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OfflineMycomyth
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This seems a little slow...(pics)
    #5443689 - 03/26/06 10:23 AM (17 years, 10 months ago)

First, please excuse the blue and fuzzy pics, no one was in a hurry and the white balance was set wrong.

No one started 6 jars on 3/4/06 with syringes from a sponsor (Puerto Rican and Pink Buff, 3 jars each). No one was a bit (well actually, very) disappointed in these particular syringes. So far, out of 16 cc, only two starts. And those are only PR, no PB has started. They also seemed to take forever to start (~9-10 days). The dark line on the outside of the jar was made on 3/22, photo taken 3/25. These are your standard brf cakes in 1/2 pints. Does this seem slow, or is no one just being impatient?






The rest hadn't started by 3/15, so no one hit them again, and made 3 more each (they were still clean, no contams in any jars...not bad for first try  :shocked:)
By 3/22, no one had another pregnant PR jar with a spot the size of the drawn circle there (still no PB's. No one has injected the last 4 cc of PB into a Karo jar in hopes of salvaging at least something from it)




When these two cakes are done, would it be worthwhile to capture the bits of myc that cover the sides of the jar into a liquid culture? I've been reading up on it, and it seems to me that it, indeed, would be.




Well, thanks in advance for any opinions.  :heart:

Myc


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InvisibleRickster
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Re: This seems a little slow...(pics) [Re: Mycomyth]
    #5443709 - 03/26/06 10:30 AM (17 years, 10 months ago)

Looks alright to me, I don't think it is too slow, if it is slow it could be your temp, cool temp = slower colonization time


Edited by Rickster (03/26/06 10:32 AM)


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OfflineMycomyth
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Re: This seems a little slow...(pics) [Re: Rickster]
    #5443724 - 03/26/06 10:36 AM (17 years, 10 months ago)

Funny you should mention that. No one was reading some posts last night, and realized temp may have been a bit high. No one was keeping the incubator floor temp around 84?F with a TiT. Temp has now been set back, and no one will keep it at around 75-78?F floor temp.

Anyone else?

Myc


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Wave upon wave of demented avengers marched cheerfully out of obscurity into the dream.


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OfflineNunbuh_Chrubble
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Re: This seems a little slow...(pics) [Re: Rickster]
    #5443726 - 03/26/06 10:38 AM (17 years, 10 months ago)

yeah, could be temps, but it could also be CO2. Have you tried loosening the caps/taking the tape off the holes and turning them upside down?

It does seem a little slow. All my jars would at least be half way done in 20 days.

You'll always have slow germination with multi-spore innoc, so your 9~10 days seems normal... but that is some slow colonization.

What you should've done first is make LC's of both syringes before you went all willy nilly throwing the shit away with multi-spore innocs.

ATTENTION ALL NEWBS! SPORES ARE FOR MAKING LC'S, NOT inoculation!
LC > MULTI-SPORE!



--------------------


"This day is a lover..."

~Rumi


Edited by Nunbuh_Chrubble (03/26/06 10:39 AM)


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OfflineMycomyth
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Re: This seems a little slow...(pics) [Re: Nunbuh_Chrubble]
    #5443752 - 03/26/06 10:53 AM (17 years, 10 months ago)

Well, there is no tape on the holes, just the foil that they were sterilized with. I'll leave 'em upside down for awhile, but I don't know if that's the problem or not. The bottom is colonizing nearly as fast as the side.

And...EASE UP! I bet you didn't know everything on your first try either. Thanks for the help anyway, tho.

M


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Offlinefireworks_godS
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Re: This seems a little slow...(pics) [Re: Mycomyth]
    #5443782 - 03/26/06 11:04 AM (17 years, 10 months ago)

Quote:

Mycomyth said:
Temp has now been set back, and no one will keep it at around 75-78?F floor temp.




Incubation should actually be in the eighties, 84 degrees should have been pretty good, from what I have been reading. :wink:

If your colonization time is slower than normal, it might be that the jars don't have enough moisture? Not sure exactly how no one would determine this, but I'd say that if the colonization slows down even more, it probably wouldn't hurt to take some sterilized water in a syringe and inject some water in.

Nevermind that guy, either, my friend has just started growing in the first time in years, and is starting a few lcs and also some classic pf style jars that he'll inoculate with spores. Try everything and experiment, find out what works for you ('course, lc's should definitely be implemented :grin:).

Good luck! :thumbup:

:headbang: :headbang: :headbang: :satansmoking:
Peace. :mushroom2:


--------------------
:redpanda:
If I should die this very moment
I wouldn't fear
For I've never known completeness
Like being here
Wrapped in the warmth of you
Loving every breath of you

:heartpump: :bunnyhug: :yinyang:

:yinyang: :levitate: :earth: :levitate: :yinyang:


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Offlinefireworks_godS
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Re: This seems a little slow...(pics) [Re: Mycomyth]
    #5443787 - 03/26/06 11:07 AM (17 years, 10 months ago)

Quote:

Mycomyth said:
Well, there is no tape on the holes, just the foil that they were sterilized with.




You included a dry verm layer, though, right? I'd take off the tinfoil, and tape up the holes with micropore tape. That should allow some gas exchange. No need to keep the tinfoil on after you sterilized or pc'ed them, they are just on there then to prevent water from entering the jars. :wink:

:headbang: :headbang: :headbang: :satansmoking:
Peace. :mushroom2:


--------------------
:redpanda:
If I should die this very moment
I wouldn't fear
For I've never known completeness
Like being here
Wrapped in the warmth of you
Loving every breath of you

:heartpump: :bunnyhug: :yinyang:

:yinyang: :levitate: :earth: :levitate: :yinyang:


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OfflineMycomyth
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Re: This seems a little slow...(pics) [Re: fireworks_god]
    #5443803 - 03/26/06 11:18 AM (17 years, 10 months ago)

No ones thinking on the temp is that, no one had read that the myc make a bit of its own heat, so it may have actually been too warm inside the jars, where no one cannot measure the temp.

As for the moisture content, look at that last pic, the bits of myc seem to be imbedded in small amounts of moisture, no one really doesn't think it's too dry, but what does no one know? The RH in the incu is kept well above ambient room RH, so evap shouldn't be a problem.

You say LCs are good...any teks on recovering the strands left behind from a cake birth?

Thanks,
M


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Wave upon wave of demented avengers marched cheerfully out of obscurity into the dream.


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Offlinekungpow
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Re: This seems a little slow...(pics) [Re: fireworks_god]
    #5443810 - 03/26/06 11:21 AM (17 years, 10 months ago)

I would just take off the foil. You could tape them with micropore tape but it isn't nessecary. As long as you have the verm barrier there no contams should be able to get in. I've done over 100 pf jars now and not a single one has had a contamination.

I would also boost the temps up a little bit too. The perfect temp is 86 degrees but since your jars are going to create a little a bit of heat set your TiT to 82-84. You should be fine after that. My jars are usually done in 2 weeks. I did use a weak syringe once and it took almost 3. But you should be good.

~Peace Out


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Monotub #3 - check out my best grow to date!!!!



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Offlinenetherstrain
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Re: This seems a little slow...(pics) [Re: Mycomyth]
    #5443816 - 03/26/06 11:24 AM (17 years, 10 months ago)

looks good, but i agree with the other guys when it could be your incubator temp, or Co2 build up. I cant tell what grade of vermiculite you are using, but there has been alot of talk about fine grade vermiculite not being arable enough resulting in slow colonization time.


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