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Shadowrider
What am "I"....?

Registered: 02/26/06
Posts: 72
Last seen: 9 years, 8 months
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IT's ALivE!
#5470640 - 04/02/06 12:07 PM (17 years, 9 months ago) |
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Hey all, some good news. I took a look into my incubator today and after thinking nothing was going to happen to my surprise some jars showed signs of colonization. I originally made 8 brf jars which where sterilized in a PC and left for about 3 weeks before inoculation. 1 jar was removed so far due to my paranoia with contams. Jars have been shot up with z-strain. After 6 days in the incubator, out of the 7 jars 2 are showing great signs on all shot points, 2 are showing small development signs 3 showing nothing. I noticed my temps a little low 26.5 C so I put a blanket over top of TiT for better insulation. However I wanted to ask are my results on track for 6 days of incubation? Keeping in mind that my jars sat in the PC for 3 weeks after being sterilized. Grow log will be starting soon with pics in a week or so keep an eye out, my mushies will be awsome (at least I hope so).
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Dr_Weird
Medicine Man


Registered: 03/11/06
Posts: 75
Loc: My Laboratory
Last seen: 24 days, 17 hours
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Your temperature is okay, though raising it a few degrees won't hurt. Also make sure the jars are getting at least some air exchange (loosening the lids, uncover pinholes). That is probably why some jars are doing well while others are not. If you did a verm layer that should keep out the contams. Give the 3 nothing jars a few more days & loosen their lids. If still you get nothing, reinoculate.
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Solidcell
tolerance++;


Registered: 01/20/06
Posts: 754
Loc: Memphis & 53rd
Last seen: 15 years, 4 months
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Re: IT's ALivE! [Re: Dr_Weird]
#5471100 - 04/02/06 03:29 PM (17 years, 9 months ago) |
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Try inoculating with liquid cultures next time, you'll love how much faster it goes. I had a weak spore syringe I got in the mail of Z and it took forever to colonize, and with a liquid culture inoculatuion, it's a breeze.
-------------------- Psychedelic Jar Project "It is dangerous to be right when the government is wrong." -Voltaire "Everything that I accepted as being true up to now I acquired from the senses or through the senses. However, I have occasionally found that they deceive me, and it is prudent never to trust those who have deceived us, even if only once." -Descartes
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Shadowrider
What am "I"....?

Registered: 02/26/06
Posts: 72
Last seen: 9 years, 8 months
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Re: IT's ALivE! [Re: Solidcell]
#5471435 - 04/02/06 05:07 PM (17 years, 9 months ago) |
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Thanks for your input guys. I am going to loosen the lids on the 5 slower jars. I have gone and got some pint jars for a WBS try. I will research LC more and maybe give it a try too. For now I'm going to make a syringe from a print I received and try my hand at WBS.
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