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vegitative
Stranger
Registered: 01/21/06
Posts: 97
Last seen: 17 years, 7 months
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Holes in a PF Jar
#5465116 - 03/31/06 06:43 PM (17 years, 9 months ago) |
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I was thinking, why should there be four holes? Is there some advantage over just having one big hole in the center? And wouldn't one hole help minimize the risk of a contamination since the syringe A: only goes in once, and B: there's only one hole open to the outdoors anyways?
Any ideas? I'm making a new set of jars tommorow (this is my third try at jars... my last two all got contaminated with blue/green mold) and i'm hoping to find a way that'll minimize risks.
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Snaggletooth
Stranger in a Strange Land


Registered: 10/24/05
Posts: 6,109
Loc: blinks stupidly
Last seen: 6 years, 8 months
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Re: Holes in a PF Jar [Re: vegitative]
#5465122 - 03/31/06 06:48 PM (17 years, 9 months ago) |
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For PF you Do want 4 holes
More inoculations points helps for colonization, fewer = slower colonization
You can use this so the jars can breath
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Atheist Chat
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happyhead
Stranger

Registered: 01/26/06
Posts: 44
Last seen: 16 years, 10 months
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Re: Holes in a PF Jar [Re: vegitative]
#5465124 - 03/31/06 06:49 PM (17 years, 9 months ago) |
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more holes more places to shot the needle down the side of the glass needle needs to be on the glass
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stelthvue
is frightened ofpoo
Registered: 01/20/06
Posts: 272
Last seen: 17 years, 9 months
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Re: Holes in a PF Jar [Re: happyhead]
#5465136 - 03/31/06 06:54 PM (17 years, 9 months ago) |
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i use a single hole in all my jars. this way when i see growth on the outside of the jars, i know the whole inside has growth already.
-------------------- No statements made in any post or message by myself should be construed to mean that I am now, or have ever been, participating in or considering participation in any activities in violation of any local, state, or federal laws. All posts are works of fiction.
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ShroomInduced
I read too manymycology books


Registered: 04/19/05
Posts: 309
Loc: A giant mushroom in my he...
Last seen: 12 years, 5 months
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Re: Holes in a PF Jar [Re: stelthvue]
#5465155 - 03/31/06 06:59 PM (17 years, 9 months ago) |
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the needle doesnt need to be on the glass. I put one hole in the center of my jar lids and then just point it at the sides in 4 places. as long as your not using those little ass half pint jelly jars it works.
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Hippie3
mycotopiate


Registered: 11/06/99
Posts: 3,090
Loc: mycotopia.net
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your intent is to completely cover the outside of the cake with mycellia as fast as possible that helps shut out any contams. so growth from 4 equidistant points converge to cover the surface faster than one central injection. on larger diameter cakes i use 6 holes, even faster.
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