Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Original Sensible Seeds Autoflowering Cannabis Seeds, Bulk Cannabis Seeds   Mushroom-Hut Liquid Cultures   North Spore Bulk Substrate, Injection Grain Bag

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
InvisibleDT2K
asdsadwvwv
Registered: 10/21/05
Posts: 69
Illogical colonization.
    #5471229 - 04/02/06 04:14 PM (17 years, 9 months ago)

Say I have 20 grow bags full of substrate. The substrate consists of:

-basmati organic brown rice
-quinoa
-millet
-flax seed
-vermiculite

The ingredients were initially soaked for 24 hours, then cooked together for an hour (all except vermiculite, obviously). Then this substrate was placed into grow bags and pressure cooked for 3 hours at 15psi. After letting cool for 24 hours, the bags were inoculated with 140cc of liquid culture.

The liquid culture consisted of malt extract and water, which was left to colonize for 2.5 weeks with daily shakings.

A week after the inoculation, I noticed that 5 of my bags took off and were almost done, 4 were somewhat in the middle and undecided and 2 were contaminated. The remaining 9 were completely void of any growth, be it mycelium or contaminants, which is what I don't understand.

What causes a bag which is almost identical in all respects to its neighbours to not take?

A list of reasons or any suggestions would be greatly appreciated.


Extras: Filter Print Post Top
OfflineMerchantP
Stranger
Registered: 03/13/06
Posts: 58
Last seen: 16 years, 10 months
Re: Illogical colonization. [Re: DT2K]
    #5471573 - 04/02/06 05:43 PM (17 years, 9 months ago)

no idea in your situation, but in mine my friend prepared 4 jars and I prepared 4, the only difference in our methods seemed to be he packed his substrate and I made my substrate loose in the jar. My 4 were the only 4 that colonized.


Edited by MerchantP (04/02/06 05:44 PM)


Extras: Filter Print Post Top
InvisibleDT2K
asdsadwvwv
Registered: 10/21/05
Posts: 69
Re: Illogical colonization. [Re: MerchantP]
    #5471777 - 04/02/06 06:30 PM (17 years, 9 months ago)

No, the bags are very airy. The problem is that some colonized and some didn't, even though they were identical.


Extras: Filter Print Post Top
OfflineHongKongPhooey
Stranger
 User Gallery

Registered: 01/17/06
Posts: 172
Last seen: 10 years, 5 months
Re: Illogical colonization. [Re: DT2K]
    #5471791 - 04/02/06 06:33 PM (17 years, 9 months ago)

i take it you prepared all the bags ingredients together? you might as well try inoculating it again if its not doing anything


Extras: Filter Print Post Top
InvisibleDT2K
asdsadwvwv
Registered: 10/21/05
Posts: 69
Re: Illogical colonization. [Re: HongKongPhooey]
    #5471818 - 04/02/06 06:40 PM (17 years, 9 months ago)

The moisture content is almost identical in all the bags. And yea, each 140cc syringe had ALOT of liquid culture in the malt solution. Although re-innoculating might fix these bags (even though I believe that having 280cc's of liquid standing in a bag will screw me royally), my concern is how do I prevent this random colonization in the future. Could it be that some of the liquid mycelium was already dead since I did leave it colonizing for almost 3 weeks?(which is abit long)


Extras: Filter Print Post Top
Invisiblefee
Im he who is the
 User Gallery

Registered: 01/16/03
Posts: 18,238
Loc: amsterdam
Re: Illogical colonization. *DELETED* [Re: DT2K]
    #5471836 - 04/02/06 06:43 PM (17 years, 9 months ago)

Post deleted by fee

Reason for deletion: bla



--------------------

blankk said to fee:
btw you're a total fucking psychedelic pimp
Turtletotem said:
I want to become a sun worshipper, so next time an atheist smugly asks me where god is, I can point smugly at the sun and laugh my ass off.

Then I drive away in my solar powered piece of shit car, cool stuff man.

