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Ettin
Stranger

Registered: 03/28/06
Posts: 33
Last seen: 17 years, 9 months
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My dog on agar
#5451942 - 03/28/06 04:35 PM (17 years, 10 months ago) |
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This is kind of a weird topic, but for those with experience with agar...
My dog has been having skin problems, so i took him into the vet to get some tests done, one was a fungal test. the vet took a skin scraping and stuck it in a vial of agar. He just opened the vial up and stuck it right in. Now i havent had any experience working with agar, but i thought that inorder to get a pure sample of whatever it is your trying to colonize, that the sample must be added in a clean room, because all the spores floating around in the air will fly onto the agar and colonize along your sample.
So was what the vet did legit? can i rest assured that whatever colonizes on the agar will be from my dog and not some airborne spore?
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Snaggletooth
Stranger in a Strange Land


Registered: 10/24/05
Posts: 6,109
Loc: blinks stupidly
Last seen: 6 years, 8 months
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Re: My dog on agar [Re: Ettin]
#5451968 - 03/28/06 04:41 PM (17 years, 10 months ago) |
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Yeah, that's "field" work.
I had near the same thing with my bunny.
When she busted it out, I was like I know what that is.
And we talked for a few about it, and then she let me in back to take a look and a skin sample under the microscope.
And a god way to learn a little more next time start up a conversation. My vet was surprised that I new what I did..
--------------------
Atheist Chat
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endlesswinter77
Nomadic Herdsman


Registered: 11/09/05
Posts: 106
Loc: Nor Wes
Last seen: 12 years, 1 month
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I have very small experience with this, but I remember doing an experiment a while back with a veterinarian where we took samples of peoples skin and grew bacteria on sheeps blood in petri dishes. Certain colored growths signified different bacteria. So with the dogs skin sample, the air might leave different spores in it, but the vet probably knows what to look for in a variety of growths to signify a fungus problem on your dogs skin.
-------------------- There's some things a man has to prove to himself alone... not to anyone else.
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Ettin
Stranger

Registered: 03/28/06
Posts: 33
Last seen: 17 years, 9 months
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oh, i guess ill take his word for it then.
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Rahz
Alive Again


Registered: 11/10/05
Posts: 9,230
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Re: My dog on agar [Re: Ettin]
#5452319 - 03/28/06 06:08 PM (17 years, 10 months ago) |
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I'd say there's so much on a dog (especially if there's an infection) that a few seconds of air exposure wouldn't affect the results. Anything floating around in the air is likely to be found on the dog anyway.
Rahz
-------------------- rahz comfort pleasure power love truth awareness peace "You’re not looking close enough if you can only see yourself in people who look like you." —Ayishat Akanbi
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starseed1066
officially hosed

Registered: 04/13/05
Posts: 325
Loc: close enough to get mysel...
Last seen: 11 years, 7 months
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Re: My dog on agar [Re: Rahz]
#5452374 - 03/28/06 06:20 PM (17 years, 10 months ago) |
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Quote:
Rahz said: I'd say there's so much on a dog (especially if there's an infection) that a few seconds of air exposure wouldn't affect the results. Anything floating around in the air is likely to be found on the dog anyway.
Rahz
lol! and yes, you're probably right...
-------------------- Under his instruction, I taped drumsticks to his head and turned him into a sort of mummy, or perhaps a caterpiller in a cocoon. He remains this way for about three hours, making bizarre noises, pretending to be a new species that must learn to walk and talk and eat, etc. And he communicates with other life forms by way of the antennae on his head. Eventually we jam a tube from a waterbong into it's mouth and figure he's learned all he needs to know.
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mogur
regnartS

Registered: 11/15/05
Posts: 322
Loc: Puget Sound
Last seen: 11 years, 2 months
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And here I was thinking someone was dogging on my man, agar. Glad to hear it's only a dog gone skin problem.
Not to belittle your dog's problem, there, Ettin. If the sample did get contam'd, it would only be a false positive, which would show up in subsequent tests.
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