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OfflineACN45
Stranger
Registered: 11/28/05
Posts: 160
Last seen: 17 years, 9 months
Honey Liquid Culture
    #5474685 - 04/03/06 01:54 PM (17 years, 9 months ago)

All i have is organic honey. Can someone give me a good honey tek besides cdubbs999 or whoever. His really sucks its just a generic here do this. I need details because i cant be wasting any spores. Im concerened with the details like if i need tyvek and how to inncolulate it with the least possible contams.


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Offlinethenewguy05
The Mushroom Man
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Registered: 02/11/05
Posts: 2,123
Loc: My Underground Layer
Last seen: 2 years, 5 months
Re: Honey Liquid Culture [Re: ACN45]
    #5474698 - 04/03/06 01:58 PM (17 years, 9 months ago)

96 grams/ml of water and 4 grams/ml of honey. mix and pressure cook for 20 minutes at 15 psi.

of course you will need a filter of some sort.

least possible contams you want a glove box.


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Invisiblefee
Im he who is the
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Registered: 01/16/03
Posts: 18,238
Loc: amsterdam
Re: Honey Liquid Culture *DELETED* [Re: thenewguy05]
    #5474711 - 04/03/06 02:02 PM (17 years, 9 months ago)

Post deleted by fee

Reason for deletion: ...



--------------------

blankk said to fee:
btw you're a total fucking psychedelic pimp
Turtletotem said:
I want to become a sun worshipper, so next time an atheist smugly asks me where god is, I can point smugly at the sun and laugh my ass off.

Then I drive away in my solar powered piece of shit car, cool stuff man.

And then I go kill a bitch because the flaming orb in the sky told me to do so, and I don't know, oppress a few minorities here and there in the name of nuclear fusion?

Religion is fun.


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OfflineACN45
Stranger
Registered: 11/28/05
Posts: 160
Last seen: 17 years, 9 months
Re: Honey Liquid Culture [Re: fee]
    #5474938 - 04/03/06 02:47 PM (17 years, 9 months ago)

do you have any links. Im confused as the procedure while innoculating. What ive taken so far is that you have a tyvek around the jar, then the container with a drilled hole stuffed with polyfil. Is that basically correct? There will be enough oxygen getting to the mycelium?


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OfflineSolidcell
tolerance++;
Male

Registered: 01/20/06
Posts: 754
Loc: Memphis & 53rd
Last seen: 15 years, 4 months
Re: Honey Liquid Culture [Re: ACN45]
    #5474952 - 04/03/06 02:50 PM (17 years, 9 months ago)

look up agar's LC lid. you don't want tyvek with a LC.


--------------------
Psychedelic Jar Project

"It is dangerous to be right when the government is wrong." -Voltaire

"Everything that I accepted as being true up to now I acquired from the senses or through the senses. However, I have occasionally found that they deceive me, and it is prudent never to trust those who have deceived us, even if only once." -Descartes


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OfflineAsc1189
The New Guy

Registered: 11/18/05
Posts: 62
Last seen: 12 years, 10 months
Re: Honey Liquid Culture [Re: Solidcell]
    #5475129 - 04/03/06 03:36 PM (17 years, 9 months ago)

Ive been using the tape agar uses. Its great. You tape up both drilled holes. Before yo inoculate wipe down needle with alcohol then poke throught one of the holes and then retape the same hole you inoculated. Then cover with with a couple of coffee filters then rubberband. You can use the tyvek but the tape has never failed me yet.


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OfflineShroomsnotWar
Enthusiast
Male

Folding@home Statistics
Registered: 03/27/06
Posts: 50
Last seen: 15 years, 11 months
Re: Honey Liquid Culture [Re: ACN45]
    #5475161 - 04/03/06 03:42 PM (17 years, 9 months ago)

LC lids that don't leak

Agar's LC lids, I made some for my recent LC's. Works great, and it's real easy to put together.


--------------------
"Last time I checked, I was paying your salary" - me, to a cop, Feb 2000 ... yes I was arrested


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Invisiblecappa
Nerd
Registered: 02/12/06
Posts: 854
Re: Honey Liquid Culture [Re: Asc1189]
    #5475164 - 04/03/06 03:43 PM (17 years, 9 months ago)

pint jar. drill 1/8" hole near the edge. Place micropore tape over it.

add:
a 1" sqaure rock or marble or steel bolt.
1 cup water.
1 small tsp of honey

PC 20minutes @ 15PSI.

Let cool COMPLETELY.

Flame spore needle RED HOT. wrap in alcohol soaked pad.

Insert needle into pint jar through the micropore tape that is covering the 1/8" hole.

Push plunger to release desired amount of spores(2cc's is fine).

Remove needle from jar, place another layer of micropore tape over the hole.

Incubate LC.

It's not rocket science. Just start giving it a try.


--------------------
Their are 10 types of people. Those that understand binary, and those who don't.
~Cappa.


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OfflineAkamatsu
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Registered: 01/29/06 Happy 18th Shroomiversary!
Posts: 285
Loc: Australia
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Re: Honey Liquid Culture [Re: cappa] * 1
    #5475191 - 04/03/06 03:46 PM (17 years, 9 months ago)

_Otto_ made a very easy to follow pictorial, have a look at A *beginner friendly* method for making Liquid Culture.


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OfflineACN45
Stranger
Registered: 11/28/05
Posts: 160
Last seen: 17 years, 9 months
Re: Honey Liquid Culture [Re: cappa]
    #5475313 - 04/03/06 04:06 PM (17 years, 9 months ago)

would a bandaid work instead of micropore tape?


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Invisiblecappa
Nerd
Registered: 02/12/06
Posts: 854
Re: Honey Liquid Culture [Re: ACN45]
    #5475592 - 04/03/06 05:05 PM (17 years, 9 months ago)

yup. It's been tried and works fine. A friend of mine uses the square ones with the sticky all the way around it.


--------------------
Their are 10 types of people. Those that understand binary, and those who don't.
~Cappa.


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Offlinemogur
regnartS

Registered: 11/15/05
Posts: 322
Loc: Puget Sound
Last seen: 11 years, 2 months
Re: Honey Liquid Culture [Re: cappa]
    #5475747 - 04/03/06 05:45 PM (17 years, 9 months ago)

And, they finally came out with silver treated bandaids, which helps keep contams at bay.


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Invisiblecappa
Nerd
Registered: 02/12/06
Posts: 854
Re: Honey Liquid Culture [Re: mogur]
    #5475768 - 04/03/06 05:51 PM (17 years, 9 months ago)

I'll be damned! Learn somethin new every day!


--------------------
Their are 10 types of people. Those that understand binary, and those who don't.
~Cappa.


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Offlineiamyour_messiah
Stranger

Registered: 03/07/06
Posts: 273
Last seen: 4 years, 1 month
Re: Honey Liquid Culture [Re: cappa]
    #5511340 - 04/13/06 07:40 AM (17 years, 9 months ago)

If a band-aid can be used in this case (and I assume you mean the beige colored sticky part of the band-aid with the real tiny holes all over it...) could that be used on grain/pf jars as well instead of polyfill or tyvek?


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