And then I go kill a bitch because the flaming orb in the sky told me to do so, and I don't know, oppress a few minorities here and there in the name of nuclear fusion?

Religion is fun.


Extras: Filter Print Post Top
OfflineEquilibriuM
dream stalker

Registered: 07/17/05
Posts: 2,323
Last seen: 16 years, 7 months
Re: Illogical colonization. [Re: fee]
    #5471890 - 04/02/06 06:56 PM (17 years, 9 months ago)

I think your bags are a little too wet. use more vermiculite next time. 140 ml is quite a bit of liquid the bag needs to be a little dryer then normal to accept that much LC. Wronguy has a very good bag recipe if you want it, I have it saved...


--------------------
HELP!!!!!!!!!


Extras: Filter Print Post Top
OfflineCutter
other side guy
Male User Gallery

Registered: 01/27/06
Posts: 108
Loc: Oh Yeah land
Last seen: 3 years, 9 months
Re: Illogical colonization. [Re: fee]
    #5471936 - 04/02/06 07:06 PM (17 years, 9 months ago)

I'd say get a nice Simco ionizer to help prevent contamination, and I would expect that it is also being packed to tight in some bags when shaking, maybe use a courser vermiculite, or a perlite to chunk it up.

140cc's seems like a overkill, try 20cc's in one bag next time, then one bag with 40 cc's and one with 80cc's and monitor the progression differences.

What kind of contamination are you getting?

I would shake after inoculation and let it sit for about a week before shaking it again.

Shaking it everydsay seems like a overkill, and might be the problem.


Extras: Filter Print Post Top
InvisibleDT2K
asdsadwvwv
Registered: 10/21/05
Posts: 69
Re: Illogical colonization. [Re: Cutter]
    #5472072 - 04/02/06 07:55 PM (17 years, 9 months ago)

First off, the shaking every day was regarding the liquid mycelium, not the bags. The bags I shook once right after inoculation, and again 5 days later.

The few contaminated ones I had looked like black mycelium culture fully covering all the rice. Basically, they looked like the normal finished cakes are supposed to except they were black, hard and somewhat wet.

Also, 20cc's per pag seems like far too few for the size of the bags I'm using. 20cc's is roughly the quantity youd use on 3-5 250ml jars, one bag has roughly 25 jars worth of substrate. But it might be that the bags are too wet, although, they REALLY dont look it, they look just fine. Moreover, why did some bags take so well while others not? They both got the same amount of innoculant.

Thanks for the help so far though, I am considering the moisture from innoculant as a serious possibility now.


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Original Sensible Seeds Autoflowering Cannabis Seeds, Bulk Cannabis Seeds   Mushroom-Hut Liquid Cultures   North Spore Bulk Substrate, Injection Grain Bag


Similar ThreadsPosterViewsRepliesLast post
* Re: --Using mylecuim to colonize more substrate?-- sutec 2,091 9 01/11/00 04:17 PM
by kbone
* No vermiculite! Substrate, peroxide and sterility? shaganoz 2,526 1 05/11/02 04:05 PM
by Anno
* Honey Water + Rye Flour/Vermiculite Substrate Split 2,951 5 01/07/02 03:17 PM
by Split
* 100% vermiculite substrate? Stainmeblue 1,763 2 08/23/02 11:14 AM
by apatheticgoddess
* adding lite to colonizing dung substrate PSILOCYBE_RICKY 865 3 09/02/02 09:08 PM
by Shaw
* rye colonizing. bulk substrate ? walt 5,009 5 07/10/02 03:14 AM
by walt
* Re: Mixing substrate with casing! The next big thing?
( 1 2 all )
LinkA 6,241 21 01/15/00 03:52 PM
by Anonymous
* Top COLONIZED Bottom ISN'T KaliedoscopEyes 1,364 5 08/19/02 10:48 AM
by KaliedoscopEyes

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
557 topic views. 30 members, 186 guests and 36 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.028 seconds spending 0.011 seconds on 14 queries.