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InvisibleCaptainKirk

Registered: 07/15/05
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Re: A foolproof method for making Liquid culture [Re: _OttO_] * 1
    #5260301 - 02/03/06 06:47 PM (17 years, 11 months ago)

i just made a couple of pan cyan LC's night before last and they are ripping..like you Otto i just scraped my spores in to the lc,which is vanilla extract and a splash of karo :smirk:



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Offline_OttO_
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Re: A foolproof method for making Liquid culture [Re: CaptainKirk] * 1
    #5260360 - 02/03/06 07:05 PM (17 years, 11 months ago)

That looks really healthy mate. I love the fact you dont need to hassle about with making spore syringes and makes it easy to keep growing from your own prints.


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OfflineSparknutz420
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Re: A *beginner friendly* method for making Liquid Culture [Re: _OttO_] * 1
    #5264522 - 02/04/06 10:25 PM (17 years, 11 months ago)

yea good shit man im boiling some water right now hope it works


--------------------
Everythings changing to colors of green...


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Invisiblesever
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Registered: 02/02/03
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Re: A *beginner friendly* method for making Liquid Culture *DELETED* *DELETED* [Re: Sparknutz420] * 1
    #5267609 - 02/05/06 09:28 PM (17 years, 11 months ago)

~


Edited by sever (07/17/06 02:43 PM)


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Offline_OttO_
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Re: A *beginner friendly* method for making Liquid Culture [Re: sever] * 1
    #5268314 - 02/06/06 01:14 AM (17 years, 11 months ago)

Probably best to start a new thread for something like this, and without being able to say exactly what went wrong - I can say that you needed to sterilise the jar again after the honey was added.

The smell *might* have been a sign of bacterial contamination.


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Invisiblesever
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Re: A *beginner friendly* method for making Liquid Culture *DELETED* [Re: _OttO_] * 1
    #5268754 - 02/06/06 08:20 AM (17 years, 11 months ago)

~


Edited by sever (07/17/06 02:43 PM)


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OfflineBeruthel
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Re: A foolproof method for making Liquid culture [Re: sever] * 1
    #5287590 - 02/10/06 11:09 PM (17 years, 11 months ago)

This method looks like a wonderful first time, and I shall soon discover so. Thanks for this wonderful guide, saved me.


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Invisiblegiz
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Re: A foolproof method for making Liquid culture [Re: _OttO_] * 1
    #5287704 - 02/11/06 12:23 AM (17 years, 11 months ago)

Thank you for writing this (5 shrooms for you) , this tek was just what I needet :smile:


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Offlinecougercruiser
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Re: A foolproof method for making Liquid culture [Re: giz] * 1
    #5287809 - 02/11/06 01:32 AM (17 years, 11 months ago)

ohh key key.....meow~


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OfflineApache
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Re: A foolproof method for making Liquid culture [Re: _OttO_] * 1
    #5292423 - 02/12/06 07:10 PM (17 years, 11 months ago)

Thank you so much! I am well on my way to may first try...


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Offlinediablovt1125
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Re: A foolproof method for making Liquid culture [Re: Apache] * 1
    #5341267 - 02/26/06 02:38 PM (17 years, 10 months ago)

I'm trying my hand at this whole LC thing after reading this simple TEK, good work! I'll let you all know how it goes, just did them up this morning with the last cc or two of a South American Syringe I've had lying around.


Check out my cool jars!



--------------------
Know yourself, know your substance, and know your source


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OfflineCorteX_
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Re: A foolproof method for making Liquid culture [Re: diablovt1125] * 1
    #5341899 - 02/26/06 05:17 PM (17 years, 10 months ago)

This is really a great tek.. I'm gonna try this ASAP!
5 shrooms for you my man!

peace


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OfflineShimen
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Re: A foolproof method for making Liquid culture [Re: CorteX_] * 1
    #5342438 - 02/26/06 07:24 PM (17 years, 10 months ago)

I have tried this method and it seems after 3 days that i have myc growth. Kinda transparent cotteny looking shit sitting at the bottom of my jar. Cool Tek thanks.


--------------------
Death is when you become bored with life.


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InvisibleliloldmeFacebook
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Re: A foolproof method for making Liquid culture [Re: Shimen] * 1
    #5344034 - 02/27/06 02:25 AM (17 years, 10 months ago)

I like this, I'm giving it a shot right now...


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InvisibleFractalXplora
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Re: A *beginner friendly* method for making Liquid Culture [Re: _OttO_] * 1
    #5344233 - 02/27/06 05:08 AM (17 years, 10 months ago)

nice tek i will try my last LC did nothing :smile:


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Offline_OttO_
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Re: A *beginner friendly* method for making Liquid Culture [Re: FractalXplora] * 1
    #5344592 - 02/27/06 09:22 AM (17 years, 10 months ago)

Good luck and make sure you post your results!


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OfflineAloysius
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Re: A *beginner friendly* method for making Liquid Culture [Re: _OttO_] * 1
    #5450388 - 03/28/06 08:59 AM (17 years, 9 months ago)

This might be worlds stupidest question, but if I took some tissue from the inside of a shrooms' stem, and tossed that in a pot of water/honey solution (everything as sterile as possible of course)..

Would I see some growth?


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OfflineBamaman
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Re: A *beginner friendly* method for making Liquid Culture [Re: Aloysius] * 1
    #5451504 - 03/28/06 02:26 PM (17 years, 9 months ago)

Question:

I saw it said that syringes of your lc will keep in the fridge for months. What about leaving it in the jar, baby bottle, etc...? I would assume that it would "keep" in the original LC jar as well, right?

It would be alot less conspicuous for long term storage in your fridge.


--------------------
Diabetes causes hamsters.


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Offlineendlesswinter77
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Re: A foolproof method for making Liquid culture [Re: Bamaman] * 1
    #5451617 - 03/28/06 02:54 PM (17 years, 9 months ago)

nice way to lay it out. That is basically the way I did my first LC, I skipped the initial sterilization of the jar and just did it when the liquid was put in... worked fine.


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There's some things a man has to prove to himself alone... not to anyone else.


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Offline_OttO_
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Re: A *beginner friendly* method for making Liquid Culture [Re: Aloysius] * 1
    #5454136 - 03/29/06 07:56 AM (17 years, 9 months ago)

Quote:

Aloysius said:
This might be worlds stupidest question, but if I took some tissue from the inside of a shrooms' stem, and tossed that in a pot of water/honey solution (everything as sterile as possible of course)..

Would I see some growth?




Yep, sure would! You would be 'cloning' the mushroom the peice of stem came from. :thumbup:

Quote:

Bamaman said:
Question:

I saw it said that syringes of your lc will keep in the fridge for months. What about leaving it in the jar, baby bottle, etc...? I would assume that it would "keep" in the original LC jar as well, right?

It would be alot less conspicuous for long term storage in your fridge.




Definately - I dont remember saying to suck it up and store, I just keep the jars of mycelium in the fridge - and suck some up as you need to use it.

This is still working fine for me and still hasnt contammed yet. I have been having casing contams lately - I have been a bit lasy and too busy - but its nothing to do with the LC and I still have never had one jar contaminate.

I always open air inoculate the LC by just scraping up some spores with a knife tip and dropping them into the jar.


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