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Offlinefireworks_godS
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LC! Normally This Fucking Fast?!
    #5446925 - 03/27/06 10:44 AM (17 years, 10 months ago)

Yesterday, my friend inoculated three half pint jars of lc. He filled the jars around 3/4 full with water, and added a tablespoon of Rice's Lucky Clover Honey. He shot them up with 2 cc's each of Cambodians from TLG (:thumbup:).

Anyways, he put them in the TiT incubator, with the temperature at 82 degrees. My friend opened the incubator today to add five jars of pf jars he had just inoculated. He almost didn't check the lc's because otto's tek states that you will notice some small strands of mycelium forming after a few days, and my friend is forcing himself to be patient.

Of course, otto's tek also states to shake them up once a day to encourage growth, so that meant he got to take a peek at them. :grin: Anyways, they were all crazy full of mycelium! :shocked:

Unfortunately, my friend doesn't have a digital camera right now, as his girlfriend dropped it in a river while canoeeing a year ago. However, I figure that, once the mycelium settles back down (I shook 'em up), I could measure from the bottom to somewhat convey how much mycelium is in these things.

Maybe this isn't too out of the ordinary, but it certainly wasn't what he expected, as he had the impression it would be a couple of days for the spores to germinate. Is it? :grin:

My friend wishes to thank everyone for the information he has been swimming in for the last couple of weeks, and is glad he is applying it well enough to see some sweet results! :cool:

No wonder they call it Lucky Clover honey.  :rolleyes:  :smirk:

:headbang: :headbang: :headbang: :satansmoking:
Peace. :mushroom2:


--------------------
:redpanda:
If I should die this very moment
I wouldn't fear
For I've never known completeness
Like being here
Wrapped in the warmth of you
Loving every breath of you

:heartpump: :bunnyhug: :yinyang:

:yinyang: :levitate: :earth: :levitate: :yinyang:


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InvisibleMindsEye
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Re: LC! Normally This Fucking Fast?! [Re: fireworks_god]
    #5446940 - 03/27/06 10:48 AM (17 years, 10 months ago)

Was it a vented LC container, when I used baby bottles it would take a few days for spores to germinate, but when I switched to a vented LC they germinate within 1-2 days...


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Easy Poo Cake Tek


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OfflineLouddrum
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Re: LC! Normally This Fucking Fast?! [Re: MindsEye]
    #5446990 - 03/27/06 10:59 AM (17 years, 10 months ago)

SOrry this is off topic but when injecting your casing with lc, can your casing be just poo, or does the poo have to be sandwiched by other material's(verm, coco ect.)?


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InvisibleMindsEye
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Re: LC! Normally This Fucking Fast?! [Re: Louddrum]
    #5447002 - 03/27/06 11:01 AM (17 years, 10 months ago)

Quote:

Louddrum said:
SOrry this is off topic but when injecting your casing with lc, can your casing be just poo, or does the poo have to be sandwiched by other material's(verm, coco ect.)?




I dont think anyone on here injects their casing's, they grow spawn jar usually some type of grain and spawn that to poo...


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Easy Poo Cake Tek


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Offlinefireworks_godS
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Re: LC! Normally This Fucking Fast?! [Re: MindsEye]
    #5447020 - 03/27/06 11:05 AM (17 years, 10 months ago)

Quote:

MindsEye said:
Was it a vented LC container, when I used baby bottles it would take a few days for spores to germinate, but when I switched to a vented LC they germinate within 1-2 days...




My friend had two holes in each lid and taped them over with micropore. He put a second hole in so that pressure wouldn't make it hard to suck up the mycelium when it became time to inoculate cakes with them. He inoculated through the tape and covered the hole immediately with more micropore tape soaked in alcohol, as agar suggested to do. :wink:

I wish I could take a picture! :mad: A lot of the mycelium is still in suspension, but most of it has settled back down. It completely covers the bottom of the jar and sticks up a lot further than that in a lot of spaces.

:cool:

:headbang: :headbang: :headbang: :satansmoking:
Peace. :mushroom2:


--------------------
:redpanda:
If I should die this very moment
I wouldn't fear
For I've never known completeness
Like being here
Wrapped in the warmth of you
Loving every breath of you

:heartpump: :bunnyhug: :yinyang:

:yinyang: :levitate: :earth: :levitate: :yinyang:


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InvisiblePremedman1
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Re: LC! Normally This Fucking Fast?! [Re: MindsEye]
    #5447027 - 03/27/06 11:07 AM (17 years, 10 months ago)

If it's full of growth in less than a day after MS inoculation, I doubt it's myc. Some contams can grow very quickly, and can take over a LC in a very short period of time. I hope I'm wrong, though.


--------------------
Build a man a fire, he is warm for the night. Set a man on fire, he is warm for the rest of his life.


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Offlinefireworks_godS
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Re: LC! Normally This Fucking Fast?! [Re: Premedman1]
    #5447042 - 03/27/06 11:11 AM (17 years, 10 months ago)

Well, its been a little over a day. :grin: "MS" = multi-spore?

My friend is concerned that it is contamination. It is white, though. :confused:

I'll start searching through pictures of lc to see if I can discern a difference... is there eventually any way to tell? Or would I have to suck it up and stick it into a cake, and then let it grow out to find out?

