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BPJohnny
Stranger


Registered: 03/28/06
Posts: 389
Last seen: 17 years, 7 months
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Think this thing will work??
#5468310 - 04/01/06 05:36 PM (17 years, 9 months ago) |
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Thinking about buying this greenhouse thing for $99 to create a sterile work area in my house think it will work??
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mikeownow
Humungus fungus

Registered: 09/01/05
Posts: 2,856
Loc: WA,USA
Last seen: 17 years, 3 months
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Re: Think this thing will work?? [Re: BPJohnny]
#5468320 - 04/01/06 05:39 PM (17 years, 9 months ago) |
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my bedroom seems to stay prety sterile, No contams in any jars yet
-------------------- No statements made in any post or message by myself should be construed to mean that I am now, or have ever been, participating in or considering participation in any activities in violation of any local, state, or federal laws. All posts are works of fiction.
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BPJohnny
Stranger


Registered: 03/28/06
Posts: 389
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Re: Think this thing will work?? [Re: mikeownow]
#5468332 - 04/01/06 05:44 PM (17 years, 9 months ago) |
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yeah i have done it like that before but i want to start doing culture dishes and need to get an area thats as sterile as possible
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MindsEye
AMT Fiend


Registered: 02/20/06
Posts: 645
Loc: cerebral cortex
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Re: Think this thing will work?? [Re: mikeownow]
#5468333 - 04/01/06 05:45 PM (17 years, 9 months ago) |
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Quote:
mikeownow said: my bedroom seems to stay prety sterile, No contams in any jars yet
 The techniques you use are more important than your work environment,
-------------------- Easy Poo Cake Tek
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ShroomInduced
I read too manymycology books


Registered: 04/19/05
Posts: 309
Loc: A giant mushroom in my he...
Last seen: 12 years, 5 months
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Re: Think this thing will work?? [Re: MindsEye]
#5468398 - 04/01/06 06:07 PM (17 years, 9 months ago) |
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It looks like that thing would work.
-------------------- That's a deep kiss too, like the Europeans. You know, the French, they have to unhinge their jaw to show love. Computer Fan Tek Vote for you favorite strain of cubes 97 choices!
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BPJohnny
Stranger


Registered: 03/28/06
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Re: Think this thing will work?? [Re: ShroomInduced]
#5468433 - 04/01/06 06:16 PM (17 years, 9 months ago) |
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excellent thanks much
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Snaggletooth
Stranger in a Strange Land


Registered: 10/24/05
Posts: 6,109
Loc: blinks stupidly
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Re: Think this thing will work?? [Re: BPJohnny]
#5468534 - 04/01/06 06:36 PM (17 years, 9 months ago) |
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Interesting Idea...
But remember you carry a lot of contaminates on you, all over you head to toe.
Unless you dress appropriate you could be facing problems. And realistically that usually does not happen, every time you go in.
--------------------
Atheist Chat
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Waffle
patty

Registered: 03/05/06
Posts: 289
Last seen: 9 years, 5 months
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Re: Think this thing will work?? [Re: Snaggletooth]
#5468563 - 04/01/06 06:44 PM (17 years, 9 months ago) |
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Do it naked, of course shower first.
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BPJohnny
Stranger


Registered: 03/28/06
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Re: Think this thing will work?? [Re: Waffle]
#5468575 - 04/01/06 06:48 PM (17 years, 9 months ago) |
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yea i know i have to build like a "buffer" area but i still dont know how to go about disinfecting myself
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cappa
Nerd
Registered: 02/12/06
Posts: 854
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Re: Think this thing will work?? [Re: BPJohnny]
#5468637 - 04/01/06 07:08 PM (17 years, 9 months ago) |
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The ancient east american indian tribe 'The Mycocois' used to use the shamanic ritual of 'Death Dancing' to inhibit the birth and growth of unwanted spirits(harmful microoganisms) in their mushroom crops. This ritual often entailed parading young, naked, female women adorned in feathered hats up and down the space in front of their dwellings, while the males smeared corn oil all over the females' bodies.
This was said to 'purify the soul of the mycellium', increase potency, and ward off evil spirits.
-------------------- Their are 10 types of people. Those that understand binary, and those who don't. ~Cappa.
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BPJohnny
Stranger


