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cokaznrebel
BBQ CHK CHZ STK
Registered: 11/08/04
Posts: 328
Loc: Mile High
Last seen: 3 years, 4 months
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Creating a LC with a spore syringe...
#5460735 - 03/30/06 05:11 PM (17 years, 9 months ago) |
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Would it be theoreticlly possible to take a vendor bought or self-made spore syringe, empty 1 CC of spore solution and suck up 1CC sterilized karo solution to create a mini-lc culture inside of the spore syringe?
Just a stoner thought....
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agar
old hand


Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: Creating a LC with a spore syringe... [Re: cokaznrebel]
#5460760 - 03/30/06 05:15 PM (17 years, 9 months ago) |
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It would be better to do it with a 60 ml syringe.
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cokaznrebel
BBQ CHK CHZ STK
Registered: 11/08/04
Posts: 328
Loc: Mile High
Last seen: 3 years, 4 months
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Re: Creating a LC with a spore syringe... [Re: agar]
#5460770 - 03/30/06 05:17 PM (17 years, 9 months ago) |
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So its possible?
I know it would be better with an 60ml syringe but I think 10CC of LC is better than 10CC of spores.
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stelthvue
is frightened ofpoo
Registered: 01/20/06
Posts: 272
Last seen: 17 years, 9 months
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Re: Creating a LC with a spore syringe... [Re: cokaznrebel]
#5460816 - 03/30/06 05:25 PM (17 years, 9 months ago) |
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thats a big waste of 9CC of spores. id understand using 1 or 2 CC though.
-------------------- No statements made in any post or message by myself should be construed to mean that I am now, or have ever been, participating in or considering participation in any activities in violation of any local, state, or federal laws. All posts are works of fiction.
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Mezcal
Registered: 08/11/05
Posts: 1,980
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Re: Creating a LC with a spore syringe... [Re: stelthvue]
#5461298 - 03/30/06 07:23 PM (17 years, 9 months ago) |
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I could see it being workable without even having any visible solution left... Why not just fill up the "empty" syringe with the solution. No doubt there will still be a few thousand viable spores on the sides and in microscopic droplets of water.
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trauma47645
The MushroomKing


Registered: 02/09/06
Posts: 771
Loc: Somewhere in this place c...
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Re: Creating a LC with a spore syringe... [Re: Mezcal]
#5461318 - 03/30/06 07:27 PM (17 years, 9 months ago) |
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Quote:
snyder said: I could see it being workable without even having any visible solution left... Why not just fill up the "empty" syringe with the solution. No doubt there will still be a few thousand viable spores on the sides and in microscopic droplets of water.
that might actually be a great idea on how to stretch your syringe to the fullest.. I will try it on my next grow and let you all know how it works
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stelthvue
is frightened ofpoo
Registered: 01/20/06
Posts: 272
Last seen: 17 years, 9 months
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Re: Creating a LC with a spore syringe... [Re: trauma47645]
#5461436 - 03/30/06 07:56 PM (17 years, 9 months ago) |
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a fine idea indeed
-------------------- No statements made in any post or message by myself should be construed to mean that I am now, or have ever been, participating in or considering participation in any activities in violation of any local, state, or federal laws. All posts are works of fiction.
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bongtoke
Mushies


Registered: 11/14/05
Posts: 1,393
Last seen: 8 years, 10 months
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Re: Creating a LC with a spore syringe... [Re: stelthvue]
#5461487 - 03/30/06 08:09 PM (17 years, 9 months ago) |
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It works great..i once had 12 jars and 1 syringe of pink buffalo..and i used all 12 cc's for 8 jars and had 4 left..so i boiled water let it cool and sucked it up and it worked jus tas good as when there was spore solution in the syringe...
-------------------- GOTTA LOVE POO CAKES!!!
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trauma47645
The MushroomKing


Registered: 02/09/06
Posts: 771
Loc: Somewhere in this place c...
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Re: Creating a LC with a spore syringe... [Re: bongtoke]
#5468249 - 04/01/06 05:09 PM (17 years, 9 months ago) |
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I actually managed to get what ever spores were left in the syringe from 3 days ago to germinate in the syringe when i filled it karo solution. There are actually little fluffy white balls of mycelium floating around.. In another week or so i am going to start innoculating so i will post again with the colonization results from this attempt
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skeletor
the dude
Registered: 09/06/05
Posts: 1,235
Loc: heaven on earth
Last seen: 17 years, 4 months
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Re: Creating a LC with a spore syringe... [Re: trauma47645]
#5468362 - 04/01/06 05:57 PM (17 years, 9 months ago) |
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you might wanna suck up some sterilized air too like from the inside of a flame. its nice and hot but beucase its air won't heat up your lc and kill your solution.
-------------------- im sorry about your mother. She was a terrible attrative woman. Get back to nature; hunting burgers and gathering fries.
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trauma47645
The MushroomKing


Registered: 02/09/06
Posts: 771
Loc: Somewhere in this place c...
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Re: Creating a LC with a spore syringe... [Re: skeletor]
#5470535 - 04/02/06 11:14 AM (17 years, 9 months ago) |
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Already did it skeletor.. I also have actually have turned a walk in closet in my house into a psudo clean room.. Positive pressure / hepa filters / and an upward laminar airflow setup.. That is where i do all my work
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