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DT2K
asdsadwvwv
Registered: 10/21/05
Posts: 69
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Illogical colonization.
#5471229 - 04/02/06 04:14 PM (17 years, 9 months ago) |
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Say I have 20 grow bags full of substrate. The substrate consists of:
-basmati organic brown rice -quinoa -millet -flax seed -vermiculite
The ingredients were initially soaked for 24 hours, then cooked together for an hour (all except vermiculite, obviously). Then this substrate was placed into grow bags and pressure cooked for 3 hours at 15psi. After letting cool for 24 hours, the bags were inoculated with 140cc of liquid culture.
The liquid culture consisted of malt extract and water, which was left to colonize for 2.5 weeks with daily shakings.
A week after the inoculation, I noticed that 5 of my bags took off and were almost done, 4 were somewhat in the middle and undecided and 2 were contaminated. The remaining 9 were completely void of any growth, be it mycelium or contaminants, which is what I don't understand.
What causes a bag which is almost identical in all respects to its neighbours to not take?
A list of reasons or any suggestions would be greatly appreciated.
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MerchantP
Stranger
Registered: 03/13/06
Posts: 58
Last seen: 16 years, 10 months
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Re: Illogical colonization. [Re: DT2K]
#5471573 - 04/02/06 05:43 PM (17 years, 9 months ago) |
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no idea in your situation, but in mine my friend prepared 4 jars and I prepared 4, the only difference in our methods seemed to be he packed his substrate and I made my substrate loose in the jar. My 4 were the only 4 that colonized.
Edited by MerchantP (04/02/06 05:44 PM)
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DT2K
asdsadwvwv
Registered: 10/21/05
Posts: 69
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Re: Illogical colonization. [Re: MerchantP]
#5471777 - 04/02/06 06:30 PM (17 years, 9 months ago) |
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No, the bags are very airy. The problem is that some colonized and some didn't, even though they were identical.
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HongKongPhooey
Stranger


Registered: 01/17/06
Posts: 172
Last seen: 10 years, 5 months
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Re: Illogical colonization. [Re: DT2K]
#5471791 - 04/02/06 06:33 PM (17 years, 9 months ago) |
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i take it you prepared all the bags ingredients together? you might as well try inoculating it again if its not doing anything
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DT2K
asdsadwvwv
Registered: 10/21/05
Posts: 69
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The moisture content is almost identical in all the bags. And yea, each 140cc syringe had ALOT of liquid culture in the malt solution. Although re-innoculating might fix these bags (even though I believe that having 280cc's of liquid standing in a bag will screw me royally), my concern is how do I prevent this random colonization in the future. Could it be that some of the liquid mycelium was already dead since I did leave it colonizing for almost 3 weeks?(which is abit long)
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fee
Im he who is the


Registered: 01/16/03
Posts: 18,238
Loc: amsterdam
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Re: Illogical colonization. *DELETED* [Re: DT2K]
#5471836 - 04/02/06 06:43 PM (17 years, 9 months ago) |
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Post deleted by feeReason for deletion: bla
--------------------
blankk said to fee: btw you're a total fucking psychedelic pimp Turtletotem said: I want to become a sun worshipper, so next time an atheist smugly asks me where god is, I can point smugly at the sun and laugh my ass off. Then I drive away in my solar powered piece of shit car, cool stuff man. And then I go kill a bitch because the flaming orb in the sky told me to do so, and I don't know, oppress a few minorities here and there in the name of nuclear fusion? Religion is fun.
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EquilibriuM
dream stalker

Registered: 07/17/05
Posts: 2,323
Last seen: 16 years, 7 months
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Re: Illogical colonization. [Re: fee]
#5471890 - 04/02/06 06:56 PM (17 years, 9 months ago) |
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I think your bags are a little too wet. use more vermiculite next time. 140 ml is quite a bit of liquid the bag needs to be a little dryer then normal to accept that much LC. Wronguy has a very good bag recipe if you want it, I have it saved...
-------------------- HELP!!!!!!!!!
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Cutter
other side guy


Registered: 01/27/06
Posts: 108
Loc: Oh Yeah land
Last seen: 3 years, 9 months
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Re: Illogical colonization. [Re: fee]
#5471936 - 04/02/06 07:06 PM (17 years, 9 months ago) |
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I'd say get a nice Simco ionizer to help prevent contamination, and I would expect that it is also being packed to tight in some bags when shaking, maybe use a courser vermiculite, or a perlite to chunk it up.
140cc's seems like a overkill, try 20cc's in one bag next time, then one bag with 40 cc's and one with 80cc's and monitor the progression differences.
What kind of contamination are you getting?
I would shake after inoculation and let it sit for about a week before shaking it again.
Shaking it everydsay seems like a overkill, and might be the problem.
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DT2K
asdsadwvwv
Registered: 10/21/05
Posts: 69
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Re: Illogical colonization. [Re: Cutter]
#5472072 - 04/02/06 07:55 PM (17 years, 9 months ago) |
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First off, the shaking every day was regarding the liquid mycelium, not the bags. The bags I shook once right after inoculation, and again 5 days later.
The few contaminated ones I had looked like black mycelium culture fully covering all the rice. Basically, they looked like the normal finished cakes are supposed to except they were black, hard and somewhat wet.
Also, 20cc's per pag seems like far too few for the size of the bags I'm using. 20cc's is roughly the quantity youd use on 3-5 250ml jars, one bag has roughly 25 jars worth of substrate. But it might be that the bags are too wet, although, they REALLY dont look it, they look just fine. Moreover, why did some bags take so well while others not? They both got the same amount of innoculant.
Thanks for the help so far though, I am considering the moisture from innoculant as a serious possibility now.
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