Registered: 03/28/06
Posts: 389
Last seen: 17 years, 7 months
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Re: Think this thing will work?? [Re: cappa]
#5468696 - 04/01/06 07:26 PM (17 years, 9 months ago) |
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i think i'll try that!
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Snaggletooth
Stranger in a Strange Land


Registered: 10/24/05
Posts: 6,109
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Re: Think this thing will work?? [Re: BPJohnny]
#5468718 - 04/01/06 07:32 PM (17 years, 9 months ago) |
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spray your self down with Lysol
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Atheist Chat
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cappa
Nerd
Registered: 02/12/06
Posts: 854
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Re: Think this thing will work?? [Re: BPJohnny]
#5468725 - 04/01/06 07:34 PM (17 years, 9 months ago) |
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 Glad you liked that!
On a serious note, That greenhouse looks fine to me. Although it's hard to tell if it's airtight etc..
You don't need a 'staging area' or whatever you called it. Just wear freshly laundered clothing that isn't 'fuzzy'. You can hose yourself down with lysol if you like.
Keep ample amounts of alcohol and 5% bleach water inside of the unit, to clean yourself in between proceedures. You'll be fine if you go with your gut feeling about 'How clean am I to a microorganism?'.
-------------------- Their are 10 types of people. Those that understand binary, and those who don't. ~Cappa.
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BPJohnny
Stranger


Registered: 03/28/06
Posts: 389
Last seen: 17 years, 7 months
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Re: Think this thing will work?? [Re: cappa]
#5468761 - 04/01/06 07:39 PM (17 years, 9 months ago) |
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the biggest thing i am afraid of is fabric. Is lysol flammable?? if so
can i use a 10% bleach solution? will it be as effective?
I am worried that i'll douse myself in lysol go to use an alcohol lamp and torch myself
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cappa
Nerd
Registered: 02/12/06
Posts: 854
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Re: Think this thing will work?? [Re: BPJohnny]
#5468780 - 04/01/06 07:43 PM (17 years, 9 months ago) |
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10% bleach is IMO the most effective disinfectant. But you will run into breathing problems using it inside of that greenhouse! Even 10% bleach solution will fuck you up if you breath it's vapors very long. I'd knock it down a knotch or two and go with that.
If you're worried about the fabric, just get some polyester clothes. Then you can look like a disco master while your tending to your crop! haha
-------------------- Their are 10 types of people. Those that understand binary, and those who don't. ~Cappa.
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BPJohnny
Stranger


Registered: 03/28/06
Posts: 389
Last seen: 17 years, 7 months
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Re: Think this thing will work?? [Re: cappa]
#5468794 - 04/01/06 07:46 PM (17 years, 9 months ago) |
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oh yes polyester i never thought of that! great idea! off to goodwill tomorrow for some "lab clothes" hehe
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BPJohnny
Stranger


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Posts: 389
Last seen: 17 years, 7 months
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Re: Think this thing will work?? [Re: BPJohnny]
#5468804 - 04/01/06 07:48 PM (17 years, 9 months ago) |
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as far as breathing goes i want to install a couple HEPA filters in the sides of it for some fresh air
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cappa
Nerd
Registered: 02/12/06
Posts: 854
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Re: Think this thing will work?? [Re: BPJohnny]
#5469180 - 04/01/06 09:16 PM (17 years, 9 months ago) |
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FAE is critical to pin formation and healthy fruit growth. What solution you apply to get it is entirely up to you and needs to be custom to each application.
Filtered ports in the bottom of it may work. Another solution is to implement those AND an exaust fan. When the exaust fan runs, it will push out your warm, co2 enriched, humid air. At the same time it will draw in drier(depending on the air outside the greenhouse), sometimes colder, filtered air. So if you use an exaust fan, you will want your humidifier or coolmist running while the exaust fan is running.
-------------------- Their are 10 types of people. Those that understand binary, and those who don't. ~Cappa.
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BPJohnny
Stranger


