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Offlinedon_Genaro
Stranger
Male

Registered: 02/23/06
Posts: 30
Loc: Brisbane, AUS
Last seen: 17 years, 9 months
Pls Help. Im Contaminated.
    #5466090 - 04/01/06 01:00 AM (17 years, 9 months ago)

hey all

im attempting my first grow and i cant seem to get going!! ive tried innoculating 3 times, and everytime the jars have gotten contaminated. im working as clean as i can, trying different methods, but to no avail.
heres a litle background..

being my first time i figured i'd better start out at the bottom and work my way up. so im trying the basic PF Tek. using brf, verm and water in 250 ml jars.
im basically following the "PF Tek for simple minds" guide (http://www.fungifun.org/English/Pftek), but also borrowed extra bits and pieces form other teks. in this guide it has its measurements in ml, but since i didnt have a mearuing jug, i followed the http://www.shroomery.org/index.php/par/3372 guide and measured brf/verm mix in cups. "for each 1/2 pint jar mix: 2/3 cup verm and 1/4 cup brf and 1/4 cup water "
i used brf i purchased at an organic store, ive even tried it seperate times using flour from 2 different stores. i used distilled/purified water i bought from the supermarket. and quality vermiculite.
once i mixed it up and put it in jars with the 1 cm layer of verm ontop.
i then punched holes in the top of the jar in 4 places using the tip of a sharp knife turning it slowly until it punctured it and kept turning until it carved away a nice little hole.
covering each of these with micro-pore tape and then with alfoil i PC'ed them each for an hour and left to cool over night.
im making my own spore syringes also, since it impossible to get them over here in australia.
ive made a glove box.




before anything goes into it, i wipe it down with alcohol. i serilise the little dish which i use to make the solution in a pot of boiling water for about 20 mins and then drop into the glovebox. i fill the syringe with boiling water and flame sterilise the needle tip and then wipe with alcohol., then drop into the glove box. i make the syringe completely in here so there shouold be no chance of contam.
once done i cap the needle and take it out of the glovebox, and strap to my aquarium air pump over night for the spores to re-hydrate.
when its ready, i flame sterilise the needle, and wipe with alcohol before i innoc into the jars. punching holes through the tape and then putting a little peice of tape back over the hole.
then i leave the jars in a makeshift incubator i made like in the PF-Tek for simple minds.

this all seems right to me.. i cant figure out whats going wrong.
although there are a few little thigs...

1)ive never used a PC before and the one i got off a friend is second hand, so didnt come with any instuctions and it doesnt have a pressure guage on it. i got some instructions off here on how to use one, so i think its pretty right. with the



2)when i have washed the lids of the jars, the holes have developed a little bit of rust around the inside. when i go to innoc and the needle touches this when i poke through the hole, can this cause contam?

3)is it possible that the spore prints them selves are contam? he sent them wrapped well in foil and i have kept them that way ever since unless in the glovebox.

4)is the mixture in the right proportions that i used? "for each 1/2 pint jar mix: 2/3 cup verm and 1/4 cup brf and 1/4 cup water " cause it just seems sometimes that there is maybe too much water. it looks right before i PC, but when i take them out i notice droplets of water inside? and the amount of stuff inside almost shrinks a little.


arrgggg
its really pissing me off
here is one jar which is the worst contaminated.
i had a jar which was also yellow, but the ones this time have been purple/dark red.







any and all help is muchly apprecitaed

thanks guys :P


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OfflineMLBjammer
Invitro Freak
Male User Gallery

Registered: 02/24/06
Posts: 560
Loc: Southeastern USA
Last seen: 7 years, 14 days
Re: Pls Help. Im Contaminated. [Re: don_Genaro]
    #5466977 - 04/01/06 10:10 AM (17 years, 9 months ago)

Did you wipe down the sides of the jar before you put your dry layer of vermiculite in (sorry my question mark and comma do not work)--and you should ground your own brown rice flour from long grain brown rice with a coffee grinder--it is chunkier and feeds them better.  If your vermiculite is too coarse that could also be a problem.  I have not yet made my own syringe but you seem to be pretty careful--I would order another print just to be safe.  PF revised his BRF cake mix--1/2 cup vermiculite 1/4 cup BRF 1/4 cup water.  And look at some teks that just use prints to grow shrooms--since you already have a glovebox.  I am sure some others will give you some input.  But don't give up.  LOL. :mushroom2:


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Offlinedon_Genaro
Stranger
Male

Registered: 02/23/06
Posts: 30
Loc: Brisbane, AUS
Last seen: 17 years, 9 months
Re: Pls Help. Im Contaminated. [Re: MLBjammer]
    #5473837 - 04/03/06 08:33 AM (17 years, 9 months ago)

yeh i wiped down the edges as best i could.

if i think back to when i actually recieved the print, i think i opened it to have a look, not considering the contam factor. any air borne particles which landed on it could have contributed to the contam?

i think ill try and get another print.

cheers


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Offlinesudont
Stranger ThanFiction

Registered: 01/19/06
Posts: 129
Loc: Chicago
Last seen: 13 years, 11 months
Re: Pls Help. Im Contaminated. [Re: don_Genaro]
    #5477646 - 04/04/06 12:52 AM (17 years, 9 months ago)

First of all, why can't you order spore syringes from, say, the US? Surely there's a supplier who would ship to you?
But beyond that, working with spore prints is very difficult, and yes, the print could be contaminated. It's also possible you're going wrong with the pressure cooker. I would get one with a gauge. You have to count your time after you hit the correct pressure, (1kg per cm3), so it ends up being a lot longer than an hour total.
Another suggestion might be to use bleach rather than alcohol in your glove box. It's more effective and it's safer.
When you say you, "fill the syringe with boiling water", what is that for? Is that to sterilize the syringe, or is this water you'll work with? Either way, I would recommend pressure-cooking your dish, along with any water you'll use, and start with a new, sealed, sterile syringe. Boiling isn't 100% reliable.
As far as mixing your ingredients, that shouldn't have any effect, contam-wise. The important things are having a sterile syringe, and a sterile jar and substrate with a dry verm barrier. IMO, if you can order a spore syringe and get a decent pressure-cooker, you should have no problems. I've never had a single contaminated jar since using spore syringes. Good luck, mate!


--------------------
sudon't

sudo rm -R /*


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Offlinepercival
Stranger
Registered: 04/20/01
Posts: 1
Loc: Santa Rosa, CA USA
Last seen: 17 years, 4 months
Re: Pls Help. Im Contaminated. [Re: sudont]
    #5547527 - 04/23/06 02:01 PM (17 years, 9 months ago)

Ack, I just ended up with this exaact same purple discoloration in all of the jars of my latest batch! They look just like yours pictured above.

Is this definitely contamination or something else?
I can't tell yet, every other time I've had any type of contamination, I opened up the jar and the smell was obviously foul, these don't smell at all...is this familiar to anyone?


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