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OfflineBPJohnny
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Registered: 03/28/06
Posts: 389
Last seen: 17 years, 7 months
Sterile Lab
    #5453029 - 03/28/06 08:56 PM (17 years, 10 months ago)

Hi, I am wondering if anyone can share any plans or current setups for a sterile or semi-sterile enviroment. please share any tips hints or things that do not work from personal experience, Thanks!

--BPJohnny--


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Offlinestelthvue
is frightened ofpoo
Registered: 01/20/06
Posts: 272
Last seen: 17 years, 9 months
Re: Sterile Lab [Re: BPJohnny]
    #5453071 - 03/28/06 09:06 PM (17 years, 10 months ago)

I turn on the fan in my bathroom hours before using it and spray oust a few times. I keep the door closed and wiped down my counter with alcohol.


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No statements made in any post or message by myself should be construed to mean that I am now, or have ever been, participating in or considering participation in any activities in violation of any local, state, or federal laws. All posts are works of fiction.


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Invisiblemusher_420
Stranger

Registered: 08/01/05
Posts: 2,691
Re: Sterile Lab [Re: BPJohnny]
    #5453080 - 03/28/06 09:08 PM (17 years, 10 months ago)

Well for simplicity a still air glove box works great as a small sterile environment when working with G2G transfers or spore prints/syringes that sort of thing.

Something easier to work with and probably more reliable but harder to make and more expensive would be a hepa filtered flow hood.

Unless your talking about creating a positive pressure clean room or something. The only guy I have heard talk about one of those was Agar.


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My Humidifier Reservoir Expansion Tek

Must read links for beginners

Btw- You are not what you own!


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OfflineBPJohnny
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Registered: 03/28/06
Posts: 389
Last seen: 17 years, 7 months
Re: Sterile Lab [Re: stelthvue]
    #5453090 - 03/28/06 09:08 PM (17 years, 10 months ago)

I am looking for something a little more permanent something i can install a hepa filter in and keep my petri dishes in there without fear of contamination


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InvisibleRoadkillM
Retired Shroomery Mod
Male User Gallery

Registered: 12/11/01
Posts: 22,674
Loc: Montana
Trusted Cultivator
Re: Sterile Lab [Re: BPJohnny]
    #5453123 - 03/28/06 09:16 PM (17 years, 10 months ago)

do you have a spare room?

if you do...I'll share some thoughts.

if not...build a still air box.


tc


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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OfflineBPJohnny
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Registered: 03/28/06
Posts: 389
Last seen: 17 years, 7 months
Re: Sterile Lab [Re: Roadkill]
    #5453128 - 03/28/06 09:17 PM (17 years, 10 months ago)

Yes i do have a spare room, a room that i want to dedicate


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InvisibleRoadkillM
Retired Shroomery Mod
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Registered: 12/11/01
Posts: 22,674
Loc: Montana
Trusted Cultivator
Re: Sterile Lab [Re: BPJohnny]
    #5453201 - 03/28/06 09:36 PM (17 years, 10 months ago)

make a clean room if you have an extra room.


pull everything out of the room and clean it really good.

go to Home Depot or some kind of home improvement type hardware store.

buy a roll of Visqueen(Heavy Duty Clear Plastic)...the thicker the better.

Close off any vents to the room...if you need heat put filters inside the vents.

Cover the walls, Ceiling and floor with the Visqueen.

You can use duct tape to hold the visqueen in place...or you can screw wooden slats of wood in the corners to hold the visqueen in place.

I'd make clean room curtains to enter the room.

then the sky is the limit with what you put into it.


tc


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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OfflineBPJohnny
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Male

Registered: 03/28/06
Posts: 389
Last seen: 17 years, 7 months
Re: Sterile Lab [Re: Roadkill]
    #5453217 - 03/28/06 09:40 PM (17 years, 10 months ago)

exactly what i had in mind thats excellent. for air exchange would it be sufficient to cut holes in the plastic and seal the hole with filters?


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InvisibleTippinthru
contented

Registered: 04/07/05
Posts: 1,131
Loc: "The Garden"...
Re: Sterile Lab [Re: Roadkill]
    #5453466 - 03/28/06 10:46 PM (17 years, 10 months ago)

Quote:

Roadkill said:
make a clean room if you have an extra room.


pull everything out of the room and clean it really good.

go to Home Depot or some kind of home improvement type hardware store.

buy a roll of Visqueen(Heavy Duty Clear Plastic)...the thicker the better.

Close off any vents to the room...if you need heat put filters inside the vents.

Cover the walls, Ceiling and floor with the Visqueen.

You can use duct tape to hold the visqueen in place...or you can screw wooden slats of wood in the corners to hold the visqueen in place.

I'd make clean room curtains to enter the room.

then the sky is the limit with what you put into it.


tc





Great Advice! I was going to answer your post however,
RK says it well! Boy aren't you on a roll tonight!  :congrats:


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Perfection is attained by slow degrees; it requires the hand of time...
[


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