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Offlinevegitative
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Registered: 01/21/06
Posts: 97
Last seen: 17 years, 7 months
LC Question
    #5475382 - 04/03/06 04:17 PM (17 years, 9 months ago)

After a lot of reading about LCs it looks like that's the way to go for someone like me, who doesn't have the money to back up buying spore syringes consistently, and wants the fast colonization and all that...

So a quick question about LC, I've seen a lot of ways to get the culture out and all, but hypothetically speaking, using a very small jar (one of those 1/4 pint canning jars that are like 2 inches tall), could you put some honey (4% of the full jar, with some air space) in the bottom, put a syringe made from a print into the jar, depress the syringe completely into the jar and then leave it. Some water would probably have to be in it ahead of time, but since I use sterile techniques to make spore prints into syringes, could I just leave the syringe in the jar and then, when the lc was done, suck it all back in.

No worries about contaminations there since it always stays sealed... I'm sure someone's come up with this before but what are the options here?


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OfflineSolidcell
tolerance++;
Male

Registered: 01/20/06
Posts: 754
Loc: Memphis & 53rd
Last seen: 15 years, 4 months
Re: LC Question [Re: vegitative]
    #5475499 - 04/03/06 04:37 PM (17 years, 9 months ago)

LCs are useful because you can make many syringes out of just one (if u use a pint jar or bigger). But if you honestly want just that little bit of LC (waste of time/energy IMO), then yea I could see that working. You still ahve to sterilize the honey and jar, so I'd plan out how much water is going into the jar from the syringe, and add the rest of the water you'll need to the honey and then sterilize. I wouldn't sterilize just honey in the jar though, I would imagine it would carmalize easier.


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