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FractalXplora
Grainiack



Registered: 02/11/06
Posts: 2,494
Loc: UK
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a bit of advice please
#5462578 - 03/31/06 02:18 AM (17 years, 9 months ago) |
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looking to start messing with petri dishs agar. will this do the trick description from item on ebay ok....
Laboratory grade refined Potato Dextrose agar. 82 grams. Suspend at 41 gms/litre distilled water.
"If you have ever spent hours boiling and straining potatos this is like a dream come true!!
Potato extract is a mixture of potato proteins, manufactured by controlled enzymatic hydrolysis. The extract is an excellent nitrogen source for Fusarium species and many other fungi, yeasts and prokaryotes and contains vitamins and minerals as well.
In order to inhibit bacterial growth the medium may be acidified to pH 3.5 by adding tartaric acid, lactic acid or some other organic acid. The medium must not be heated after the addition of the acid, this will result in the hydrolysis of the agar and destroy its gelling properties."
Physical chemical characteristics
Solubility in water at 1 %
Complete PH (1 % solution) 6.7-7.3 Loss on drying < 6% Total nitrogen TN 9.5 -10.5 ?-amino nitrogen AN 5.0 - 5.8 % AN/TN x 100 51 - 57 Residue on ignition < 25.0 % Chloride (as NaCl) < 20.0 %
whats this a bout changing the pH of it??? i though u just steralized, cool it, pour it,and then knock em up? I wanna try cloning a nice fat GT on a dish. Is clonning really worth it, better yields? cheers <>
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MindsEye
AMT Fiend


Registered: 02/20/06
Posts: 645
Loc: cerebral cortex
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I wouldnt trust any cultivation items on ebay such as spores or agar, tools are a different story. There was a dude on here not too long ago talking about how he couldnt get ebay agar to grow anything. Pre made stuff is cheap check out the sponsors allmost all of them sell it.
-------------------- Easy Poo Cake Tek
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FractalXplora
Grainiack



Registered: 02/11/06
Posts: 2,494
Loc: UK
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Re: a bit of advice please [Re: MindsEye]
#5462818 - 03/31/06 05:35 AM (17 years, 9 months ago) |
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k m8,,,cheers
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hyphae
born to grow


Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
Last seen: 12 years, 8 months
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PDA is the shit alright also don't worry about the pH. I've bought prepoured/sterilized petri's from e-bay and they work just fine.
-------------------- Getting the most out of your casings!, A pinning strategy. Oyster Shell "Flour" $2 for 1lb. a hell of a deal Not what is overlay but rather what overlay is Gas Exchange vs. FAE "We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"
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FractalXplora
Grainiack



Registered: 02/11/06
Posts: 2,494
Loc: UK
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Re: a bit of advice please [Re: hyphae]
#5462950 - 03/31/06 07:20 AM (17 years, 9 months ago) |
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ok, im having trouble finding it from another source.......ta
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