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Offlinestelthvue
is frightened ofpoo
Registered: 01/20/06
Posts: 272
Last seen: 17 years, 9 months
Spores directly to LC
    #5449725 - 03/28/06 12:28 AM (17 years, 10 months ago)

Has anyone ever attempted rigging a LC up so that a fresh mushroom that hasnt dropped spores yet drops spores into the LC? Besides sterility, what else could put a damper on this idea?


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No statements made in any post or message by myself should be construed to mean that I am now, or have ever been, participating in or considering participation in any activities in violation of any local, state, or federal laws. All posts are works of fiction.


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OfflineSolidcell
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Re: Spores directly to LC [Re: stelthvue]
    #5449757 - 03/28/06 12:58 AM (17 years, 10 months ago)

You mean positioning the jar under the cap so the spores drop straight into the solution?

Are you trying to build a Rube Goldberg Machine? Try setting up some dominoes to hit a bowling ball to roll and knock the jar into place. That'd be sweet.

In all seriousness, was my first assumption correct?


--------------------
Psychedelic Jar Project

"It is dangerous to be right when the government is wrong." -Voltaire

"Everything that I accepted as being true up to now I acquired from the senses or through the senses. However, I have occasionally found that they deceive me, and it is prudent never to trust those who have deceived us, even if only once." -Descartes


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Offlinestelthvue
is frightened ofpoo
Registered: 01/20/06
Posts: 272
Last seen: 17 years, 9 months
Re: Spores directly to LC [Re: Solidcell]
    #5449769 - 03/28/06 01:06 AM (17 years, 10 months ago)

yup. ever try it?


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No statements made in any post or message by myself should be construed to mean that I am now, or have ever been, participating in or considering participation in any activities in violation of any local, state, or federal laws. All posts are works of fiction.


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OfflineEquilibriuM
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Re: Spores directly to LC [Re: stelthvue]
    #5450216 - 03/28/06 07:47 AM (17 years, 10 months ago)

You must have a clean room. I wouldnt dare try something like that...


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Offlinefireworks_godS
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Re: Spores directly to LC [Re: EquilibriuM]
    #5450224 - 03/28/06 07:52 AM (17 years, 10 months ago)

Would it be feasible in a glovebox or something?

Theoretically, it would potentially work better in that it eliminates the extra step of making a spore print, which would be one more possible chance for contamination... I couldn't personally think of any way that one would devise a manner in which to try this; but, if anything, it couldn't hurt to try it out. :grin:

:headbang: :headbang: :headbang: :satansmoking:
Peace. :mushroom2:


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If I should die this very moment
I wouldn't fear
For I've never known completeness
Like being here
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:heartpump: :bunnyhug: :yinyang:

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Offlinecampinman
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Re: Spores directly to LC [Re: fireworks_god]
    #5450321 - 03/28/06 08:31 AM (17 years, 10 months ago)

couldnt you just carve a piece of inner stem and drop it into your LC jar as well?


i KNOW ive seen a post from agar with a little wire rig so the spores drop right into the jar. the wires keep the cap suspended above the jar. ill search for it.

im not sure why you would want an entire sporeprint in one jar, seems like overkill to me.


EDIT::: HERES THAT LINK

http://www.shroomery.org/forums/showflat...rue#Post4544725

or search for easy spore to water... by agar.


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No statements made in any post or message by myself should be construed to mean that I am now, or have ever been, participating in or considering participation in any activities in violation of any local, state, or federal laws. All posts are works of fiction.


"I wish my lawn was emo, so it would cut itself."


Edited by campinman (03/28/06 08:34 AM)


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Offlinefireworks_godS
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Re: Spores directly to LC [Re: campinman]
    #5450352 - 03/28/06 08:44 AM (17 years, 10 months ago)

Quote:

campinman said:
im not sure why you would want an entire sporeprint in one jar, seems like overkill to me.




Super fast colonization? :lol: That's all I could think of. :wink:

:headbang: :headbang: :headbang: :satansmoking:
Peace. :mushroom2:


--------------------
:redpanda:
If I should die this very moment
I wouldn't fear
For I've never known completeness
Like being here
Wrapped in the warmth of you
Loving every breath of you

:heartpump: :bunnyhug: :yinyang:

:yinyang: :levitate: :earth: :levitate: :yinyang:


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Offlinethenewguy05
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Re: Spores directly to LC [Re: fireworks_god]
    #5450376 - 03/28/06 08:55 AM (17 years, 10 months ago)

i have done something similar with spores and water but not LC.

you make a "X" that hooks onto the sides of the jar and has a basket.
make an "X" with the wire and then bend it to fit inside the jar and hook over the edges of the jar. then place it inside a jar and place a cap on the wire above the water.

i beleive this method would work only in a glove box.


Edited by thenewguy05 (03/28/06 10:04 AM)


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OfflineIGnosticAbhorI
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Re: Spores directly to LC [Re: fireworks_god]
    #5450591 - 03/28/06 09:58 AM (17 years, 10 months ago)

Quote:

fireworks_god said:
Quote:

campinman said:
im not sure why you would want an entire sporeprint in one jar, seems like overkill to me.




Super fast colonization? :lol: That's all I could think of. :wink:

:headbang: :headbang: :headbang: :satansmoking:
Peace. :mushroom2:




It won't speed colonization. Agar had a big thread on it somewhere. It doesn't hurt but it doesn't really help in a big way.

