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RateLimitEnzyme
Stranger


Registered: 03/25/06
Posts: 48
Last seen: 16 years, 11 months
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Genetic Modification
#5443391 - 03/26/06 07:07 AM (17 years, 10 months ago) |
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Anyone have any knowledge on GM of entheogens? Worked with a sequencer before?
I have some questions related to genetic overexpression of the rate limiting enzyme without molesting the original enzyme-substrate relationship. In short, potentiation.
I'm really looking for someone who has tried this before whether or not it was a success or failure.
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PsychoChipmunk
Small, Furry, Disturbed

Registered: 01/08/05
Posts: 389
Loc: A hole in your back yard
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I can't imagine there is much out there in the way of research grants for GM or genome mapping on ethnogens. There's probably a wealth of information out there on corn and soybeans though.
Are you looking for information on a specific metabolic pathway?
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Mitchnast
Toadmonger


Registered: 10/27/99
Posts: 8,656
Loc: Okanagan
Last seen: 16 days, 8 hours
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creating a homunculus?
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Banez
Stranger


Registered: 09/23/05
Posts: 15,181
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Re: Genetic Modification [Re: Mitchnast]
#5444299 - 03/26/06 03:14 PM (17 years, 10 months ago) |
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what exactly are you trying to do? i dont know much about enthogens in particular.. however i am a bit of a biology wizard.. specially in genetics.
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RateLimitEnzyme
Stranger


Registered: 03/25/06
Posts: 48
Last seen: 16 years, 11 months
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Re: Genetic Modification [Re: Banez]
#5445148 - 03/26/06 08:17 PM (17 years, 10 months ago) |
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I'm really just looking for anyone else's experiences as there isn't a wealth of information availiable on the subject.
when I have a some spare time, I'm working on overexpressing the rate-limiting enzyme in a few different biosynthetic passageways. Potentiation.
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thedudenj
Man of the Woods

Registered: 08/18/04
Posts: 14,684
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yeah we need to GM entheogens but would that take the soul out of them or would having a DMT,Mescaline,Muscurine,harmaline cactus or leaf have more spirit lol
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  "You all are just puppets... You have no heart...and cannot feel any pain..."" you may think thats pain you feel but you must have a heart to feel true pain and that pain wont be yours
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PsychoChipmunk
Small, Furry, Disturbed

Registered: 01/08/05
Posts: 389
Loc: A hole in your back yard
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What entheogens are you wanting to focus on?
I do agree with thedude on this one. Nature made these plants/fungi in such a fashion that they have something to offer. Trying to play around with them is like asking you buddy to loaned you a fiver to give you a c-note instead. My two cents 
That said, good luck with your project!
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RateLimitEnzyme
Stranger


Registered: 03/25/06
Posts: 48
Last seen: 16 years, 11 months
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Is the corn and soy that you consume missing a soul? Those have been selectively bred for some time. I'm just rushing the selection process. I'm not trying to build a "super-plant" either, as I stated before I'm not interested in molesting the original enzyme-substrate relationship, as this would take years of work.
Think about more potent HBWR, MG (lsa), San Pedro (mesc), or my personal favorite, cannabis (thc).
Edited by RateLimitEnzyme (03/27/06 08:27 AM)
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PsychoChipmunk
Small, Furry, Disturbed

Registered: 01/08/05
Posts: 389
Loc: A hole in your back yard
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There is a fair bit of difference between selective breeding and GM. Why do you think there are so many out there (myself included) that go to great lengths to avoid consuming true GM products? While it can be argued that selective breeding is a form of genetic modification, GM is more commonly understood to involve work involving tinkering with base pair sequences (which can have unforeseen consequences.)
Cannabis HAS been bred for potency over the years. Cacti can be environmentally stressed during growth to produce higher amounts of mescaline. If potency is such an issue, why not just smoke/vaporize another bowl or extract more cacti or seeds?
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RateLimitEnzyme
Stranger


Registered: 03/25/06
Posts: 48
Last seen: 16 years, 11 months
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Extracting LSD-25 from MG would be quite possible.
To trip on LSD, you are ingesting 100-200 micrograms that is .0001 to .0002 grams. With MG, you are ingesting 20 to 40 grams just to get assumably the same amount of LSA. Therefore, you are ingesting 400,000 times more matter than you need to. Once you taste MG, you will become instantly convinced that the less you have to force down the better.
Potentiation would yield an actual extraction method, or the abilty to consume less while receiving more of the desired effect.
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PsychoChipmunk
Small, Furry, Disturbed

Registered: 01/08/05
Posts: 389
Loc: A hole in your back yard
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For the record, you cannot extract LSD-25 from anything: it is a man made synthetic.
Potentiation would not yield an extraction method. Potentiation as you seem to be referring to it aims to increase the concentration of the active components in an entheogen. While this means you would have to consume less raw material, the whole purpose of extraction is to avoid choking down seeds or cactus flesh. You extract the actives and discard as much of the inactive material as possible.
I still haven't figured out if you're really talking about GM or just selective breeding. In any event, good luck with either/or.
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RateLimitEnzyme
Stranger


Registered: 03/25/06
Posts: 48
Last seen: 16 years, 11 months
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Apologies, I meant, ergine/d-lysergic acid amide; which once extracted is easily synthesized into d-lysergic acid diethylamide.
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