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spiritualised
Stranger
Registered: 03/28/06
Posts: 1
Last seen: 17 years, 10 months
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Annoying green contamination from offset
#5476354 - 04/03/06 07:59 PM (17 years, 10 months ago) |
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Right this problem is really starting to annoy me and i'm hoping that some wise people here can point me in the right direction.
I've been attempting to grow using the pftek for simple minds method. First attempt: five half pint glasses, concocted using quality 2mm vermiculite, distilled water, home ground brown rice flour, double layer foil lids with prof. grade masking tape over the top in a cross to stop the foil ripping when innoculating, another layer over the top. The jars were steam sterilised for 90 mins and left overnight to cool. I inoculated the next day sterilising the needle in an alcohol flame. Within five days of inoculation the very first signs of white appeared but immediately turned green in all five glasses. After leaving for a week, I binned the contents, left the glasses in a bucket of bleach, then boiled them.
2nd attempt Identical to first, however, purchased disposable gloves, pre sterilised the flour & vermiculite in the oven at 350F for 20 mins, sterilised the entire working surface, jars, all utensils, gloves & foil with isopropyl alcohol. Whilst filling the glasses, wiped the top cm down with alcohol then boiled the prepped glasses for two hours. inoculated the next day with flame sterilisation of the whole needle between each glass.
Same result. Immediate green, very sporadic patches of white in a sea of green.

The speed in which they contaminate makes me suspicious of the syringe, which was purchased from a local head shop. Anyone else think this is the case or am I doing something complete wrong.
Thanks in advance
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RogerRabbit
Bans for Pleasure


Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 11 months, 21 days
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Re: Annoying green contamination from offset [Re: spiritualised]
#5478528 - 04/04/06 09:53 AM (17 years, 10 months ago) |
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Agreed. Sounds like a contaminated syringe. However, there is no benefit to cooking your substrate in the oven. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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Sinthetic
Stranger


Registered: 12/05/05
Posts: 812
Last seen: 15 years, 7 months
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Re: Annoying green contamination from offset [Re: RogerRabbit]
#5478775 - 04/04/06 11:22 AM (17 years, 10 months ago) |
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Here's an idea. Do another batch but don't inoculate one of them. If all others contam start ordering syringes from shroomery sponsors lol.
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