The only good thing I see about it is this :

one print > 5 LC > X syringes > X jars....

If that ONE print was contamed...Now X jars are lost =-o.

However :

one print > 1 LC > X syringes > X jars...

Atleast this way you diminish the possibilty of screwing up a bunch of jars due to ONE print...

If you had a clean room it'd be no prob.

You could try a glovebox...But think about it...

Right before the veil broke, you'd have to put the casing/cake into a glovebox, then sterilyze it...How would you do that w/o harming it? lol.

It'd be possible if you could have a small grow in a glovebox from begining to finish...

But that might be a bit more work than necessary...Unless you have a small success rate(like 5%) with LC's and jars in general.

Anyways, gl coming up with something. tc

-T


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Offlinestelthvue
is frightened ofpoo
Registered: 01/20/06
Posts: 272
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Re: Spores directly to LC [Re: IGnosticAbhorI]
    #5450709 - 03/28/06 10:30 AM (17 years, 10 months ago)

yeah im gonna try this after my first flush. ill write up a tek about it and let you guys know how everything goes.


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No statements made in any post or message by myself should be construed to mean that I am now, or have ever been, participating in or considering participation in any activities in violation of any local, state, or federal laws. All posts are works of fiction.


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Offlinestelthvue
is frightened ofpoo
Registered: 01/20/06
Posts: 272
Last seen: 17 years, 9 months
Re: Spores directly to LC [Re: stelthvue]
    #5450745 - 03/28/06 10:38 AM (17 years, 10 months ago)

wouldnt more spores per LC speed up colonization of the LC?


--------------------
No statements made in any post or message by myself should be construed to mean that I am now, or have ever been, participating in or considering participation in any activities in violation of any local, state, or federal laws. All posts are works of fiction.


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OfflineIGnosticAbhorI
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Re: Spores directly to LC [Re: stelthvue]
    #5450754 - 03/28/06 10:40 AM (17 years, 10 months ago)

Awesome.

If you got a camera, take some pics along with it.

Pics def. say a thousand words.

tc and gl

-Gnostic


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Offlinestelthvue
is frightened ofpoo
Registered: 01/20/06
Posts: 272
Last seen: 17 years, 9 months
Re: Spores directly to LC [Re: IGnosticAbhorI]
    #5450765 - 03/28/06 10:42 AM (17 years, 10 months ago)

ill definately be taking pics


--------------------
No statements made in any post or message by myself should be construed to mean that I am now, or have ever been, participating in or considering participation in any activities in violation of any local, state, or federal laws. All posts are works of fiction.


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OfflineIGnosticAbhorI
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Re: Spores directly to LC [Re: stelthvue]
    #5450782 - 03/28/06 10:47 AM (17 years, 10 months ago)

Quote:

stelthvue said:
wouldnt more spores per LC speed up colonization of the LC?




To anwser your question. No...lol

However, if you add liquid myc. to an LC jar...then the More per ml the better.

More spores just mean more spores. They still have to germinate. More of them won't speed up germination.

That's what I read from one of Agar's posts way back when. I'll look for it, in case this question comes up again and I need some physical evidence :laugh:

Tc man

-Gnostic


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Offlinestelthvue
is frightened ofpoo
Registered: 01/20/06
Posts: 272
Last seen: 17 years, 9 months
Re: Spores directly to LC [Re: IGnosticAbhorI]
    #5450790 - 03/28/06 10:50 AM (17 years, 10 months ago)

but if there are more spores to germinate, the LC gets fully germinated faster, does it not?


--------------------
No statements made in any post or message by myself should be construed to mean that I am now, or have ever been, participating in or considering participation in any activities in violation of any local, state, or federal laws. All posts are works of fiction.


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OfflineIGnosticAbhorI
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Re: Spores directly to LC [Re: stelthvue]
    #5450835 - 03/28/06 10:59 AM (17 years, 10 months ago)

I knew I'd have to look it up. Lol.

I'll let this issue rest till I find the thread.

I never could remeber how to answer that question properly. lol.

Tc man

I'll send you the link whenever I find it.

-Gnostic


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OfflineSolidcell
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Re: Spores directly to LC [Re: IGnosticAbhorI]
    #5452538 - 03/28/06 06:56 PM (17 years, 10 months ago)

I'm with stelthvue, more spores would help colonize a LC faster. Think about it, if you had one spore in there, it would germinate as fast as 1 million would, but the 1 million spores are in 1 million differnent places as opposed to the one spot the 1 spore is. It's like the concept of having 4 inoc. points in a pf jar instead of just one. The more, the better.

I'd like to see the link you mentioned. As for a thread being physical evidence, it's only someone's opinion (even though it is agar's, who I as well as MANY others follow word for word), no one has all the answers, so don't feel like you can't judge things for yourself as you see them.

As for the idea of the wire X suspended above the jar, it seems pretty cool, I must admit, but I can never see this as being more time saving, less contaminant prone, easier, or more efficient. That's just the way I see it, but I'd definatly like to see it done. Good luck stelthvue!


--------------------
Psychedelic Jar Project

"It is dangerous to be right when the government is wrong." -Voltaire

"Everything that I accepted as being true up to now I acquired from the senses or through the senses. However, I have occasionally found that they deceive me, and it is prudent never to trust those who have deceived us, even if only once." -Descartes


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