounds of cow poop in it and splash with pan cyan LC and wait for mama?
If I need to pasteurize the poop, I could ... I could keep the poop at a certain temp and humidity... I could vary these... or not...
Then, after it is fully colonized -- may take a while, I know -- I feed it to other tubs of poop/coir/etc. for fruiting. I keep mama satisfied, too.
My understanding is that this is somewhat how it is done in nature. The LC speeds things up... and grains speed things up, too.
I currently have composted cow poop that doesn't have a speck of fuzz or blue anything on it.
Opinions? I thank all of you for the knowledge you've given to me. Tim
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WolfieNuke
Professional Psychonaut



Registered: 12/21/09
Posts: 421
Loc: Washington, US
Last seen: 6 years, 7 months
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Re: liquid culture directly to poo??? [Re: baldtim]
#18433220 - 06/17/13 06:18 PM (10 years, 7 months ago) |
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Yes, this is done in nature. That said, nature has a very high failure rate... If you go hunting, you will notice that there are no pastures completely covered end to end in mushrooms, and the ones that are there were probably quite lucky. You can do what nature does, or you can read and listen to some of the most trusted cultivators here and improve upon nature giving this amazing fungus the most optimal chance to grow and mature.
A month from germination to fruiting is very quick IMHO. Shortcuts and impatience mainly lead to failure in this respect. One thing I have learned in my life has really helped me here:
Slow is Fast...
Try to rush through thing will usually ensure you have to do it all over again. Try to do it right the first time.
Edited by WolfieNuke (06/17/13 06:23 PM)
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baldtim
Making Flippy Floppy



Registered: 01/16/13
Posts: 95
Loc: Iowa, USA
Last seen: 3 years, 11 months
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Re: liquid culture directly to poo??? [Re: WolfieNuke]
#18433649 - 06/17/13 07:54 PM (10 years, 7 months ago) |
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I'm patient. Mycology has taught me to be very patient. Very patient indeed.
I am a novice, speculating. I'm open to experimenting and failing many times over. I'm not thinking a month... just thinking. Reading everything I can... hope to contribute useful info at some point.
Thanks for your response, Tim
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,809
Loc: Canada
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Re: liquid culture directly to poo??? [Re: baldtim]
#18433740 - 06/17/13 08:10 PM (10 years, 7 months ago) |
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RogerRabbit
Bans for Pleasure



Registered: 03/26/03
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Loc: Seattle
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Re: liquid culture directly to poo??? [Re: b0ngman]
#18434478 - 06/17/13 10:37 PM (10 years, 7 months ago) |
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Quote:
b0ngman said: IF I could do this, I could inject about 100cc into a 5lb tub and it would probably colonize rather quickly. Can I inject mycelium from a liquid culture directly into 50/50 horse/straw?
No.
It has somewhat near a zero chance of success. You need the colonized grains as a 'jumping off' point into the straw or manure. If you're sterilizing the straw/manure combo you could put some brf into it and then inoculate with LC, but you'd have to keep it all sterile until full colonization. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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