Registered: 03/28/06
Posts: 389
Last seen: 17 years, 7 months
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Re: Think this thing will work?? [Re: cappa]
#5469187 - 04/01/06 09:17 PM (17 years, 9 months ago) |
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this is not what im using as a grow chamber is more of a makeshift clean room
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cappa
Nerd
Registered: 02/12/06
Posts: 854
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Re: Think this thing will work?? [Re: BPJohnny]
#5469195 - 04/01/06 09:21 PM (17 years, 9 months ago) |
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bahaha! I got sidetracked reading other threads.. sorry bro.
Ya, that makes sense then. You will want any moisture you may get in there to be able to evaporate and leave the structure.
-------------------- Their are 10 types of people. Those that understand binary, and those who don't. ~Cappa.
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coda
Banjo Goiter


Registered: 03/20/01
Posts: 8,750
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Re: Think this thing will work?? [Re: cappa]
#5469327 - 04/01/06 10:02 PM (17 years, 9 months ago) |
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why not just build a glove box, it will save you about 80 bucks and is a lot easier to work with.
-------------------- To get really high is to forget yourself. And to forget yourself is to see everything else. And to see everything else is to become an understanding molecule in evolution, a conscious tool of the universe. And I think every human being should be a conscious tool of the universe. . . . -JG i really am glad you came back to us instead of taking the other path. *hug* -A_S (RIP your final words to me will never be forgotten)
 Don't fuck with the laughing jesus.
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BPJohnny
Stranger


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Posts: 389
Last seen: 17 years, 7 months
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Re: Think this thing will work?? [Re: coda]
#5469393 - 04/01/06 10:23 PM (17 years, 9 months ago) |
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i thought about that but it just doesn't seem like enough space for culture dishes i think i would feel more comfortable in my "clean room"
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ShroomInduced
I read too manymycology books


Registered: 04/19/05
Posts: 309
Loc: A giant mushroom in my he...
Last seen: 12 years, 5 months
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Re: Think this thing will work?? [Re: BPJohnny]
#5469451 - 04/01/06 10:43 PM (17 years, 9 months ago) |
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Is what I would do with that thing is put a hepa filter in the top rear, have it blowing in at all times, and then cut some vents at the bottom of the tent, this way the air would be pushing all the contams out of the bottom of the tent. Thats how they do it in real labs..
-------------------- That's a deep kiss too, like the Europeans. You know, the French, they have to unhinge their jaw to show love. Computer Fan Tek Vote for you favorite strain of cubes 97 choices!
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BPJohnny
Stranger


Registered: 03/28/06
Posts: 389
Last seen: 17 years, 7 months
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Re: Think this thing will work?? [Re: ShroomInduced]
#5469458 - 04/01/06 10:47 PM (17 years, 9 months ago) |
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great tip! thanks for all the info its really helpful. and by all means keep it comin!
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d4a2n0k
The Dude


Registered: 07/23/03
Posts: 742
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Re: Think this thing will work?? [Re: BPJohnny]
#5469694 - 04/02/06 12:34 AM (17 years, 9 months ago) |
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If your planning on dropping ~$100 on just the greenhouse and who knows how much more to modify it as a clean room, then maybe you should consider a flow-hood. Simply a large HEPA that you work in front of. ULTRAGUARD HEPA FILTER FAN POWERED 110V
Just an idea, and if that is too pricey for you then just buy a suitable sized HEPA and build your own box and add a blower fan. It will be a little more work building your own, but its worth it in the end!
-------------------- Row, row, row your boat, Gently down the stream. Merrily, merrily, merrily, merrily, Life is but a dream.
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BPJohnny
Stranger


Registered: 03/28/06
Posts: 389
Last seen: 17 years, 7 months
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Re: Think this thing will work?? [Re: ShroomInduced]
#5493724 - 04/08/06 04:51 PM (17 years, 9 months ago) |
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OK so I've ordered everything I need. I was wondering if I could pull
air out instead of pushing air in? like putting a hole at the bottom
and hooking up a vacuum to suck bad air out and pull the good air in
through the hepa filter. It sounds the same to me but I am wondering if
i am overlooking something?
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