:headbang: :headbang: :headbang: :satansmoking:
Peace. :mushroom2:


--------------------
:redpanda:
If I should die this very moment
I wouldn't fear
For I've never known completeness
Like being here
Wrapped in the warmth of you
Loving every breath of you

:heartpump: :bunnyhug: :yinyang:

:yinyang: :levitate: :earth: :levitate: :yinyang:


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Offlinefireworks_godS
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Re: LC! Normally This Fucking Fast?! [Re: fireworks_god]
    #5447170 - 03/27/06 11:57 AM (17 years, 10 months ago)

So far, I'm not too inclined to think that my friend has contamination. For one thing, it looks almost exactly like the picture in otto's tek. Around the edges where the stuff settles together, it is white, and it gets yellow/golden in the clumps (from the honey, of course). :grin:

The pieces that float around are all white, and some of them are more strands than anything. If the pieces aren't already floating, one simple movement propels them. The threads I read, searching for "liquid culture mycelium", support this, and state that it would be like tiny pieces of tissue paper, or something.

One thread I read, where the guy was in the same situation (one day, no camera :lol:), was that, if the syringe was chock-full of spores, it isn't surprising to see that much growth that quickly, and TLG syringes always kick ass, in my experience.

Anyways, he shook them up again and put them back in the incubator, and is looking forward to seeing them tomorrow morning. He will probably suck some up and shoot it into a pf jar tomorrow out of pure curiousity to find out! :grin:

:headbang: :headbang: :headbang: :satansmoking:
Peace. :mushroom2:


--------------------
:redpanda:
If I should die this very moment
I wouldn't fear
For I've never known completeness
Like being here
Wrapped in the warmth of you
Loving every breath of you

:heartpump: :bunnyhug: :yinyang:

:yinyang: :levitate: :earth: :levitate: :yinyang:


Edited by fireworks_god (03/27/06 11:58 AM)


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OfflineMushroommanToad
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Registered: 01/28/06
Posts: 21
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Re: LC! Normally This Fucking Fast?! [Re: fireworks_god]
    #5447218 - 03/27/06 12:10 PM (17 years, 10 months ago)

I just started some LC jars about a week ago. They were started from tissue (clone). They are fast. I will be innoculating my grain jars tommorrow. I have enough to inject as much as I need. They should colonize fast. I can't wait to see the results. I use to be an agar guy, but LC blows it out the water!


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...and she's buying a stairway to heaven.


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InvisibleMindsEye
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Re: LC! Normally This Fucking Fast?! [Re: Premedman1]
    #5447451 - 03/27/06 01:09 PM (17 years, 10 months ago)

Quote:

Premedman1 said:
If it's full of growth in less than a day after MS inoculation, I doubt it's myc. Some contams can grow very quickly, and can take over a LC in a very short period of time. I hope I'm wrong, though.




I have spores germinate in LC's all the time as long as they are vented in 24 hrs, they arent contaminated...


--------------------
Easy Poo Cake Tek


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InvisiblePremedman1
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Re: LC! Normally This Fucking Fast?! [Re: MindsEye]
    #5447618 - 03/27/06 01:39 PM (17 years, 10 months ago)

Quote:

MindsEye said:
Quote:

Premedman1 said:
If it's full of growth in less than a day after MS inoculation, I doubt it's myc. Some contams can grow very quickly, and can take over a LC in a very short period of time. I hope I'm wrong, though.




I have spores germinate in LC's all the time as long as they are vented in 24 hrs, they arent contaminated...




Listen friend, I value your opinion as much as any other member of this community. We all have new things to learn about this hobby, as I learn something new every day. But if you're going to quote me, keep what I said in context. You said you have spores GERMINATE in LC's within 24 hours, which is totally acceptable and believable, but the originator of this post said the LC was "crazy full" of mycelium within less than a day of a MS inoc, which I said was highly improbable if not impossible. Again, thanks for your insightful post, but next time please refrain from using my posts to make your point unless you fully understand my point.  :grin:


--------------------
Build a man a fire, he is warm for the night. Set a man on fire, he is warm for the rest of his life.


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InvisibleMindsEye
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Re: LC! Normally This Fucking Fast?! [Re: Premedman1]
    #5447653 - 03/27/06 01:47 PM (17 years, 10 months ago)

Well it was crazy full of spores when he injected it if all the spores germinated then you have a lot of growth in a short period, I didnt take anything out of context here, and I wasnt trying to dog on you so dont get butt hurt if somone quotes you, and that is why the quote button is there man. Peace man damn you need to lighten up a little.


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Offlinefireworks_godS
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Re: LC! Normally This Fucking Fast?! [Re: MindsEye]
    #5447822 - 03/27/06 02:43 PM (17 years, 10 months ago)

My friend found a way to take some pictures. It might be a couple of days before he can get them on the computer, but they are taken so these things are documented, and this thread will be updated then. :wink:

:headbang: :headbang: :headbang: :satansmoking:
Peace. :mushroom2:


--------------------
:redpanda:
If I should die this very moment
I wouldn't fear
For I've never known completeness
Like being here
Wrapped in the warmth of you
Loving every breath of you

:heartpump: :bunnyhug: :yinyang:

:yinyang: :levitate: :earth: :levitate: :yinyang:


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