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Offlinejamman
SCI fan

Registered: 12/14/05
Posts: 280
Last seen: 16 years, 10 months
Crystal Clear LC's the easy way
    #5447083 - 03/27/06 11:25 AM (17 years, 10 months ago)

First off, this tek is really just a combination of other teks around here so credit goes to those who came before me and sorry if this has been posted before, but I couldn't find anything in a quick search.... 

The problem with honey IME is that it either caramelizes or becomes cloudy after PC'ing.  I do the following to make sure my LC's are crystal clear each time I inoculate.

In order to make LC's, one needs to have a suitable container.  There are many different ways to make LC jars, so pick whatever design you like best. I go with Agar's LC Lids that don't leak :thumbup: :thumbup:

After you've got your containers taken care of, fill each jar with water until you've reached the desired volume (I fill about 2/3 full) and empty into a medium pot on medium-low heat.  Grab your honey, karo, etc and a tablespoon or teaspoon depending on jar size.  Now comes the hardest part, mental math. 

If a 4% final solution of Honey and Water is needed, then figure that for each 100 mL you need 4mL of Honey. So for (1) Qt jar filled to    600mL, you would need 24mL of Honey. 

1 tablespoon (tbsp) = 15mL
1 teaspoon (tsp) = 5mL   

So 5 teaspoons or about2 tablespoons would make a 4% solution.
The bitch about honey is that it sticks to the measuring spoon, but if you use the spoon to stir the heated water, you'll find that it goes into solution (and off the spoon) rather easily. This makes for less of a mess and more accurate measurements than using a coffee maker.



Give that just a few minutes on the stove on low heat. While that's doing its thing, grab a couple coffee filters and place them over the mouth of your jar. Transfer the pot of honey water to a more "pour friendly" receptacle.  Filter the honey water solution through the coffee filter and prepare your jars to be sterilized (ie foil).


(karo water in this pic)

Even if only PC'ing for 15 minutes at 15 psi, it seems like my solutions caramelize, so I switched to boiling. Either in a large pot or in a PC, add the jars to some warm water and bring up to a boil (For my PC, i just put the lid on, but not the weight).  Once its boiling let it go for about half an hour, then remove from heat and let cool completely



Then either inject spores from a print/syringe, a sample from another LC or a slurry and let incubate for a week or two.



Thanks to the Shroomery, the source of all my mycology information!!!


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